دورية أكاديمية
Analysis of mitochondrial respiratory function in tissue biopsies and blood cells.
| العنوان: | Analysis of mitochondrial respiratory function in tissue biopsies and blood cells. |
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| المؤلفون: | Ost M; Department of Physiology of Energy Metabolism, German Institute of Human Nutrition Potsdam-Rehbrücke, Nuthetal, Germany., Doerrier C; Oroboros Instruments, Innsbruck, Austria., Gama-Perez P; Department of Physiological Sciences, University of Barcelona, Barcelona, Spain., Moreno-Gomez S; Department of Physiological Sciences, University of Barcelona, Barcelona, Spain. |
| المصدر: | Current opinion in clinical nutrition and metabolic care [Curr Opin Clin Nutr Metab Care] 2018 Sep; Vol. 21 (5), pp. 336-342. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Review |
| اللغة: | English |
| بيانات الدورية: | Publisher: Lippincott Williams & Wilkins Country of Publication: England NLM ID: 9804399 Publication Model: Print Cited Medium: Internet ISSN: 1473-6519 (Electronic) Linking ISSN: 13631950 NLM ISO Abbreviation: Curr Opin Clin Nutr Metab Care Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 1999- : London : Lippincott Williams & Wilkins Original Publication: London ; Philadelphia : Rapid Science Publishers, c1998- |
| مواضيع طبية MeSH: | Oxygen Consumption*, Adipose Tissue/*metabolism , Blood Cells/*metabolism , Liver/*metabolism , Mitochondria/*physiology , Muscle, Skeletal/*metabolism, Adipose Tissue/ultrastructure ; Biopsy ; Blood Cells/ultrastructure ; Humans ; Liver/ultrastructure ; Muscle, Skeletal/ultrastructure ; Oxidative Phosphorylation |
| مستخلص: | Purpose of Review: The review provides an overview on latest methodological strategies to assess mitochondrial respiratory function in tissue biopsies or blood cells. In addition, it summarizes the recent literature related to this topic. Recent Findings: Today, the study of mitochondrial function in key metabolic active tissues has been become more relevant, with increasing focus in clinical applications. In addition, assessment of mitochondrial function in blood cells by respirometry might be a sensitive biomarker of disease progression. High-Resolution Respirometry provides a modern tool to study mitochondrial respiratory physiology which allows direct measurement of cellular metabolic function during health and disease. Moreover, standard operating procedures are required regarding instrumental settings, sample collection and preparation, protocol design and respirometric data analysis of mitochondrial respiratory function in tissue biopsies (such as skeletal muscle, liver and adipose tissue), as well as isolated blood cells. Summary: Mitochondrial function is a key factor in many metabolic diseases. Although various analytical approaches are available, certain well-established protocols for isolated mitochondria are limited for the analysis of mitochondrial function in tissue biopsies or blood cells. Thus, cautious considerations in selecting appropriate protocols and analytical endpoints are crucial for the interpretation of the gained data and to draw robust conclusions. |
| تواريخ الأحداث: | Date Created: 20180626 Date Completed: 20190606 Latest Revision: 20190606 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1097/MCO.0000000000000486 |
| PMID: | 29939971 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1473-6519 |
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| DOI: | 10.1097/MCO.0000000000000486 |