دورية أكاديمية
UPLC-ESI-QTOF-MS 2 characterisation of Cola nitida resin fractions with inhibitory effects on NO and TNF-α released by LPS-activated J774 macrophage and on Trypanosoma cruzi and Leishmania amazonensis.
| العنوان: | UPLC-ESI-QTOF-MS 2 characterisation of Cola nitida resin fractions with inhibitory effects on NO and TNF-α released by LPS-activated J774 macrophage and on Trypanosoma cruzi and Leishmania amazonensis. |
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| المؤلفون: | Frankenberger L; Department of Pharmaceutical Sciences, CCS, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil., D Mora T; Department of Clinical Analysis, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil., de Siqueira CD; Department of Clinical Analysis, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil., Filippin-Monteiro FB; Department of Clinical Analysis, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil., de Moraes MH; Department of Microbiology, Immunology and Parasitology, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil., Biavatti MW; Department of Pharmaceutical Sciences, CCS, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil., Steindel M; Department of Microbiology, Immunology and Parasitology, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil., Sandjo LP; Department of Pharmaceutical Sciences, CCS, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil. |
| المصدر: | Phytochemical analysis : PCA [Phytochem Anal] 2018 Nov; Vol. 29 (6), pp. 577-589. Date of Electronic Publication: 2018 May 29. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley Country of Publication: England NLM ID: 9200492 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1099-1565 (Electronic) Linking ISSN: 09580344 NLM ISO Abbreviation: Phytochem Anal Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Chichester, Sussex, UK : Wiley, c1990- |
| مواضيع طبية MeSH: | Chromatography, Liquid/*methods , Cola/*chemistry , Leishmania/*drug effects , Macrophages/*drug effects , Mass Spectrometry/*methods , Trypanosoma cruzi/*drug effects, Animals ; Cell Line ; Gene Expression Regulation/drug effects ; Interleukin-1beta/genetics ; Interleukin-1beta/metabolism ; Interleukin-6/genetics ; Interleukin-6/metabolism ; Lipopolysaccharides/pharmacology ; Mice ; Nitric Oxide/metabolism ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Resins, Plant/chemistry ; Tumor Necrosis Factor-alpha/metabolism |
| مستخلص: | Introduction: The resin of Cola nitida is used in western Cameroon as incense for spiritual protection and during ritual ceremonies. This plant secretion has never been investigated although previous chemical and biological studies on other resins have drawn many attentions. Objective: The resin fractions which revealed inhibitory effect on nitric oxide (NO) and tumour necrosis factor alpha (TNF-α) released by lipopolysaccharide (LPS)-activated J774 macrophage as well as on intracellular forms of Leishmania amazonensis and Trypanosoma cruzi amastigote were chemically characterised. Moreover, their antiparasitic activities were compared to those of semi-synthetic triterpenes. Methodology: The anti-inflammatory activity was evaluated by measuring the nitrite production and the TNF-α concentration in the supernatants of LPS-activated macrophages by antigen capture enzyme-linked immunosorbent assay. Moreover, the antiparasitic assay was performed by infecting the host cells (THP-1) in a ratio parasite/cell 10:1 (L. amazonensis) and 2:1 (T. cruzi) and then exposed to the samples. The resin was separated in vacuo by liquid chromatography because of its sticky behaviour and the chemical profiles of the obtained fractions (F1-F4) were established by dereplication based on UPLC-ESI-MS 2 data while semi-synthetic triterpenes were prepared from α-amyrin by oxidation reactions. Results: Fractions F1-F4 inhibited NO and TNF-α almost similarly. However, only F1, F3 and F4 showed promising antiparasitic activities while F2 was moderately active against both parasites. Hence, F1-F4 were exclusively composed of pentacyclic triterpenes bearing oleanane and ursane skeletons. Semi-synthetic compounds revealed no to moderate antiparasitic activity compared to the fractions. Conclusion: Although it will be difficult to prove the interaction resin-spirit, interesting bioactivities were found in the resin fractions. (Copyright © 2018 John Wiley & Sons, Ltd.) |
| معلومات مُعتمدة: | CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior); CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) |
| فهرسة مساهمة: | Keywords: Cola nitida resin; NO and TNF-α inhibition; UPLC-ESI-QTOF-MS2; antiprotozoal activity; pentacyclic triterpenes |
| المشرفين على المادة: | 0 (Interleukin-1beta) 0 (Interleukin-6) 0 (Lipopolysaccharides) 0 (Plant Extracts) 0 (Resins, Plant) 0 (Tumor Necrosis Factor-alpha) 31C4KY9ESH (Nitric Oxide) |
| تواريخ الأحداث: | Date Created: 20180530 Date Completed: 20181025 Latest Revision: 20181025 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1002/pca.2771 |
| PMID: | 29808594 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1099-1565 |
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| DOI: | 10.1002/pca.2771 |