دورية أكاديمية
[Identification of Fusion Transcripts in Leukеmic Cells by Whole-Transcriptome Sequencing].
| العنوان: | [Identification of Fusion Transcripts in Leukеmic Cells by Whole-Transcriptome Sequencing]. |
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| المؤلفون: | Ikonnikova AY; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia., Ammour YI; Federal Research and Clinical Center of Pediatric Hematology, Oncology, and Immunology, Moscow, 119117 Russia., Snezhkina AV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia., Krasnov GS; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia., Kudryavtseva AV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia., Nasedkina TV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.; Federal Research and Clinical Center of Pediatric Hematology, Oncology, and Immunology, Moscow, 119117 Russia.; nased@eimb.ru. |
| المصدر: | Molekuliarnaia biologiia [Mol Biol (Mosk)] 2018 Mar-Apr; Vol. 52 (2), pp. 231-237. |
| نوع المنشور: | Journal Article |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Izdatelstvo Nauka Country of Publication: Russia (Federation) NLM ID: 0105454 Publication Model: Print Cited Medium: Print ISSN: 0026-8984 (Print) Linking ISSN: 00268984 NLM ISO Abbreviation: Mol Biol (Mosk) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Moskva : Izdatelstvo Nauka |
| مواضيع طبية MeSH: | High-Throughput Nucleotide Sequencing* , Leukemia*/genetics , Leukemia*/metabolism , Oncogene Proteins, Fusion*/biosynthesis , Oncogene Proteins, Fusion*/genetics , RNA, Messenger*/biosynthesis , RNA, Messenger*/genetics , RNA, Neoplasm*/biosynthesis , RNA, Neoplasm*/genetics , Transcriptome*, Adolescent ; Child, Preschool ; Female ; Humans ; Infant ; Infant, Newborn ; Male |
| مستخلص: | Genetic aberrations in leukemia often lead to the formation of expressed chimeric genes, which should be assessed for proper diagnosis and therapy. Modern methods of molecular diagnostic mainly allow to identify already known fusion genes. RNAseq is an efficient tool for identification of rare and novel chimeric transcripts. Here we present the results of the whole transcriptome analysis of bone marrow samples from five patients with acute myeloblastic leukemia and one, with myelodysplastic syndrome. The whole-transcriptome analysis was performed using Illumina/Solexa approach. We found rare or unknown chimeric transcripts including ETV6-MDS1, MN1-ETV6, OAZ1-PTMA, and MLLT10-GRIA4. Each of these transcripts was confirmed by RT-PCR and Sanger sequencing. |
| فهرسة مساهمة: | Keywords: acute leukemia; fusion genes; massive parallel sequencing; whole-transcriptome analysis |
| المشرفين على المادة: | 0 (Oncogene Proteins, Fusion) 0 (RNA, Messenger) 0 (RNA, Neoplasm) |
| تواريخ الأحداث: | Date Created: 20180427 Date Completed: 20181211 Latest Revision: 20181211 |
| رمز التحديث: | 20231215 |
| DOI: | 10.7868/S0026898418020064 |
| PMID: | 29695691 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0026-8984 |
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| DOI: | 10.7868/S0026898418020064 |