دورية أكاديمية
Biochemical bases of growth variation during development: a study of protein turnover in pedigreed families of bivalve larvae ( Crassostrea gigas ).
| العنوان: | Biochemical bases of growth variation during development: a study of protein turnover in pedigreed families of bivalve larvae ( Crassostrea gigas ). |
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| المؤلفون: | Pan TF; Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089-0371, USA., Applebaum SL; Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089-0371, USA., Frieder CA; Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089-0371, USA., Manahan DT; Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089-0371, USA manahan@usc.edu. |
| المصدر: | The Journal of experimental biology [J Exp Biol] 2018 May 20; Vol. 221 (Pt 10). Date of Electronic Publication: 2018 May 20. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Company Of Biologists Limited Country of Publication: England NLM ID: 0243705 Publication Model: Electronic Cited Medium: Internet ISSN: 1477-9145 (Electronic) Linking ISSN: 00220949 NLM ISO Abbreviation: J Exp Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Company Of Biologists Limited Original Publication: London, Cambridge Univ. Press. |
| مواضيع طبية MeSH: | Protein Biosynthesis*, Crassostrea/*growth & development , Crassostrea/*metabolism, Adenosine Triphosphate/metabolism ; Animal Shells/growth & development ; Animals ; Crassostrea/chemistry ; Crassostrea/genetics ; Genotype ; Larva/chemistry ; Larva/genetics ; Larva/growth & development ; Larva/metabolism ; Phenotype |
| مستخلص: | Animal size is a highly variable trait regulated by complex interactions between biological and environmental processes. Despite the importance of understanding the mechanistic bases of growth, predicting size variation in early stages of development remains challenging. Pedigreed lines of the Pacific oyster ( Crassostrea gigas ) were crossed to produce contrasting growth phenotypes to analyze the metabolic bases of growth variation in larval stages. Under controlled environmental conditions, substantial growth variation of up to 430% in shell length occurred among 12 larval families. Protein was the major biochemical constituent in larvae, with an average protein-to-lipid content ratio of 2.8. On average, 86% of protein synthesized was turned over (i.e. only 14% retained as protein accreted), with a regulatory shift in depositional efficiency resulting in increased protein accretion during later larval growth. Variation in protein depositional efficiency among families did not explain the range in larval growth rates. Instead, changes in protein synthesis rates predicted 72% of growth variation. High rates of protein synthesis to support faster growth, in turn, necessitated greater allocation of the total ATP pool to protein synthesis. An ATP allocation model is presented for larvae of C. gigas that includes the major components (82%) of energy demand: protein synthesis (45%), ion pump activity (20%), shell formation (14%) and protein degradation (3%). The metabolic trade-offs between faster growth and the need for higher ATP allocation to protein synthesis could be a major determinant of fitness for larvae of different genotypes responding to the stress of environmental change. Competing Interests: Competing interestsThe authors declare no competing or financial interests. (© 2018. Published by The Company of Biologists Ltd.) |
| فهرسة مساهمة: | Keywords: Development; Larvae; Metabolic trade-offs; Pacific oyster; Pedigreed lines; Protein synthesis |
| المشرفين على المادة: | 8L70Q75FXE (Adenosine Triphosphate) |
| تواريخ الأحداث: | Date Created: 20180405 Date Completed: 20191008 Latest Revision: 20191008 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1242/jeb.171967 |
| PMID: | 29615524 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1477-9145 |
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| DOI: | 10.1242/jeb.171967 |