دورية أكاديمية
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Palmitoyl-ceramide accumulation with necrotic cell death in A549 cells, followed by a steep increase in sphinganine content.

التفاصيل البيبلوغرافية
العنوان: Palmitoyl-ceramide accumulation with necrotic cell death in A549 cells, followed by a steep increase in sphinganine content.
المؤلفون: Yamane M; Department of Biochemistry, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-0023, Japan.
المصدر: Biochimie open [Biochim Open] 2015 Jun 21; Vol. 1, pp. 11-27. Date of Electronic Publication: 2015 Jun 21 (Print Publication: 2015).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Published by Elsevier B.V. on behalf of Société Française de Biochimie et Biologie Moléculaire Country of Publication: Netherlands NLM ID: 101706117 Publication Model: eCollection Cited Medium: Internet ISSN: 2214-0085 (Electronic) Linking ISSN: 22140085 NLM ISO Abbreviation: Biochim Open Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [Amsterdam] : Published by Elsevier B.V. on behalf of Société Française de Biochimie et Biologie Moléculaire, [2015]-[2018]
مستخلص: Ceramides (Cers) have recently been identified as key signaling molecules that mediate biological functions such as cell growth, differentiation, senescence, apoptosis, and autophagy. However, the functions of Cer accumulation in necrotic cell death remain unknown. The aim of this study was to clarify the relationship between Cer accumulation with inhibition of the conversion pathway of Cer and concomitant necrotic cell death. In order to minimize the effect of apoptosis against necrotic cell death, A549 cells having the inhibiting effect of caspase 9 by survivin were used in this study. Consequently, Cer accumulation in A549 cells would likely be associated with a pathway other than the mitochondrial caspase-dependent pathway of apoptosis. Here, we showed that the dual addition of a glucosyl-Cer synthase inhibitor and a ceramidase inhibitor to A549 cell culture induced palmitoyl-Cer accumulation with Cer synthase 5 expression and necrotic cell death with lysosomal rupture together with leakage of cathepsin B/alkalization after 2-3 h, although it is unknown in this study whether the necrotic cell death was caused by the lysosomal rupture. This Cer accumulation was followed by a steep increase in sphinganine base levels via the activation of serine palmitoyltransferase activity brought about by the increase in palmitoyl-coenzyme A concentration as a substrate after 5-6 h. The increase in palmitoyl-coenzyme A concentration was achieved by activation of the fatty acid synthetic pathway from acetyl coenzyme A.
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فهرسة مساهمة: Keywords: ([13C16]C16:0-CoA, palmitoyl-13C16 coenzyme A; 4-HPR, N-(4-hydroxyphenyl)retinamide; A549 cells; APCI, atmospheric pressure chemical ionization; BSA, bovine serum albumin; C16:0-Cer, palmitoyl-ceramide; C16:0-CoA, palmitoyl-coenzyme A; C2:0-CoA, acetyl-coenzyme A; CHOP, CAAT/enhancer binding protein homologous protein; CathB, cathepsin B; Cer, ceramide; CerS, ceramide synthase; D-NMAPPD; D-NMAPPD, N-[(1R,2R)-2-hydroxy-1-(hydroxy-methyl)-2-(4-nitrophenyl)ethyl]tetradecanamide; DAPI, 4′,6-diamidino-2-phenylindole; DL-PDMP; DL-PDMP, DL-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol; DMEM, Dulbecco's modified Eagle's medium; DMSO, dimethylsulfoxide; DTT, dithiothreitol; ER, endoplasmic reticulum; ESI, electrospray ionization; FATP1, fatty acid transport protein 1; FBS, fetal bovine serum; GlcCer, glucosylceramide; IS, internal standard; L-[2,3,3-D3]Ser, L-serine-2,3,3-D3; LC3, microtubule-associated protein 1 light chain 3B; LDH, lactate dehydrogenase; LMP, lysosomal membrane permeabilization; Lys, lysosomes; MAM, mitochondria-associated membrane; Myriocin, 2-amino-3,4-dihydroxy-2-(hydroxymethyl)-14-oxo-6-eicosenoic acid; Necrosis; Palmitoyl-ceramide; SDS, sodium dodecyl sulfate; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; SIM, selected-ion monitoring; SM, sphingomyelin; SPT, serine palmitoyltransferase; SPTLC, SPT-long chain base subunit; Ser, Serine; Sphinganine; [1,2,3,4-13C4]C16:0 acid, palmitic acid-1,2,3,4-13C4; [2-13C]C2:0 acid, sodium acetate-2-13C; [D7]d18:0, D-erythro-sphinganine-D7; [D7]d18:1, D-erythro-sphingosine-D7; acridine orange, 3,6-Bis(dimethylamino) acridine hydrochloride; d18:0, sphinganine; d18:1, sphingosine; d18:1-[D31]C16:0-Cer, N-palmitoyl [D31]-D-erythro-sphingosine
تواريخ الأحداث: Date Created: 20180411 Latest Revision: 20200929
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC5889477
DOI: 10.1016/j.biopen.2015.06.001
PMID: 29632826
قاعدة البيانات: MEDLINE
الوصف
تدمد:2214-0085
DOI:10.1016/j.biopen.2015.06.001