دورية أكاديمية
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Antioxidant and Hemolysis Protective Effects of Polyphenol-Rich Extract from Mulberry Fruits.

التفاصيل البيبلوغرافية
العنوان: Antioxidant and Hemolysis Protective Effects of Polyphenol-Rich Extract from Mulberry Fruits.
المؤلفون: Ajay Krishna PG; Lab of Hi-Tech Processing for Sericultural Resources, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China., Sivakumar TR; Lab of Hi-Tech Processing for Sericultural Resources, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China., Jin C; Lab of Hi-Tech Processing for Sericultural Resources, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China., Li SH; Lab of Hi-Tech Processing for Sericultural Resources, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China., Weng YJ; Lab of Hi-Tech Processing for Sericultural Resources, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China., Yin J; Lab of Hi-Tech Processing for Sericultural Resources, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China., Jia JQ; Lab of Hi-Tech Processing for Sericultural Resources, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China.; Lab of Hi-Tech Processing for Sericultural Resources, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China., Wang CY; Department of Biological and Environmental Engineering, Hefei University, Hefei, Anhui 230601, China., Gui ZZ; Lab of Hi-Tech Processing for Sericultural Resources, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China.; Lab of Hi-Tech Processing for Sericultural Resources, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China.
المصدر: Pharmacognosy magazine [Pharmacogn Mag] 2018 Jan-Mar; Vol. 14 (53), pp. 103-109. Date of Electronic Publication: 2018 Feb 20.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: SAGE Publications Country of Publication: United States NLM ID: 101300403 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0973-1296 (Print) Linking ISSN: 09731296 NLM ISO Abbreviation: Pharmacogn Mag Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: 2023- : [Thousand Oaks] : SAGE Publications
Original Publication: 2005-<2009> : Bangalore : Phcog.Net, Al-Ameen College of Pharmacy
مستخلص: Background: Mulberry fruits are a superior source of polyphenol, especially anthocyanins that contribute potentially to the beneficial effects which include reducing the risk of cardiovascular diseases and cancers with antioxidant, anti-inflammatory, and chemoprotective properties.
Objectives: In this study, purification of the polyphenol-rich extract from mulberry fruit (MPE) was purified and assessed the activities of antioxidant and hemolysis protective in vivo and in vitro .
Materials and Methods: Antioxidant activities in vitro was measured by quantifying its 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, reducing power and Fe 2+ -chelating ability. MPE was purified by high-pressure liquid chromatography (HPLC) and analyzed individual polyphenols using liquid chromatography-mass spectrometry (LC-MS)/MS.
Results: The total polyphenol content was 147.69 ± 0.02 mg gallic acid equivalents (GAE)/g dried weight (DW) in the extract and 403.55 ± 0.02 mg GAE/g DW in the purified extract. Further identification by HPLC-ultraviolet-visible and LC-MS/MS analysis indicated in MPE, an anthocyanin compound, cyanidin-3- O -glucoside. With regard to in vitro assays, MPE possessed antioxidant effect, especially in Fe 2+ chelating ability with an IC 50 value of 1.016 mg/mL. The protective effects on mouse red blood cell hemolysis and lipid peroxidation ex vivo were dose and time dependent.
Conclusion: It indicates that MPE could be a good candidate for future biomedical applications to promote human health with limited side effects.
Summary: Mulberry fruit is an excellent source of polyphenols, in particular, anthocyanins, which has infinite health benefits. This study determined the predominant anthocyanin, cyanidin-3-glucoside, could possibly be the rationale behind the antioxidant and antihemolytic effect of MPE. Results indicate that MPE could be a good candidate for future biomedical applications to promote human health with limited side effects. Abbreviations used: MPE: Purification of the polyphenol-rich extract from mulberry fruit, LC-MS: Liquid chromatography-mass spectrometry, HPLC: High-pressure liquid chromatography, DPPH: 2,2-diphenyl-1-picrylhydrazyl scavenging activity, RBC: Red blood cell, GAE: Gallic acid equivalent, FeCl 2 : Ferrous chloride, H 2 O 2 : Hydrogen peroxide, EDTA-2Na: Ethylenediaminetetraacetic acid disodium salt, PBS: Phosphate-buffered saline, TCA: Trichloroacetic acid, TBA: 2-thiobarbituric acid, FeSO 4 : Ferrous sulphate, MDA: Malondialdehyde, V C : Vitamin C, DW: Dried weight.
Competing Interests: There are no conflicts of interest.
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فهرسة مساهمة: Keywords: Antioxidant activity; hemolysis protective; mulberry fruit; polyphenols; purification
تواريخ الأحداث: Date Created: 20180327 Latest Revision: 20220316
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC5858229
DOI: 10.4103/pm.pm_491_16
PMID: 29576709
قاعدة البيانات: MEDLINE
الوصف
تدمد:0973-1296
DOI:10.4103/pm.pm_491_16