دورية أكاديمية
أعرض في EDS

Evaluation of a sequential enzyme immunoassay testing algorithm for Lyme disease demonstrates lack of test independence but high diagnostic specificity.

التفاصيل البيبلوغرافية
العنوان: Evaluation of a sequential enzyme immunoassay testing algorithm for Lyme disease demonstrates lack of test independence but high diagnostic specificity.
المؤلفون: Wormser GP; Division of Infectious Diseases, New York Medical College, Valhalla, NY 10595. Electronic address: gwormser@nymc.edu., Molins CR; Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado., Levin A; Immunetics, Inc., Boston, MA 02210., Lipsett SC; Division of Emergency Medicine, Boston Children's Hospital, 300 Longwood Avenue, Boston, MA 02115., Nigrovic LE; Division of Emergency Medicine, Boston Children's Hospital, 300 Longwood Avenue, Boston, MA 02115., Schriefer ME; Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado., Branda JA; Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA.
المصدر: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2018 Jul; Vol. 91 (3), pp. 217-219. Date of Electronic Publication: 2018 Feb 16.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Biomedical Country of Publication: United States NLM ID: 8305899 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0070 (Electronic) Linking ISSN: 07328893 NLM ISO Abbreviation: Diagn Microbiol Infect Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [New York, NY] : Elsevier Biomedical, [c1983-
مواضيع طبية MeSH: Algorithms*, Antibodies, Bacterial/*blood , Immunoenzyme Techniques/*methods , Lyme Disease/*diagnosis, Borrelia burgdorferi ; Humans ; Immunoglobulin G/blood ; Immunoglobulin M/blood ; Sensitivity and Specificity
مستخلص: To diagnose Lyme disease, a two-tier testing algorithm is used in which supplemental IgM and IgG immunoblots to detect antibody to Borrelia burgdorferi are reflexively performed if a first-tier assay, such as a whole-cell sonicate-based enzyme immunoassay (WCS EIA), is reactive. Recent data suggest that equal specificity is found by substituting the C6 peptide EIA for immunoblots. In this study using 3956 control sera, we demonstrated that although this two-tier testing algorithm does significantly improve diagnostic specificity compared with each of the EIAs individually, the WCS EIA and the C6 peptide EIA are not independent tests. Therefore, when the C6 peptide EIA is used as the second-tier test, it should be regarded as a supplemental rather than a confirmatory test.
(Copyright © 2018. Published by Elsevier Inc.)
References: J Clin Microbiol. 1999 Dec;37(12):3997-4004. (PMID: 10565921)
Clin Infect Dis. 2000 Mar;30(3):545-8. (PMID: 10722442)
Biometrics. 2001 Dec;57(4):1009-17. (PMID: 11764239)
Infect Immun. 2003 Jun;71(6):3419-28. (PMID: 12761126)
Clin Vaccine Immunol. 2007 Aug;14(8):931-6. (PMID: 17567769)
Clin Infect Dis. 2011 Sep;53(6):541-7. (PMID: 21865190)
Clin Microbiol Infect. 2012 Dec;18(12):1236-40. (PMID: 22369185)
Diagn Microbiol Infect Dis. 2013 Jan;75(1):9-15. (PMID: 23062467)
J Clin Microbiol. 2013 Dec;51(12):4045-9. (PMID: 24068010)
J Immunol Methods. 2014 Jan 31;403(1-2):26-36. (PMID: 24287423)
Clin Infect Dis. 2016 Oct 1;63(7):922-8. (PMID: 27358358)
J Clin Microbiol. 2016 Nov;54(11):2726-2734. (PMID: 27558183)
MMWR Morb Mortal Wkly Rep. 1995 Aug 11;44(31):590-1. (PMID: 7623762)
Stat Med. 1998 Apr 30;17(8):873-90. (PMID: 9595617)
معلومات مُعتمدة: CC999999 United States Intramural CDC HHS
فهرسة مساهمة: Keywords: Borrelia burgdorferi; C6 Lyme test; Lyme disease; Test independence; Two-tier serology
المشرفين على المادة: 0 (Antibodies, Bacterial)
0 (Immunoglobulin G)
0 (Immunoglobulin M)
تواريخ الأحداث: Date Created: 20180319 Date Completed: 20180928 Latest Revision: 20200225
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC6519922
DOI: 10.1016/j.diagmicrobio.2018.02.006
PMID: 29550060
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0070
DOI:10.1016/j.diagmicrobio.2018.02.006