دورية أكاديمية
أعرض في EDS

Differentially expressed genes in hemocytes of Litopenaeus vannamei challenged with Vibrio parahaemolyticus AHPND (VP AHPND ) and VP AHPND toxin.

التفاصيل البيبلوغرافية
العنوان: Differentially expressed genes in hemocytes of Litopenaeus vannamei challenged with Vibrio parahaemolyticus AHPND (VP AHPND ) and VP AHPND toxin.
المؤلفون: Maralit BA; Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Thailand., Jaree P; Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Thailand., Boonchuen P; Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Thailand., Tassanakajon A; Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Thailand; Omics Science and Bioinformatics Center, Faculty of Science, Chulalongkorn University, Thailand., Somboonwiwat K; Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Thailand; Omics Science and Bioinformatics Center, Faculty of Science, Chulalongkorn University, Thailand. Electronic address: kunlaya.s@chula.ac.th.
المصدر: Fish & shellfish immunology [Fish Shellfish Immunol] 2018 Oct; Vol. 81, pp. 284-296. Date of Electronic Publication: 2018 Jul 18.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: England NLM ID: 9505220 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9947 (Electronic) Linking ISSN: 10504648 NLM ISO Abbreviation: Fish Shellfish Immunol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London ; New York : Academic Press, c1991-
مواضيع طبية MeSH: Bacterial Toxins/*toxicity , Hemocytes/*drug effects , Penaeidae/*genetics , Penaeidae/*immunology , Transcriptome/*drug effects, Animals ; Arthropod Proteins/genetics ; Arthropod Proteins/immunology ; Hemocytes/immunology ; Penaeidae/microbiology ; Vibrio parahaemolyticus
مستخلص: While toxin-harboring Vibrio parahaemolyticus has been previously established as the causative agent of early mortality syndrome (EMS) or acute hepatopancreatic necrosis disease (AHPND) in shrimp, information on the mechanistic processes that happen in the host during infection is still lacking. Here, we examined the expression responses of the shrimp hemocyte transcriptome to V. parahaemolyticus AHPND (VP AHPND ) by RNA sequencing (RNA-seq). Using libraries (SRA accession number SRP137285) prepared from shrimp hemocytes under experimental conditions, a reference library was de novo assembled for gene expression analysis of VP AHPND -challenged samples at 0, 3/6, and 48 h post infection (hpi). Using the library from 0-hpi as the control, 359 transcripts were found to be differentially expressed in the 3/6-hpi library, while 429 were differentially expressed in the 48-hpi library. The expression patterns reported in the RNA-seq of 9 representative genes such as anti-lipopolysaccharide factor (LvALF), crustin p (CRU), serpin 3 (SER), C-type lectin 3 (CTL), clottable protein 2 (CLO), mitogen-activated protein kinase kinase 4 (MKK4), P38 mitogen-activated protein kinase (P38), protein kinase A regulatory subunit 1 (PKA) and DNAJ homolog subfamily C member 1-like (DNJ) were validated by qRT-PCR. The expression of these genes was also analyzed in shrimp that were injected with the partially purified VP AHPND toxin. A VP AHPND toxin-responsive gene, LvALF was identified, and its function was characterized by RNA interference. LvALF knockdown resulted in significantly rapid increase of shrimp mortality caused by toxin injection. Protein-protein interaction analysis by molecular docking suggested that LvALF possibly neutralizes VP AHPND toxin through its LPS-binding domain. The data generated in this study provide preliminary insights into the differences in the immune response of shrimp to the bacterial and toxic aspect of VP AHPND as a disease.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Early mortality syndrome; Hemocyte; Immune system; Shrimp; Transcriptome
المشرفين على المادة: 0 (Arthropod Proteins)
0 (Bacterial Toxins)
تواريخ الأحداث: Date Created: 20180704 Date Completed: 20181211 Latest Revision: 20181211
رمز التحديث: 20231215
DOI: 10.1016/j.fsi.2018.06.054
PMID: 29966688
قاعدة البيانات: MEDLINE
الوصف
تدمد:1095-9947
DOI:10.1016/j.fsi.2018.06.054