دورية أكاديمية
أعرض في EDS

Detection and proteomic characterization of extracellular vesicles in human pancreatic juice.

التفاصيل البيبلوغرافية
العنوان: Detection and proteomic characterization of extracellular vesicles in human pancreatic juice.
المؤلفون: Osteikoetxea X; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary. Electronic address: xabier@med.semmelweis-univ.hu., Benke M; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary; 1st Department of Surgery, Semmelweis University, Budapest, Hungary., Rodriguez M; Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital-The Norwegian Radium Hospital, Oslo, Norway., Pálóczi K; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary., Sódar BW; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary., Szvicsek Z; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary., Szabó-Taylor K; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary., Vukman KV; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary., Kittel Á; Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary., Wiener Z; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary., Vékey K; Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest Hungary., Harsányi L; 1st Department of Surgery, Semmelweis University, Budapest, Hungary., Szűcs Á; 1st Department of Surgery, Semmelweis University, Budapest, Hungary., Turiák L; Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest Hungary., Buzás EI; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary; MTA-SE Immune-Proteogenomics Extracellular Vesicles Research Group, Budapest, Hungary. Electronic address: buzas.edit@med.semmelweis-univ.hu.
المصدر: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2018 Apr 30; Vol. 499 (1), pp. 37-43. Date of Electronic Publication: 2018 Mar 21.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE
أسماء مطبوعة: Publication: <2002- >: San Diego, CA : Elsevier
Original Publication: New York, Academic Press.
مواضيع طبية MeSH: Adenocarcinoma/*diagnosis , Biomarkers, Tumor/*genetics , Cystic Fibrosis Transmembrane Conductance Regulator/*genetics , Extracellular Vesicles/*chemistry , Mucins/*genetics , Pancreatic Neoplasms/*diagnosis, ATP Binding Cassette Transporter, Subfamily B/genetics ; ATP Binding Cassette Transporter, Subfamily B/metabolism ; Adenocarcinoma/genetics ; Adenocarcinoma/metabolism ; Adenocarcinoma/pathology ; Biomarkers, Tumor/metabolism ; Cell Line, Tumor ; Cystic Fibrosis Transmembrane Conductance Regulator/metabolism ; Diagnosis, Differential ; Extracellular Vesicles/metabolism ; Gene Expression ; Humans ; Mucins/metabolism ; Pancreas ; Pancreatic Juice/chemistry ; Pancreatic Juice/metabolism ; Pancreatic Neoplasms/genetics ; Pancreatic Neoplasms/metabolism ; Pancreatic Neoplasms/pathology ; Pancreatitis, Chronic/diagnosis ; Pancreatitis, Chronic/genetics ; Pancreatitis, Chronic/metabolism ; Pancreatitis, Chronic/pathology ; Prognosis ; Proteome/genetics ; Proteome/metabolism ; Proteomics
مستخلص: Aims: The prognosis of patients with pancreatic cancer has remained virtually unchanged with a high mortality rate compared to other types of cancers. An earlier detection would provide a time window of opportunity for treatment and prevention of deaths. In the present study we investigated extracellular vesicle (EV)-associated potential biomarkers for pancreatic cancer by directly assessing EV size-based subpopulations in pancreatic juice samples of patients with chronic pancreatitis or pancreatic cancer. In addition, we also studied blood plasma and pancreatic cancer cell line-derived EVs.
Methods: Comparative proteomic analysis was performed of 102 EV preparations from human pancreatic juices, blood, and pancreatic cancer cell lines Capan-1 and MIA PaCa-2. EV preparations were also characterized by electron microscopy, tunable resistive pulse sensing, and flow cytometry.
Results: Here we describe the presence of EVs in human pancreatic juice samples. Pancreatic juice EV-associated proteins that we identified as possible candidate markers for pancreatic cancer included mucins, such as MUC1, MUC4, MUC5AC, MUC6 and MUC16, CFTR, and MDR1 proteins. These candidate biomarkers could also be detected by flow cytometry in EVs found in pancreatic juice and those secreted by pancreatic cancer cell lines.
Conclusions: Together our data show that detection and characterization of EVs directly in pancreatic juice is feasible and may prove to be a valuable source of potential biomarkers of pancreatic cancer.
(Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: Exosomes; Extracellular vesicles; Pancreas; Pancreatic cancer; Tumor markers
المشرفين على المادة: 0 (ABCB1 protein, human)
0 (ATP Binding Cassette Transporter, Subfamily B)
0 (Biomarkers, Tumor)
0 (CFTR protein, human)
0 (Mucins)
0 (Proteome)
126880-72-6 (Cystic Fibrosis Transmembrane Conductance Regulator)
تواريخ الأحداث: Date Created: 20180319 Date Completed: 20180410 Latest Revision: 20181202
رمز التحديث: 20221213
DOI: 10.1016/j.bbrc.2018.03.107
PMID: 29550476
قاعدة البيانات: MEDLINE
الوصف
تدمد:1090-2104
DOI:10.1016/j.bbrc.2018.03.107