دورية أكاديمية
Optimisation of quantitative miRNA panels to consolidate the diagnostic surveillance of HBV-related hepatocellular carcinoma.
العنوان: | Optimisation of quantitative miRNA panels to consolidate the diagnostic surveillance of HBV-related hepatocellular carcinoma. |
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المؤلفون: | Tat Trung N; Department of Molecular Biology, 108 Military Central Hospital, Hanoi, Vietnam.; Department of Gastroenterology, 108 Military Central Hospital, Hanoi, Vietnam.; Vietnamese-German Center of Excellence in Medical Research, Hanoi, Vietnam., Duong DC; Department of Molecular Biology, 108 Military Central Hospital, Hanoi, Vietnam.; Department of Pathophysiology, Vietnam Military Medical University, Hanoi, Vietnam.; Viet Tiep Hospital, Le Chan District, Hai Phong, Vietnam., Tong HV; Vietnamese-German Center of Excellence in Medical Research, Hanoi, Vietnam.; Department of Pathophysiology, Vietnam Military Medical University, Hanoi, Vietnam.; Institute of Biomedicine and Pharmacy, Vietnam Military Medical University, Hanoi, Vietnam., Hien TTT; Department of Molecular Biology, 108 Military Central Hospital, Hanoi, Vietnam., Hoan PQ; Department of Molecular Biology, 108 Military Central Hospital, Hanoi, Vietnam.; Vietnamese-German Center of Excellence in Medical Research, Hanoi, Vietnam., Bang MH; Department of Gastroenterology, 108 Military Central Hospital, Hanoi, Vietnam.; Vietnamese-German Center of Excellence in Medical Research, Hanoi, Vietnam., Binh MT; Department of Gastroenterology, 108 Military Central Hospital, Hanoi, Vietnam.; Vietnamese-German Center of Excellence in Medical Research, Hanoi, Vietnam.; Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany., Ky TD; Department of Gastroenterology, 108 Military Central Hospital, Hanoi, Vietnam., Tung NL; Department of Gastroenterology, 108 Military Central Hospital, Hanoi, Vietnam., Thinh NT; Department of Gastroenterology, 108 Military Central Hospital, Hanoi, Vietnam., Sang VV; Vietnamese-German Center of Excellence in Medical Research, Hanoi, Vietnam.; Institute of Clinical Infectious Diseases, 108 Military Central Hospital, Hanoi, Vietnam., Thao LTP; Department of Pharmacy, 108 Military Central Hospital, Hanoi, Vietnam., Bock CT; Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany.; Department of Infectious Diseases, Robert Koch Institute, Berlin, Germany., Velavan TP; Vietnamese-German Center of Excellence in Medical Research, Hanoi, Vietnam.; Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany.; Duy Tan University, Dan Nang, Vietnam., Meyer CG; Vietnamese-German Center of Excellence in Medical Research, Hanoi, Vietnam.; Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany.; Duy Tan University, Dan Nang, Vietnam., Song LH; Department of Molecular Biology, 108 Military Central Hospital, Hanoi, Vietnam.; Vietnamese-German Center of Excellence in Medical Research, Hanoi, Vietnam.; Institute of Clinical Infectious Diseases, 108 Military Central Hospital, Hanoi, Vietnam., Toan NL; Vietnamese-German Center of Excellence in Medical Research, Hanoi, Vietnam.; Department of Pathophysiology, Vietnam Military Medical University, Hanoi, Vietnam. |
المصدر: | PloS one [PLoS One] 2018 Apr 19; Vol. 13 (4), pp. e0196081. Date of Electronic Publication: 2018 Apr 19 (Print Publication: 2018). |
نوع المنشور: | Journal Article; Meta-Analysis; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: San Francisco, CA : Public Library of Science |
مواضيع طبية MeSH: | Carcinoma, Hepatocellular/*diagnosis , Carcinoma, Hepatocellular/*etiology , Hepatitis B, Chronic/*complications , Liver Neoplasms/*diagnosis , Liver Neoplasms/*etiology , MicroRNAs/*genetics, Adult ; Aged ; Aged, 80 and over ; Biomarkers, Tumor ; Case-Control Studies ; Disease Susceptibility ; Early Detection of Cancer/methods ; Female ; Gene Expression Profiling/methods ; Genetic Testing ; Hepatitis B, Chronic/diagnosis ; Hepatitis B, Chronic/virology ; Humans ; Male ; Middle Aged ; Prognosis ; ROC Curve ; Serologic Tests ; Young Adult |
مستخلص: | Background: Circulating microRNAs (miRNA) are biomarkers for several neoplastic diseases, including hepatocellular carcinoma (HCC). We performed a literature search, followed by experimental screening and validation in order to establish a miRNA panel in combination with the assessment of alpha-fetoprotein (AFP) levels and to evaluate its performance in HCC diagnostics. Methods: Expression of miRNAs was quantified by quantitative PCR (qPCR) in 406 serum samples from 118 Vietnamese patients with hepatitis B (HBV)-related HCC, 69 patients with HBV-related liver cirrhosis (LC), 100 chronic hepatitis B (CHB) patients and 119 healthy controls (HC). Results: Three miRNAs (mir-21, mir-122, mir-192) were expressed differentially among the studied subgroups and positively correlated with AFP levels. The individual miRNAs mir-21, mir-122, mir192 or the triplex miRNA panel showed high diagnostic accuracy for HCC (HCC vs. CHB, AUC = 0.906; HCC vs. CHB+LC, AUC = 0.81; HCC vs. CHB+LC+HC, AUC = 0.854). When AFP levels were ≤20ng/ml, the triplex miRNA panel still was accurate in distinguishing HCC from the other conditions (CHB, AUC = 0.922; CHB+LC, AUC = 0.836; CHB+LC+HC, AUC = 0.862). When AFP levels were used in combination with the triplex miRNA panel, the diagnostic performance was significantly improved in discriminating HCC from the other groups (LC, AUC = 0.887; CHB, AUC = 0.948; CHB+LC, AUC = 0.887). Conclusions: The three miRNAs mir-21, mir-122, mir-192, together with AFP, are biomarkers that may be applied to improve diagnostics of HCC in HBV patients, especially in HBV-related LC patients with normal AFP levels or HCC patients with small tumor sizes. |
References: | Liver Int. 2014 Feb;34(2):281-95. (PMID: 23834235) RNA. 2008 May;14(5):844-52. (PMID: 18375788) Nucleic Acids Res. 2005 Nov 27;33(20):e179. (PMID: 16314309) Gastroenterology. 2004 Nov;127(5 Suppl 1):S113-9. (PMID: 15508074) Lancet. 2012 Mar 31;379(9822):1245-55. (PMID: 22353262) Methods. 2001 Dec;25(4):402-8. (PMID: 11846609) Eur J Cancer. 2012 Mar;48(5):599-641. (PMID: 22424278) J Gastroenterol Hepatol. 1998 Jul;13(7):685-90. (PMID: 9715418) Am J Gastroenterol. 2011 Sep;106(9):1663-9. (PMID: 21606975) Nat Rev Cancer. 2015 Jun;15(6):321-33. (PMID: 25998712) Clin Chem. 2010 Dec;56(12 ):1830-8. (PMID: 20930130) Nat Rev Cancer. 2015 Nov;15(11):653-67. (PMID: 26493646) J Clin Oncol. 2011 Dec 20;29(36):4781-8. (PMID: 22105822) PLoS One. 2011;6(12):e28486. (PMID: 22174818) Aliment Pharmacol Ther. 2009 Jul;30(1):37-47. (PMID: 19392863) Hepatology. 2011 Mar;53(3):1060-1; author reply 1061-2. (PMID: 21374678) Oncology. 2007;72 Suppl 1:117-23. (PMID: 18087192) Clin Transl Oncol. 2015 Sep;17(9):684-93. (PMID: 25956842) Nat Rev Clin Oncol. 2014 Sep;11(9):525-35. (PMID: 25091611) J Cell Physiol. 2016 Feb;231(2):328-35. (PMID: 26291451) Lancet Oncol. 2012 Aug;13(8):750-1. (PMID: 22738800) Oncotarget. 2016 May 31;7(22):32607-16. (PMID: 27119355) J Cell Biochem. 2014 Sep;115(9):1582-93. (PMID: 24733606) Lancet Oncol. 2015 Jul;16(7):804-15. (PMID: 26088272) J Hepatol. 2012 Jan;56(1):167-75. (PMID: 21749846) Curr Gene Ther. 2015;15(2):142-50. (PMID: 25537773) Arch Intern Med. 2008 Mar 24;168(6):663-6. (PMID: 18362260) Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4402-7. (PMID: 19246379) J Clin Virol. 2003 Sep;28(1):93-103. (PMID: 12927756) J Hepatol. 2014 Dec;61(6):1434-7. (PMID: 25306489) Liver Cancer. 2015 Mar;4(2):85-95. (PMID: 26020031) HPB (Oxford). 2005;7(1):35-41. (PMID: 18333159) N Engl J Med. 2011 Sep 22;365(12):1118-27. (PMID: 21992124) Proc Natl Acad Sci U S A. 2008 Jul 29;105(30):10513-8. (PMID: 18663219) |
المشرفين على المادة: | 0 (Biomarkers, Tumor) 0 (MicroRNAs) |
تواريخ الأحداث: | Date Created: 20180420 Date Completed: 20180723 Latest Revision: 20181114 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC5908085 |
DOI: | 10.1371/journal.pone.0196081 |
PMID: | 29672637 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1932-6203 |
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DOI: | 10.1371/journal.pone.0196081 |