دورية أكاديمية
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High-throughput sequencing reveals a novel closterovirus in arracacha (Arracacia xanthorrhiza).

التفاصيل البيبلوغرافية
العنوان: High-throughput sequencing reveals a novel closterovirus in arracacha (Arracacia xanthorrhiza).
المؤلفون: Orílio AF; Department of Cell Biology, University of Brasilia, Campus Universitário Darcy Ribeiro, Brasília, Distrito Federal, 70910-900, Brazil. aneliseorilio@gmail.com., Blawid R; Department of Cell Biology, University of Brasilia, Campus Universitário Darcy Ribeiro, Brasília, Distrito Federal, 70910-900, Brazil., Costa GA; Department of Cell Biology, University of Brasilia, Campus Universitário Darcy Ribeiro, Brasília, Distrito Federal, 70910-900, Brazil., Gomes SSVSF; Department of Cell Biology, University of Brasilia, Campus Universitário Darcy Ribeiro, Brasília, Distrito Federal, 70910-900, Brazil., Nagata T; Department of Cell Biology, University of Brasilia, Campus Universitário Darcy Ribeiro, Brasília, Distrito Federal, 70910-900, Brazil., Madeira NR; Embrapa Vegetables, Caixa Postal 218, Brasília, DF, 70275-970, Brazil., Inoue-Nagata AK; Embrapa Vegetables, Caixa Postal 218, Brasília, DF, 70275-970, Brazil., Resende RO; Department of Cell Biology, University of Brasilia, Campus Universitário Darcy Ribeiro, Brasília, Distrito Federal, 70910-900, Brazil.
المصدر: Archives of virology [Arch Virol] 2018 Sep; Vol. 163 (9), pp. 2547-2550. Date of Electronic Publication: 2018 May 07.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer-Verlag Country of Publication: Austria NLM ID: 7506870 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-8798 (Electronic) Linking ISSN: 03048608 NLM ISO Abbreviation: Arch Virol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Wien, New York, Springer-Verlag.
مواضيع طبية MeSH: Apiaceae/*virology , Closterovirus/*isolation & purification , Plant Diseases/*virology, Brazil ; Closterovirus/classification ; Closterovirus/genetics ; Genome, Viral ; High-Throughput Nucleotide Sequencing ; Open Reading Frames ; Phylogeny ; Viral Proteins/genetics
مستخلص: High-throughput sequencing analysis detected a clostero-like virus from arracacha plants (Arracacia xanthorrhiza) in Brazil. The complete genome sequence, confirmed by RACE and Sanger sequencing, consists of 15,763 nucleotides with nine predicted open reading frames (ORFs) in a typical closterovirus genome organisation. The putative RNA-dependent RNA polymerase (RdRp), heat shock protein 70 homologue (Hsp70h), and coat protein showed 55-65, 38-44, and 20-36% amino acid sequence identity, respectively, to the homologous proteins of known closteroviruses. Phylogenetic analysis of Hsp70h showed that this putative novel arracacha plant virus was related to members of the genus Closterovirus in the family Closteroviridae. These results suggest that this virus, tentatively named "arracacha virus 1" (AV-1), is a novel member of the genus Closterovirus. This is the first closterovirus identified in arracacha plants.
المشرفين على المادة: 0 (Viral Proteins)
تواريخ الأحداث: Date Created: 20180509 Date Completed: 20190321 Latest Revision: 20190321
رمز التحديث: 20240628
DOI: 10.1007/s00705-018-3863-1
PMID: 29736672
قاعدة البيانات: MEDLINE
الوصف
تدمد:1432-8798
DOI:10.1007/s00705-018-3863-1