دورية أكاديمية
[Possible role of the T antigen in inducing chromosome aberrations in SV40 virus-transformed cells].
| العنوان: | [Possible role of the T antigen in inducing chromosome aberrations in SV40 virus-transformed cells]. |
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| عنوان ترانسليتريتد: | Vozmozhnaia rol' T-antigena v induktsii khromosomnykh aberratsiĭ v kletkakh, transformirovannykh virusom SV40. |
| المؤلفون: | Drize OB, Sokova OI, Nikashina EB, Shliankevich MA, Shapot VS |
| المصدر: | Tsitologiia [Tsitologiia] 1985 Jan; Vol. 27 (1), pp. 76-82. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Rossiiskaia Akademiia Nauk Country of Publication: Russia (Federation) NLM ID: 0417363 Publication Model: Print Cited Medium: Print ISSN: 0041-3771 (Print) Linking ISSN: 00413771 NLM ISO Abbreviation: Tsitologiia Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Moskva : Rossiiskaia Akademiia Nauk Original Publication: Moskva, Akademiia nauk SSSR. |
| مواضيع طبية MeSH: | Cell Transformation, Viral* , Chromosome Aberrations*, Antigens, Viral, Tumor/*genetics , Simian virus 40/*pathogenicity, Animals ; Cell Transformation, Neoplastic ; Cells, Cultured ; Cricetinae ; Mesocricetus ; Mutation ; Temperature ; Time Factors |
| مستخلص: | A possible role of the simian virus 40 T antigen in chromosome damages in transformed cells was examined. Two lines of Golden hamster embryonal fibroblasts, transformed by SV40 tsA30 and ts239 mutants (He30 and He239, respectively), were incubated at nonpermissive (40.5-41 degrees C) or permissive (33 degrees C) temperatures. Chromosome aberrations were registered in either subline after 3, 6, 9 and 12 weeks of cultivation under the above conditions. In the both cell lines kept at 33 degrees the frequency of aberrant metaphases and the number of chromosome breaks per cell increased drastically by week 3 of cultivation, and such a state was preserved up to week 12. The frequency of aberrant metaphases in cells cultivated at 41 degrees was maintained at the constant level (He239) or at slightly higher than that in the original culture (He30). The sublines He239, originally incubated at 33 or 40.5 degrees, were then shifted to 40.5 and 33 degrees, respectively. As a result the number of chromosome aberrations either decreased (33----40.5 degrees) or increased (40.5----33 degrees) as early as on day 2, and these patterns were stabilized at the level corresponding to the new conditions. We assayed the induction of DNA breaks in cells, grown at the permissive or nonpermissive temperatures, by using DNA sedimentation in the alkaline sucrose gradient. The DNA sedimentation peaks of cells cultured at 37 and 41 degrees coincided, whereas the DNA of cells cultured at 33 degrees was represented by shorter fragments. |
| المشرفين على المادة: | 0 (Antigens, Viral, Tumor) |
| تواريخ الأحداث: | Date Created: 19850101 Date Completed: 19850508 Latest Revision: 20061115 |
| رمز التحديث: | 20221208 |
| PMID: | 2984814 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0041-3771 |
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