دورية أكاديمية
[The Effect of recombinant adiponectin on apoptosis induced by t-BHP in human umbilical vein endothelial cells].
| العنوان: | [The Effect of recombinant adiponectin on apoptosis induced by t-BHP in human umbilical vein endothelial cells]. |
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| المؤلفون: | Wu L; Department of Endocrinology, Fujian Medical University Union Hospital, Fujian Institute of Endocrinology, Fujian Medical University, Fuzhou 350001, China., Liu LY; Department of Endocrinology, Fujian Medical University Union Hospital, Fujian Institute of Endocrinology, Fujian Medical University, Fuzhou 350001, China., Guan BB; Department of Endocrinology, Fujian Medical University Union Hospital, Fujian Institute of Endocrinology, Fujian Medical University, Fuzhou 350001, China., Wang LX; Department of Endocrinology, Fujian Medical University Union Hospital, Fujian Institute of Endocrinology, Fujian Medical University, Fuzhou 350001, China.; Department of Geriatrics, Fujian Medical University Union Hospital, Fujian Medical University, Fuzhou 350001, China., Liu XY; Department of Geriatrics, Fujian Medical University Union Hospital, Fujian Medical University, Fuzhou 350001, China., Chen M; Department of Endocrinology, Fujian Medical University Union Hospital, Fujian Institute of Endocrinology, Fujian Medical University, Fuzhou 350001, China., Chen Z; College of Pharmacy, Fujian Medical University, Fujian Medical University, Fuzhou 350001, China., Liu LB; Department of Endocrinology, Fujian Medical University Union Hospital, Fujian Institute of Endocrinology, Fujian Medical University, Fuzhou 350001, China.; Institute of the Elderly Health Sciences, Fujian Medical University, Fuzhou 350001, China. |
| المصدر: | Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology [Zhongguo Ying Yong Sheng Li Xue Za Zhi] 2016 Jun 08; Vol. 32 (6), pp. 540-544. |
| نوع المنشور: | Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Zhongguo ying yong sheng li xue za zhi bian ji bu Country of Publication: China NLM ID: 9426407 Publication Model: Print Cited Medium: Print ISSN: 1000-6834 (Print) Linking ISSN: 10006834 NLM ISO Abbreviation: Zhongguo Ying Yong Sheng Li Xue Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Tianjin : Zhongguo ying yong sheng li xue za zhi bian ji bu Original Publication: Beijing Shi : Zhongguo ying yong sheng li xue za zhi bian ji bu, |
| مواضيع طبية MeSH: | Apoptosis*, Adiponectin/*pharmacology , Human Umbilical Vein Endothelial Cells/*drug effects, Caspase 3/metabolism ; Cell Survival ; Cells, Cultured ; Human Umbilical Vein Endothelial Cells/cytology ; Humans ; JNK Mitogen-Activated Protein Kinases/metabolism ; Recombinant Proteins/pharmacology ; tert-Butylhydroperoxide/adverse effects |
| مستخلص: | Objective: To investigate the protective effect and possible mechanism of recombinant adiponectin on apoptosis in Human Umbilical Vein Endothelial Cells (HUVECs) induced by tert-butyl hydroperoxide (t-BHP). Methods: HUVECs were cultured in vitro and apoptosis was induced by t-BHP. On this basis, HUVECs were transfected with adenovirus carrying adiponectin prior to exposure to t-BHP, to further explore the protective effect of adiponectin on apoptosis induced by t-BHP. The percentage of cell viability was determined by MTT assay. The apoptotic rate was evaluated by fluorescence microsopic analysis with Hochest/PI staining. The protein levels of p-JNK, JNK and Caspase 3 were detected by Western blot. Results: Following t-BHP 100 μ mol/L administration for 8 h, the ratio of apoptotic cells was increased. Western blot revealed that the protein levels of p-JNK and active caspase 3 were increased( P <0.01) compared to the control group. When cells were pretreated by adenovirus with adiponectin, the apoptosis rate and protein levels of p-JNK and active caspase 3 were decreased significantly( P <0.01). Conclusions: Continuous exposure to t-BHP induced apoptosis in HUVECs. Recombinant adiponectin protected HUVECs from apoptosis induced by t-BHP, which was correlated with the downregulation of p-JNK and active Caspase 3. |
| فهرسة مساهمة: | Keywords: apoptosis; human umbilical vein endothelial cells; oxidative stress; recombinant adiponectin; tert-butylhydroperoxide |
| المشرفين على المادة: | 0 (Adiponectin) 0 (Recombinant Proteins) 955VYL842B (tert-Butylhydroperoxide) EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases) EC 3.4.22.- (CASP3 protein, human) EC 3.4.22.- (Caspase 3) |
| تواريخ الأحداث: | Date Created: 20180622 Date Completed: 20181018 Latest Revision: 20181018 |
| رمز التحديث: | 20231215 |
| DOI: | 10.13459/j.cnki.cjap.2016.06.012 |
| PMID: | 29926623 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1000-6834 |
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| DOI: | 10.13459/j.cnki.cjap.2016.06.012 |