دورية أكاديمية
أعرض في EDS

Generation and customization of biosynthetic excitable tissues for electrophysiological studies and cell-based therapies.

التفاصيل البيبلوغرافية
العنوان: Generation and customization of biosynthetic excitable tissues for electrophysiological studies and cell-based therapies.
المؤلفون: Nguyen HX; Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA., Kirkton RD; Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA., Bursac N; Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA.
المصدر: Nature protocols [Nat Protoc] 2018 May; Vol. 13 (5), pp. 927-945. Date of Electronic Publication: 2018 Apr 05.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101284307 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1750-2799 (Electronic) Linking ISSN: 17502799 NLM ISO Abbreviation: Nat Protoc Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London, UK : Nature Pub. Group, 2006-
مواضيع طبية MeSH: Action Potentials* , Electric Stimulation*, Cell Engineering/*methods , Ion Channels/*metabolism , Mutant Proteins/*metabolism, Cells, Cultured ; Fibroblasts/physiology ; Humans ; Ion Channels/genetics ; Mutant Proteins/genetics ; Patch-Clamp Techniques
مستخلص: We describe a two-stage protocol to generate electrically excitable and actively conducting cell networks with stable and customizable electrophysiological phenotypes. Using this method, we have engineered monoclonally derived excitable tissues as a robust and reproducible platform to investigate how specific ion channels and mutations affect action potential (AP) shape and conduction. In the first stage of the protocol, we combine computational modeling, site-directed mutagenesis, and electrophysiological techniques to derive optimal sets of mammalian and/or prokaryotic ion channels that produce specific AP shape and conduction characteristics. In the second stage of the protocol, selected ion channels are stably expressed in unexcitable human cells by means of viral or nonviral delivery, followed by flow cytometry or antibiotic selection to purify the desired phenotype. This protocol can be used with traditional heterologous expression systems or primary excitable cells, and application of this method to primary fibroblasts may enable an alternative approach to cardiac cell therapy. Compared with existing methods, this protocol generates a well-defined, relatively homogeneous electrophysiological phenotype of excitable cells that facilitates experimental and computational studies of AP conduction and can decrease arrhythmogenic risk upon cell transplantation. Although basic cell culture and molecular biology techniques are sufficient to generate excitable tissues using the described protocol, experience with patch-clamp techniques is required to characterize and optimize derived cell populations.
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معلومات مُعتمدة: R01 HL132389 United States HL NHLBI NIH HHS; U01 HL134764 United States HL NHLBI NIH HHS; R21 HL095069 United States HL NHLBI NIH HHS; R21 HL126193 United States HL NHLBI NIH HHS; R21 HL106203 United States HL NHLBI NIH HHS; R21 HL083342 United States HL NHLBI NIH HHS; R01 HL126524 United States HL NHLBI NIH HHS; R01 HL104326 United States HL NHLBI NIH HHS
المشرفين على المادة: 0 (Ion Channels)
0 (Mutant Proteins)
تواريخ الأحداث: Date Created: 20180407 Date Completed: 20190415 Latest Revision: 20240610
رمز التحديث: 20240610
مُعرف محوري في PubMed: PMC6050172
DOI: 10.1038/nprot.2018.016
PMID: 29622805
قاعدة البيانات: MEDLINE
الوصف
تدمد:1750-2799
DOI:10.1038/nprot.2018.016