دورية أكاديمية
Detection and quantification of hepatitis E virus in the absence of IgG and IgM anti-HEV in HIV-positive patients.
| العنوان: | Detection and quantification of hepatitis E virus in the absence of IgG and IgM anti-HEV in HIV-positive patients. |
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| المؤلفون: | Salvio AL; Laboratory of Molecular Virology, Oswaldo Cruz Institute/Fiocruz, Rio de Janeiro, Brazil., Lopes AO; Laboratory of Molecular Virology, Oswaldo Cruz Institute/Fiocruz, Rio de Janeiro, Brazil., Almeida AJ; Hematology Department, Gaffrée & Guinle Universitary Hospital, Rio de Janeiro State Federal University/UniRio, Rio de Janeiro, Brazil., Gardinali NR; Laboratory of Technological Development in Virology, Oswaldo Cruz Institute/Fiocruz, Rio de Janeiro, Brazil., Lima LRP; Laboratory of Molecular Virology, Oswaldo Cruz Institute/Fiocruz, Rio de Janeiro, Brazil., de Oliveira JM; Laboratory of Technological Development in Virology, Oswaldo Cruz Institute/Fiocruz, Rio de Janeiro, Brazil., Sion FS; Hematology Department, Gaffrée & Guinle Universitary Hospital, Rio de Janeiro State Federal University/UniRio, Rio de Janeiro, Brazil., Ribeiro LCP; Hematology Department, Gaffrée & Guinle Universitary Hospital, Rio de Janeiro State Federal University/UniRio, Rio de Janeiro, Brazil., Pinto MA; Laboratory of Technological Development in Virology, Oswaldo Cruz Institute/Fiocruz, Rio de Janeiro, Brazil., de Paula VS; Laboratory of Molecular Virology, Oswaldo Cruz Institute/Fiocruz, Rio de Janeiro, Brazil. |
| المصدر: | Journal of applied microbiology [J Appl Microbiol] 2018 Oct; Vol. 125 (4), pp. 1208-1215. Date of Electronic Publication: 2018 Jul 30. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 9706280 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2672 (Electronic) Linking ISSN: 13645072 NLM ISO Abbreviation: J Appl Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2022- : Oxford : Oxford University Press Original Publication: Oxford : Published for the Society for Applied Bacteriology by Blackwell Science, c1997- |
| مواضيع طبية MeSH: | HIV Infections/*blood , Hepatitis Antibodies/*blood , Hepatitis E/*virology , Hepatitis E virus/*isolation & purification , Immunoglobulin G/*blood , Immunoglobulin M/*blood, Adult ; Brazil ; Coinfection/blood ; Coinfection/virology ; Female ; Genotype ; HIV Infections/virology ; Hepatitis E/blood ; Hepatitis E virus/genetics ; Hepatitis E virus/immunology ; Hepatitis E virus/physiology ; Humans ; Male ; Middle Aged ; Real-Time Polymerase Chain Reaction ; Viral Load |
| مستخلص: | Aims: To improve RT-qPCR with an internal control and a synthetic standard curve to detect HEV in HIV co-infected patients. Methods and Results: A single-stranded RNA (ssRNA) and a double-stranded DNA (dsDNA) synthetic curve were designed, compared to the international reference panel for HEV genotypes, and tested to quantify and detect a reference panel for HEV genotypes. The detection limit of the RNA synthetic curve (50 copies per ml) was better than the DNA synthetic curve (100 copies per ml) and the WHO standard curve (250 copies per ml). Then, 280 serum samples from HIV-positive patients were tested for HEV RNA, which was detected in 3·6% of serum samples. The viral load ranged from 2 × 10 2 copies per ml to 4·78 × 10 8 copies per ml. HEV IgM/IgG antibodies were not detected in the RNA-positive patients. Sequencing analysis of HEV showed that the virus belongs to genotype 3 (HEV GT3). Conclusions: Real-time PCR was a useful tool to estimate co-infection with HEV/HIV, even in patients with low viral loads and undetectable anti-HEV IgG and IgM antibodies. Significance and Impact of the Study: Hepatitis E virus genotype 3 (HEV GT3) has been associated with silent chronic hepatitis and cirrhosis in HIV-positive subjects worldwide, but there is a lack of data on this co-infection in Brazil. (© 2018 The Society for Applied Microbiology.) |
| فهرسة مساهمة: | Keywords: HIV; coinfection; diagnosis; hepatitis E; real-time PCR; standard curve; viral load |
| المشرفين على المادة: | 0 (Hepatitis Antibodies) 0 (Immunoglobulin G) 0 (Immunoglobulin M) |
| تواريخ الأحداث: | Date Created: 20180620 Date Completed: 20181126 Latest Revision: 20220409 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1111/jam.14024 |
| PMID: | 29920871 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1365-2672 |
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| DOI: | 10.1111/jam.14024 |