دورية أكاديمية
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NADP-Malate Dehydrogenase of Sweet Sorghum Improves Salt Tolerance of Arabidopsis thaliana.

التفاصيل البيبلوغرافية
العنوان: NADP-Malate Dehydrogenase of Sweet Sorghum Improves Salt Tolerance of Arabidopsis thaliana.
المؤلفون: Guo Y; Shandong Provincial Key Laboratory of Plant Stress, College of Life Science , Shandong Normal University , Jinan 250014 , China., Song Y; Shandong Provincial Key Laboratory of Plant Stress, College of Life Science , Shandong Normal University , Jinan 250014 , China., Zheng H; Shandong Provincial Key Laboratory of Plant Stress, College of Life Science , Shandong Normal University , Jinan 250014 , China., Zhang Y; Shandong Provincial Key Laboratory of Plant Stress, College of Life Science , Shandong Normal University , Jinan 250014 , China., Guo J; Shandong Provincial Key Laboratory of Plant Stress, College of Life Science , Shandong Normal University , Jinan 250014 , China., Sui N; Shandong Provincial Key Laboratory of Plant Stress, College of Life Science , Shandong Normal University , Jinan 250014 , China.
المصدر: Journal of agricultural and food chemistry [J Agric Food Chem] 2018 Jun 20; Vol. 66 (24), pp. 5992-6002. Date of Electronic Publication: 2018 Jun 08.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, American Chemical Society.
مواضيع طبية MeSH: Arabidopsis/*physiology , Malate Dehydrogenase (NADP+)/*metabolism , Plant Proteins/*metabolism , Plants, Genetically Modified/*physiology , Sorghum/*enzymology, Arabidopsis/genetics ; Arabidopsis/growth & development ; Chlorophyll/metabolism ; Gene Expression Regulation, Plant ; Malate Dehydrogenase (NADP+)/genetics ; Photosynthesis ; Plant Proteins/genetics ; Plants, Genetically Modified/genetics ; Plants, Genetically Modified/growth & development ; Salt Tolerance ; Seedlings/genetics ; Seedlings/physiology ; Sodium Chloride/metabolism ; Sorghum/genetics ; Stress, Physiological
مستخلص: Sweet sorghum is a C 4 crop that shows high salt tolerance and high yield. NADP-malate dehydrogenase ( NADP-ME) is a crucial enzyme of the C 4 pathway. The regulatory mechanism of NADP-ME remains unclear. In this study, we isolated SbNADP-ME from sweet sorghum. The open reading frame of SbNADP-ME is 1911 bp and 637 amino acid residues. Quantitative real-time PCR analysis showed that SbNADP-ME transcription in sweet sorghum was enhanced by salt stress. The SbNADP-ME transcript level was highest under exposure to 150 mM NaCl. Arabidopsis overexpressing SbNADP-ME showed increased germination rate and root length under NaCl treatments. At the seedling stage, physiological photosynthesis parameters, chlorophyll content, PSII photochemical efficiency, and PSI oxidoreductive activity in the wild type decreased more severely than in the overexpression lines but less than in T-DNA insertion mutants under salt stress. Overexpression of SbNADP-ME in Arabidopsis may also increase osmotic adjustment and scavenging activity on DPPH and decrease membrane peroxidation. These results suggest that SbNADP-ME overexpression in Arabidopsis increases salt tolerance and alleviates PSII and PSI photoinhibition under salt stress by improving photosynthetic capacity.
فهرسة مساهمة: Keywords: SbNADP-ME; photosynthesis; salt stress; sweet sorghum
المشرفين على المادة: 0 (Plant Proteins)
1406-65-1 (Chlorophyll)
451W47IQ8X (Sodium Chloride)
EC 1.1.1.82 (Malate Dehydrogenase (NADP+))
تواريخ الأحداث: Date Created: 20180531 Date Completed: 20180717 Latest Revision: 20181202
رمز التحديث: 20221213
DOI: 10.1021/acs.jafc.8b02159
PMID: 29847118
قاعدة البيانات: MEDLINE
الوصف
تدمد:1520-5118
DOI:10.1021/acs.jafc.8b02159