دورية أكاديمية
Fluorescence time-resolved macroimaging.
| العنوان: | Fluorescence time-resolved macroimaging. |
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| المؤلفون: | Shcheslavskiy VI, Shirmanova MV, Dudenkova VV, Lukyanov KA, Gavrina AI, Shumilova AV, Zagaynova E, Becker W |
| المصدر: | Optics letters [Opt Lett] 2018 Jul 01; Vol. 43 (13), pp. 3152-3155. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Optica Publishing Group Country of Publication: United States NLM ID: 7708433 Publication Model: Print Cited Medium: Internet ISSN: 1539-4794 (Electronic) Linking ISSN: 01469592 NLM ISO Abbreviation: Opt Lett Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Publication: Washington, DC : Optica Publishing Group Original Publication: New York, Optical Society of America. |
| مستخلص: | While laser scanning fluorescence lifetime imaging (FLIM) is a powerful approach for cell biology, its small field of view (typically less than 1 mm) makes it impractical for the imaging of large biological samples that is often required for biomedical applications. Here we present a system that allows performing FLIM on macroscopic samples as large as 18 mm with a lateral resolution of 15 μm. The performance of the system is verified with FLIM of endogenous metabolic cofactor reduced nicotinamide adenine dinucleotide (phosphate), NAD(P)H, and genetically encoded fluorescent protein mKate2 in a mouse tumor in vivo. |
| تواريخ الأحداث: | Date Created: 20180630 Date Completed: 20180702 Latest Revision: 20181023 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1364/OL.43.003152 |
| PMID: | 29957804 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1539-4794 |
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| DOI: | 10.1364/OL.43.003152 |