دورية أكاديمية
أعرض في EDS

Phenotype and Viability of MLO-Y4 Cells Is Maintained by TGFβ₃ in a Serum-Dependent Manner within a 3D-Co-Culture with MG-63 Cells.

التفاصيل البيبلوغرافية
العنوان: Phenotype and Viability of MLO-Y4 Cells Is Maintained by TGFβ₃ in a Serum-Dependent Manner within a 3D-Co-Culture with MG-63 Cells.
المؤلفون: Jähn K; Department of Osteology and Biomechanics, University Medical Center Hamburg-Eppendorf, 22529 Hamburg, Germany. k.jaehn@uke.de., Mason DJ; School of Biosciences, Cardiff University, CF10 3AX Cardiff, UK. masondj@cardiff.ac.uk., Ralphs JR; School of Biosciences, Cardiff University, CF10 3AX Cardiff, UK. ralphs@cardiff.ac.uk., Evans BAJ; School of Medicine, Cardiff University, CF14 4XN Cardiff, UK. evansba@cardiff.ac.uk., Archer CW; College of Medicine, Swansea University, SA2 8PP Swansea, UK. c.archer@swansea.ac.uk., Richards RG; School of Biosciences, Cardiff University, CF10 3AX Cardiff, UK. geoff.richards@aofoundation.org.; AO Research Institute Davos, AO Foundation, 7270 Davos, Switzerland. geoff.richards@aofoundation.org., Stoddart MJ; AO Research Institute Davos, AO Foundation, 7270 Davos, Switzerland. martin.stoddart@aofoundation.org.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2018 Jun 30; Vol. 19 (7). Date of Electronic Publication: 2018 Jun 30.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Coculture Techniques/*methods , Osteoblasts/*drug effects , Osteoblasts/*metabolism , Transforming Growth Factor beta/*pharmacology, Animals ; Cell Death/drug effects ; Cell Differentiation/drug effects ; Cell Survival/drug effects ; Immunohistochemistry ; In Situ Nick-End Labeling ; Mice ; Osteocytes/drug effects ; Osteocytes/metabolism
مستخلص: The osteocyte network inside the bone matrix is of functional importance and osteocyte cell death is a characteristic feature of pathological bone diseases. Osteocytes have emerged as key regulators of bone tissue maintenance, yet maintaining their phenotype during in vitro culture remains challenging. A 3D co-culture system for osteocytes with osteoblasts was recently presented, enabling the determination of more physiological effects of growth factors on cells in vitro. MLO-Y4 cells were embedded within a type I collagen gel and cultured in the presence of surface MG-63 cells. Co-culture was performed in the presence or absence of TGFβ₃. Gene expression by quantitative PCR, protein expression by fluorescent immunohistochemistry and cell viability tests were performed. The 3D co-culture induced cell differentiation of MG-63 cells seen by increased type I collagen and osteocalcin mRNA expression. TGFβ₃ maintained osteocyte differentiation of MLO-Y4 cells during co-culture as determined by stable E11 and osteocalcin mRNA expression till day 4. Interestingly, most of the effects of TGFβ₃ on co-cultured cells were serum-dependent. Also, TGFβ₃ reduced cell death of 3D co-cultured MLO-Y4 cells in a serum-dependent manner. This study shows that 3D co-culture upregulates differentiation of MG-63 cells to a more mature osteoblast-like phenotype; while the addition of TGFβ₃ maintained the characteristic MLO-Y4 osteocyte-like phenotype and viability in a serum-dependent manner.
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فهرسة مساهمة: Keywords: bone biology; osteoblast; osteocyte; three-dimensional (3D) co-culture; transforming growth factor-beta 3 (TGFβ3)
المشرفين على المادة: 0 (Transforming Growth Factor beta)
تواريخ الأحداث: Date Created: 20180704 Date Completed: 20181016 Latest Revision: 20190425
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC6073466
DOI: 10.3390/ijms19071932
PMID: 29966376
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms19071932