دورية أكاديمية
MiR-299-5p targets cell cycle to promote cell proliferation and progression of osteosarcoma.
| العنوان: | MiR-299-5p targets cell cycle to promote cell proliferation and progression of osteosarcoma. |
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| المؤلفون: | Zhang CL; Department of Orthopedics, the Second Affiliated Hospital of Soochow University, Suzhou, China. dqr@szgk.net., Li LB, She C, Xie Y, Ge DW, Dong QR |
| المصدر: | European review for medical and pharmacological sciences [Eur Rev Med Pharmacol Sci] 2018 May; Vol. 22 (9), pp. 2606-2613. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Verduci Country of Publication: Italy NLM ID: 9717360 Publication Model: Print Cited Medium: Internet ISSN: 2284-0729 (Electronic) Linking ISSN: 11283602 NLM ISO Abbreviation: Eur Rev Med Pharmacol Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Rome : Verduci, [1997- |
| مواضيع طبية MeSH: | Cell Cycle* , Cell Proliferation*, Bone Neoplasms/*metabolism , MicroRNAs/*metabolism , Osteosarcoma/*metabolism, Bone Neoplasms/genetics ; Bone Neoplasms/mortality ; Bone Neoplasms/pathology ; Cell Line, Tumor ; Cyclin-Dependent Kinases/genetics ; Cyclin-Dependent Kinases/metabolism ; Cyclins/genetics ; Cyclins/metabolism ; Databases, Genetic ; Disease Progression ; Gene Expression Regulation, Neoplastic ; Humans ; MicroRNAs/genetics ; Osteosarcoma/genetics ; Osteosarcoma/mortality ; Osteosarcoma/pathology ; Signal Transduction |
| مستخلص: | Objective: Osteosarcoma is a common primary bone tumor with high mortality. MicroRNA (miRNA, miR) is a small RNA with 20-25 nucleotides, which could regulate diverse biological processes by targeting 3'-UTR of gene to degrade it. MiR-299-5p has been reported to participate in the progression of many diseases, but the role in osteosarcoma is still uncertain. The aim of this work was to investigate the expression of miR-299-5p in osteosarcoma and its clinical significance. Materials and Methods: The datasets of osteosarcoma miRNA was searched in Gene Expression Omnibus (GEO) datasets, including GSE65071, GSE39040, and GSE39055. Osteosarcoma U2 and MG-63 cells were cultured in our study. Cell proliferation level after transfection was detected by using Cell Counting Kit-8 (CCK8) and colon formation assay. Cell cycles were explored using flow cytometer and cell protein expression levels after that the transfection was detected by Western blotting. Results: We found that ROC curve analysis showed that miR-299-5p was a sensitivity diagnostic criteria and GSEA indicated that miRNA-299-5p may regulate cell cycle. Gain of function assay showed that miR-299-5p promotes cell cycle transition and proliferation. Reversely, the opposite results were observed with loss of function assay. At last, Western blotting assay showed that miR-299-5p may promote cell cycle transition by regulating CDK family. |
| المشرفين على المادة: | 0 (Cyclins) 0 (MIR4299 microRNA, human) 0 (MicroRNAs) EC 2.7.11.22 (Cyclin-Dependent Kinases) |
| تواريخ الأحداث: | Date Created: 20180518 Date Completed: 20191212 Latest Revision: 20191217 |
| رمز التحديث: | 20231215 |
| DOI: | 10.26355/eurrev_201805_14954 |
| PMID: | 29771409 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2284-0729 |
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| DOI: | 10.26355/eurrev_201805_14954 |