دورية أكاديمية
أعرض في EDS

Optimisation of quantitative miRNA panels to consolidate the diagnostic surveillance of HBV-related hepatocellular carcinoma.

التفاصيل البيبلوغرافية
العنوان: Optimisation of quantitative miRNA panels to consolidate the diagnostic surveillance of HBV-related hepatocellular carcinoma.
المؤلفون: Tat Trung N; Department of Molecular Biology, 108 Military Central Hospital, Hanoi, Vietnam.; Department of Gastroenterology, 108 Military Central Hospital, Hanoi, Vietnam.; Vietnamese-German Center of Excellence in Medical Research, Hanoi, Vietnam., Duong DC; Department of Molecular Biology, 108 Military Central Hospital, Hanoi, Vietnam.; Department of Pathophysiology, Vietnam Military Medical University, Hanoi, Vietnam.; Viet Tiep Hospital, Le Chan District, Hai Phong, Vietnam., Tong HV; Vietnamese-German Center of Excellence in Medical Research, Hanoi, Vietnam.; Department of Pathophysiology, Vietnam Military Medical University, Hanoi, Vietnam.; Institute of Biomedicine and Pharmacy, Vietnam Military Medical University, Hanoi, Vietnam., Hien TTT; Department of Molecular Biology, 108 Military Central Hospital, Hanoi, Vietnam., Hoan PQ; Department of Molecular Biology, 108 Military Central Hospital, Hanoi, Vietnam.; Vietnamese-German Center of Excellence in Medical Research, Hanoi, Vietnam., Bang MH; Department of Gastroenterology, 108 Military Central Hospital, Hanoi, Vietnam.; Vietnamese-German Center of Excellence in Medical Research, Hanoi, Vietnam., Binh MT; Department of Gastroenterology, 108 Military Central Hospital, Hanoi, Vietnam.; Vietnamese-German Center of Excellence in Medical Research, Hanoi, Vietnam.; Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany., Ky TD; Department of Gastroenterology, 108 Military Central Hospital, Hanoi, Vietnam., Tung NL; Department of Gastroenterology, 108 Military Central Hospital, Hanoi, Vietnam., Thinh NT; Department of Gastroenterology, 108 Military Central Hospital, Hanoi, Vietnam., Sang VV; Vietnamese-German Center of Excellence in Medical Research, Hanoi, Vietnam.; Institute of Clinical Infectious Diseases, 108 Military Central Hospital, Hanoi, Vietnam., Thao LTP; Department of Pharmacy, 108 Military Central Hospital, Hanoi, Vietnam., Bock CT; Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany.; Department of Infectious Diseases, Robert Koch Institute, Berlin, Germany., Velavan TP; Vietnamese-German Center of Excellence in Medical Research, Hanoi, Vietnam.; Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany.; Duy Tan University, Dan Nang, Vietnam., Meyer CG; Vietnamese-German Center of Excellence in Medical Research, Hanoi, Vietnam.; Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany.; Duy Tan University, Dan Nang, Vietnam., Song LH; Department of Molecular Biology, 108 Military Central Hospital, Hanoi, Vietnam.; Vietnamese-German Center of Excellence in Medical Research, Hanoi, Vietnam.; Institute of Clinical Infectious Diseases, 108 Military Central Hospital, Hanoi, Vietnam., Toan NL; Vietnamese-German Center of Excellence in Medical Research, Hanoi, Vietnam.; Department of Pathophysiology, Vietnam Military Medical University, Hanoi, Vietnam.
المصدر: PloS one [PLoS One] 2018 Apr 19; Vol. 13 (4), pp. e0196081. Date of Electronic Publication: 2018 Apr 19 (Print Publication: 2018).
نوع المنشور: Journal Article; Meta-Analysis; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Carcinoma, Hepatocellular/*diagnosis , Carcinoma, Hepatocellular/*etiology , Hepatitis B, Chronic/*complications , Liver Neoplasms/*diagnosis , Liver Neoplasms/*etiology , MicroRNAs/*genetics, Adult ; Aged ; Aged, 80 and over ; Biomarkers, Tumor ; Case-Control Studies ; Disease Susceptibility ; Early Detection of Cancer/methods ; Female ; Gene Expression Profiling/methods ; Genetic Testing ; Hepatitis B, Chronic/diagnosis ; Hepatitis B, Chronic/virology ; Humans ; Male ; Middle Aged ; Prognosis ; ROC Curve ; Serologic Tests ; Young Adult
مستخلص: Background: Circulating microRNAs (miRNA) are biomarkers for several neoplastic diseases, including hepatocellular carcinoma (HCC). We performed a literature search, followed by experimental screening and validation in order to establish a miRNA panel in combination with the assessment of alpha-fetoprotein (AFP) levels and to evaluate its performance in HCC diagnostics.
Methods: Expression of miRNAs was quantified by quantitative PCR (qPCR) in 406 serum samples from 118 Vietnamese patients with hepatitis B (HBV)-related HCC, 69 patients with HBV-related liver cirrhosis (LC), 100 chronic hepatitis B (CHB) patients and 119 healthy controls (HC).
Results: Three miRNAs (mir-21, mir-122, mir-192) were expressed differentially among the studied subgroups and positively correlated with AFP levels. The individual miRNAs mir-21, mir-122, mir192 or the triplex miRNA panel showed high diagnostic accuracy for HCC (HCC vs. CHB, AUC = 0.906; HCC vs. CHB+LC, AUC = 0.81; HCC vs. CHB+LC+HC, AUC = 0.854). When AFP levels were ≤20ng/ml, the triplex miRNA panel still was accurate in distinguishing HCC from the other conditions (CHB, AUC = 0.922; CHB+LC, AUC = 0.836; CHB+LC+HC, AUC = 0.862). When AFP levels were used in combination with the triplex miRNA panel, the diagnostic performance was significantly improved in discriminating HCC from the other groups (LC, AUC = 0.887; CHB, AUC = 0.948; CHB+LC, AUC = 0.887).
Conclusions: The three miRNAs mir-21, mir-122, mir-192, together with AFP, are biomarkers that may be applied to improve diagnostics of HCC in HBV patients, especially in HBV-related LC patients with normal AFP levels or HCC patients with small tumor sizes.
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المشرفين على المادة: 0 (Biomarkers, Tumor)
0 (MicroRNAs)
تواريخ الأحداث: Date Created: 20180420 Date Completed: 20180723 Latest Revision: 20181114
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC5908085
DOI: 10.1371/journal.pone.0196081
PMID: 29672637
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0196081