دورية أكاديمية
Polypyrrole increases branching and neurite extension by Neuro2A cells on PBAT ultrathin fibers.
| العنوان: | Polypyrrole increases branching and neurite extension by Neuro2A cells on PBAT ultrathin fibers. |
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| المؤلفون: | Granato AEC; Department of Biochemistry, Neurobiology Lab, Escola Paulista de Medicina, Universidade Federal São Paulo, São Paulo., Ribeiro AC; Institute of Science and Technology, Universidade Brasil, São Paulo, SP, Brazil., Marciano FR; Institute of Science and Technology, Universidade Brasil, São Paulo, SP, Brazil; Department of Chemical Engineering, Northeastern University, Boston, MA, USA., Rodrigues BVM; Institute of Science and Technology, Universidade Brasil, São Paulo, SP, Brazil; Plasma and Processes Laboratory, Instituto Tecnológico de Aeronáutica, São Jose dos Campos, SP, Brazil., Lobo AO; Institute of Science and Technology, Universidade Brasil, São Paulo, SP, Brazil; Interdisciplinary Laboratory for Advanced Materials, Materials Science and Engineering graduation program, Technology Center, Universidade Federal do Piauí, Teresina, PI, Brazil; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA. Electronic address: aolobo@pq.cnpq.br., Porcionatto M; Department of Biochemistry, Neurobiology Lab, Escola Paulista de Medicina, Universidade Federal São Paulo, São Paulo. Electronic address: marimelia.porcionatto@unifesp.br. |
| المصدر: | Nanomedicine : nanotechnology, biology, and medicine [Nanomedicine] 2018 Aug; Vol. 14 (6), pp. 1753-1763. Date of Electronic Publication: 2018 May 18. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 101233142 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1549-9642 (Electronic) Linking ISSN: 15499634 NLM ISO Abbreviation: Nanomedicine Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, NY : Elsevier, c2005- |
| مواضيع طبية MeSH: | Mesenchymal Stem Cells/*pathology , Nanofibers/*chemistry , Neurites/*pathology , Neuroblastoma/*pathology , Polyesters/*administration & dosage , Polymers/*administration & dosage , Pyrroles/*administration & dosage, Animals ; Cell Adhesion/drug effects ; Cell Differentiation/drug effects ; Cell Proliferation/drug effects ; Mesenchymal Stem Cells/drug effects ; Mice ; Mice, Inbred C57BL ; Neurites/drug effects ; Neuroblastoma/drug therapy ; Polyesters/chemistry ; Polymers/chemistry ; Pyrroles/chemistry ; Tissue Scaffolds ; Tumor Cells, Cultured |
| مستخلص: | We present a methodology for production and application of electrospun hybrid materials containing commercial polyester (poly (butylene adipate-co-terephthalate; PBAT), and a conductive polymer (polypyrrole; PPy) as scaffold for neuronal growth and differentiation. The physical-chemical properties of the scaffolds and optimization of the electrospinning parameters are presented. The electrospun scaffolds are biocompatible and allow proper adhesion and spread of mesenchymal stem cells (MSCs). Fibers produced with PBAT with or without PPy were used as scaffold for Neuro2a mouse neuroblastoma cells adhesion and differentiation. Neuro2a adhered to PBAT and PBAT/PPy2% scaffolds without laminin coating. However, Neuro2a failed to differentiate in PBAT when stimulated by treatment with retinoic acid (RA), but differentiated in PBAT/PPy2% fibers. We hypothesize that PBAT hydrophobicity inhibited proper spreading and further differentiation, and inhibition was overcome by coating the PBAT fibers with laminin. We conclude that fibers produced with the combination of PBAT and PPy can support neuronal differentiation. (Copyright © 2018 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Electrospinning; Mesenchymal stem cells; Neuronal differentiation; PBAT/PPy |
| المشرفين على المادة: | 0 (Polyesters) 0 (Polymers) 0 (Pyrroles) 0 (poly(butylene adipate-co-butylene terephthalate)) 30604-81-0 (polypyrrole) |
| تواريخ الأحداث: | Date Created: 20180521 Date Completed: 20190111 Latest Revision: 20190111 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.nano.2018.05.004 |
| PMID: | 29778889 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1549-9642 |
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| DOI: | 10.1016/j.nano.2018.05.004 |