دورية أكاديمية
أعرض في EDS

Effect of Heat Stress on Sperm DNA: Protamine Assessment in Ram Spermatozoa and Testicle.

التفاصيل البيبلوغرافية
العنوان: Effect of Heat Stress on Sperm DNA: Protamine Assessment in Ram Spermatozoa and Testicle.
المؤلفون: Hamilton TRDS; Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, SP, Brazil., Siqueira AFP; Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, SP, Brazil., de Castro LS; Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, SP, Brazil., Mendes CM; Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, SP, Brazil., Delgado JC; Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, SP, Brazil., de Assis PM; Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, SP, Brazil., Mesquita LP; Department of Animal Pathology, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, SP, Brazil., Maiorka PC; Department of Animal Pathology, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, SP, Brazil., Nichi M; Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, SP, Brazil., Goissis MD; Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, SP, Brazil., Visintin JA; Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, SP, Brazil., Assumpção MEODÁ; Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, 05508 270, Cidade Universitária, Sao Paulo, SP, Brazil.
المصدر: Oxidative medicine and cellular longevity [Oxid Med Cell Longev] 2018 Mar 25; Vol. 2018, pp. 5413056. Date of Electronic Publication: 2018 Mar 25 (Print Publication: 2018).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Hindawi Pub. Corp Country of Publication: United States NLM ID: 101479826 Publication Model: eCollection Cited Medium: Internet ISSN: 1942-0994 (Electronic) Linking ISSN: 19420994 NLM ISO Abbreviation: Oxid Med Cell Longev Subsets: MEDLINE
أسماء مطبوعة: Publication: 2011- : New York : Hindawi Pub. Corp.
Original Publication: 2008-2010: Austin, TX : Landes Bioscience
مواضيع طبية MeSH: DNA/*drug effects , Sperm Motility/*drug effects , Spermatozoa/*physiology , Testis/*physiology, Animals ; Male ; Protamines
مستخلص: Sperm DNA fragmentation is considered one of the main causes of male infertility. The most accepted causes of sperm DNA damage are deleterious actions of reactive oxygen species (ROS), defects in protamination, and apoptosis. Ram sperm are highly prone to those damages due to the high susceptibility to ROS and to oxidative stress caused by heat stress. We aimed to evaluate the effects of heat stress on the chromatin of ejaculated and epididymal sperm and the activation of apoptotic pathways in different cell types in ram testis. We observed higher percentages of ejaculated sperm with increased chromatin fragmentation in the heat stress group; a fact that was unexpectedly not observed in epididymal sperm. Heat stress group presented a higher percentage of spermatozoa with DNA fragmentation and increased number of mRNA copies of transitional protein 1. Epididymal sperm presented greater gene expression of protamine 1 on the 30th day of the spermatic cycle; however, no differences in protamine protein levels were observed in ejaculated sperm and testis. Localization of proapoptotic protein BAX or BCL2 in testis was not different. In conclusion, testicular heat stress increases ram sperm DNA fragmentation without changes in protamination and apoptotic patterns.
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المشرفين على المادة: 0 (Protamines)
9007-49-2 (DNA)
تواريخ الأحداث: Date Created: 20180517 Date Completed: 20181005 Latest Revision: 20181114
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC5889875
DOI: 10.1155/2018/5413056
PMID: 29765499
قاعدة البيانات: MEDLINE
الوصف
تدمد:1942-0994
DOI:10.1155/2018/5413056