دورية أكاديمية
Insulin-induced exocytosis regulates the cell surface level of low-density lipoprotein-related protein-1 in Müller Glial cells.
| العنوان: | Insulin-induced exocytosis regulates the cell surface level of low-density lipoprotein-related protein-1 in Müller Glial cells. |
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| المؤلفون: | Actis Dato V; Universidad Nacional de Córdoba, Facultad de Ciencias Químicas, Departamento de Bioquímica Clínica, Córdoba, Argentina.; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET),Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), Córdoba, Argentina., Grosso RA; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Histología y Embriología (IHEM), Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, Argentina., Sánchez MC; Universidad Nacional de Córdoba, Facultad de Ciencias Químicas, Departamento de Bioquímica Clínica, Córdoba, Argentina.; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET),Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), Córdoba, Argentina., Fader CM; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Histología y Embriología (IHEM), Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, Argentina.; Universidad Nacional de Cuyo, Facultad de Odontología, Mendoza, Argentina., Chiabrando GA; Universidad Nacional de Córdoba, Facultad de Ciencias Químicas, Departamento de Bioquímica Clínica, Córdoba, Argentina gustavo.chiabrando@unc.edu.ar.; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET),Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), Córdoba, Argentina. |
| المصدر: | The Biochemical journal [Biochem J] 2018 May 15; Vol. 475 (9), pp. 1669-1685. Date of Electronic Publication: 2018 May 15. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published by Portland Press on behalf of the Biochemical Society Country of Publication: England NLM ID: 2984726R Publication Model: Electronic Cited Medium: Internet ISSN: 1470-8728 (Electronic) Linking ISSN: 02646021 NLM ISO Abbreviation: Biochem J Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London, UK : Published by Portland Press on behalf of the Biochemical Society |
| مواضيع طبية MeSH: | Gene Expression Regulation*, Cell Membrane/*metabolism , Ependymoglial Cells/*metabolism , Exocytosis/*drug effects , Insulin/*pharmacology , Low Density Lipoprotein Receptor-Related Protein-1/*metabolism, Adaptor Proteins, Vesicular Transport/metabolism ; Cells, Cultured ; Ependymoglial Cells/drug effects ; Glucose Transporter Type 4/metabolism ; Humans ; Hypoglycemic Agents/pharmacology ; Protein Transport ; Signal Transduction ; rab GTP-Binding Proteins/metabolism |
| مستخلص: | Low-density lipoprotein (LDL) receptor-related protein-1 (LRP1) is expressed in retinal Müller glial cells (MGCs) and regulates intracellular translocation to the plasma membrane (PM) of the membrane proteins involved in cellular motility and activity. Different functions of MGCs may be influenced by insulin, including the removal of extracellular glutamate in the retina. In the present work, we investigated whether insulin promotes LRP1 translocation to the PM in the Müller glial-derived cell line MIO-M1 (human retinal Müller glial cell-derived cell line). We demonstrated that LRP1 is stored in small vesicles containing an approximate size of 100 nm (mean diameter range of 100-120 nm), which were positive for sortilin and VAMP2, and also incorporated GLUT4 when it was transiently transfected. Next, we observed that LRP1 translocation to the PM was promoted by insulin-regulated exocytosis through intracellular activation of the IR/PI (© 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.) |
| فهرسة مساهمة: | Keywords: GTPases; endocytosis; intracellular signaling; membranes; trafficking |
| المشرفين على المادة: | 0 (Adaptor Proteins, Vesicular Transport) 0 (Glucose Transporter Type 4) 0 (Hypoglycemic Agents) 0 (Insulin) 0 (LRP1 protein, human) 0 (Low Density Lipoprotein Receptor-Related Protein-1) 0 (SLC2A4 protein, human) EC 3.6.5.2 (rab GTP-Binding Proteins) Z020Y8WIJ4 (sortilin) |
| تواريخ الأحداث: | Date Created: 20180420 Date Completed: 20190212 Latest Revision: 20190215 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1042/BCJ20170891 |
| PMID: | 29669912 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1470-8728 |
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| DOI: | 10.1042/BCJ20170891 |