دورية أكاديمية
Pleiotropic effects of hemagglutinin amino acid substitutions of influenza A(H1N1)pdm09 virus escape mutants.
| العنوان: | Pleiotropic effects of hemagglutinin amino acid substitutions of influenza A(H1N1)pdm09 virus escape mutants. |
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| المؤلفون: | Rudneva IA; D.I. Ivanovsky Instutute of Virology FSBI 'N.F.Gamaleya NRCEM', Ministry of Health of Russian Federation, 123098, Moscow, Russian Federation., Timofeeva TA; D.I. Ivanovsky Instutute of Virology FSBI 'N.F.Gamaleya NRCEM', Ministry of Health of Russian Federation, 123098, Moscow, Russian Federation. Electronic address: timofeeva.tatyana@inbox.ru., Mukasheva EA; D.I. Ivanovsky Instutute of Virology FSBI 'N.F.Gamaleya NRCEM', Ministry of Health of Russian Federation, 123098, Moscow, Russian Federation., Ignatieva AV; D.I. Ivanovsky Instutute of Virology FSBI 'N.F.Gamaleya NRCEM', Ministry of Health of Russian Federation, 123098, Moscow, Russian Federation., Shilov AA; D.I. Ivanovsky Instutute of Virology FSBI 'N.F.Gamaleya NRCEM', Ministry of Health of Russian Federation, 123098, Moscow, Russian Federation., Burtseva EI; D.I. Ivanovsky Instutute of Virology FSBI 'N.F.Gamaleya NRCEM', Ministry of Health of Russian Federation, 123098, Moscow, Russian Federation., Timofeev BI; D.I. Ivanovsky Instutute of Virology FSBI 'N.F.Gamaleya NRCEM', Ministry of Health of Russian Federation, 123098, Moscow, Russian Federation., Kaverin NV; D.I. Ivanovsky Instutute of Virology FSBI 'N.F.Gamaleya NRCEM', Ministry of Health of Russian Federation, 123098, Moscow, Russian Federation. |
| المصدر: | Virus research [Virus Res] 2018 Jun 02; Vol. 251, pp. 91-97. Date of Electronic Publication: 2018 May 16. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8410979 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7492 (Electronic) Linking ISSN: 01681702 NLM ISO Abbreviation: Virus Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier Science, c1984- |
| مواضيع طبية MeSH: | Amino Acid Substitution* , Immune Evasion* , Mutation, Missense*, Hemagglutinin Glycoproteins, Influenza Virus/*genetics , Hemagglutinin Glycoproteins, Influenza Virus/*metabolism , Influenza A Virus, H1N1 Subtype/*genetics , Influenza A Virus, H1N1 Subtype/*physiology, Animals ; Antibodies, Viral/immunology ; Chick Embryo ; Hemagglutinin Glycoproteins, Influenza Virus/chemistry ; Hemagglutinin Glycoproteins, Influenza Virus/immunology ; Hydrogen-Ion Concentration ; Influenza A Virus, H1N1 Subtype/immunology ; Mice ; Protein Stability ; Temperature ; Virus Internalization/drug effects ; Virus Internalization/radiation effects ; Virus Replication/drug effects ; Virus Replication/radiation effects |
| مستخلص: | In the present study we assessed pleiotropic characteristics of the antibody-selected mutations. We investigated pH optimum of fusion, temperatures of HA heat inactivation, in vivo and in vitro replication kinetics, and connectivity with panel of sera of survivors patients in different epidemic seasons of the previously obtained influenza H1 escape mutants. Our results showed that N133D (H3 numbering) mutation significantly lowered the pH of fusion optimum. Several amino acid substitutions, including K163 N, Q192 L, D190E, G228E, and K285 M, reduced the stability of HA as determined by heat inactivation, whereas A198E substitution is associated with significant increase in HA thermostability compared to the wild-type virus. We found that amino acid change D190 N was associated with a significant decrease in viral growth in eggs and mice. Our potential antigenic variants, except readapted variant, which contained A198E mutation, did not reach fixation in infected people. Overall, a co-variation between antigenic specificity and different HA phenotypic properties was demonstrated. (Copyright © 2018 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Escape mutants; H1 hemagglutinin; Pandemic influenza virus; Pleiotropic antibody-neutralizing mutations |
| المشرفين على المادة: | 0 (Antibodies, Viral) 0 (H1N1 virus hemagglutinin) 0 (Hemagglutinin Glycoproteins, Influenza Virus) |
| تواريخ الأحداث: | Date Created: 20180514 Date Completed: 20180921 Latest Revision: 20180921 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.virusres.2018.05.002 |
| PMID: | 29753891 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7492 |
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| DOI: | 10.1016/j.virusres.2018.05.002 |