دورية أكاديمية
A comprehensive web tool for toehold switch design.
| العنوان: | A comprehensive web tool for toehold switch design. |
|---|---|
| المؤلفون: | To AC; School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong., Chu DH; School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong., Wang AR; School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong., Li FC; Department of Computer Science and Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong., Chiu AW; Department of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong., Gao DY; Department of Computer Science and Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.; Department of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong., Choi CHJ; Department of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong., Kong SK; School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong., Chan TF; School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong., Chan KM; School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong., Yip KY; Department of Computer Science and Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.; Department of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong. |
| المصدر: | Bioinformatics (Oxford, England) [Bioinformatics] 2018 Aug 15; Vol. 34 (16), pp. 2862-2864. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 9808944 Publication Model: Print Cited Medium: Internet ISSN: 1367-4811 (Electronic) Linking ISSN: 13674803 NLM ISO Abbreviation: Bioinformatics Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Oxford : Oxford University Press, c1998- |
| مواضيع طبية MeSH: | Software Design*, Binding Sites ; Nucleic Acid Conformation ; RNA/chemistry ; Ribosomes/metabolism |
| مستخلص: | Motivation: Toehold switches are a class of RNAs with a hairpin loop that can be unfolded upon binding a trigger RNA, thereby exposing a ribosome binding site (RBS) and permitting translation of the reporter protein. They have been shown very useful in detecting a variety of targets including RNAs from Zika and Ebola viruses. The base complementation between the toehold switch and the trigger RNA also makes it sensitive to sequence variations. Design of toehold switches involves a series of considerations related to their sequence properties, structures and specificities. Results: Here we present the first comprehensive web tool for designing toehold switches. We also propose a score for predicting the efficacy of designed toehold switches based on properties learned from ∼180 experimentally tested switches. Availability and Implementation: The toehold switch web tool is available at https://yiplab.cse.cuhk.edu.hk/toehold/. |
| المشرفين على المادة: | 63231-63-0 (RNA) |
| تواريخ الأحداث: | Date Created: 20180413 Date Completed: 20190923 Latest Revision: 20190923 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1093/bioinformatics/bty216 |
| PMID: | 29648573 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1367-4811 |
|---|---|
| DOI: | 10.1093/bioinformatics/bty216 |