دورية أكاديمية
Extracellular vesicles in DLBCL provide abundant clues to aberrant transcriptional programming and genomic alterations.
العنوان: | Extracellular vesicles in DLBCL provide abundant clues to aberrant transcriptional programming and genomic alterations. |
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المؤلفون: | Rutherford SC; Division of Hematology/Oncology, Department of Medicine, Weill Cornell Medicine/New York Presbyterian Hospital, New York, NY., Fachel AA; Division of Hematology/Oncology, Department of Medicine, Weill Cornell Medicine/New York Presbyterian Hospital, New York, NY., Li S; The Jackson Laboratory for Genomic Medicine and The Jackson Laboratory Cancer Center, University of Connecticut Health, Farmington, CT., Sawh S; Division of Hematology/Oncology, Department of Medicine, Weill Cornell Medicine/New York Presbyterian Hospital, New York, NY., Muley A; Division of Hematology/Oncology, Department of Medicine, Weill Cornell Medicine/New York Presbyterian Hospital, New York, NY., Ishii J; Epigenomics Core, Weill Cornell Medicine, New York, NY., Saxena A; Division of Hematology/Oncology, Department of Medicine, Weill Cornell Medicine/New York Presbyterian Hospital, New York, NY., Dominguez PM; Division of Hematology/Oncology, Department of Medicine, Weill Cornell Medicine/New York Presbyterian Hospital, New York, NY., Caldas Lopes E; Division of Hematology/Oncology, Department of Medicine, Weill Cornell Medicine/New York Presbyterian Hospital, New York, NY., Agirre X; Division of Hemato-Oncology, Center for Applied Medical Research CIMA, University of Navarra, Ciberonc, Pamplona, Spain., Chambwe N; Institute for Systems Biology, Seattle, WA., Correa F; Memorial Sloan Kettering Cancer Center, New York, NY., Jiang Y; Division of Hematology/Oncology, Department of Medicine, Weill Cornell Medicine/New York Presbyterian Hospital, New York, NY., Richards KL; Division of Hematology/Oncology, Department of Medicine, Weill Cornell Medicine/New York Presbyterian Hospital, New York, NY., Betel D; Division of Hematology/Oncology, Department of Medicine, Weill Cornell Medicine/New York Presbyterian Hospital, New York, NY.; Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY; and., Shaknovich R; Division of Hematology/Oncology, Department of Medicine, Weill Cornell Medicine/New York Presbyterian Hospital, New York, NY.; Cancer Genetics Inc, Rutherford, NJ. |
المصدر: | Blood [Blood] 2018 Aug 16; Vol. 132 (7), pp. e13-e23. Date of Electronic Publication: 2018 Jul 02. |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 7603509 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1528-0020 (Electronic) Linking ISSN: 00064971 NLM ISO Abbreviation: Blood Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 2021- : [New York] : Elsevier Original Publication: New York, Grune & Stratton [etc.] |
مواضيع طبية MeSH: | Extracellular Vesicles/*metabolism , Lymphoma, Large B-Cell, Diffuse/*metabolism , Neoplasm Proteins/*metabolism , RNA, Neoplasm/*metabolism, Cell Line, Tumor ; Extracellular Vesicles/genetics ; Extracellular Vesicles/pathology ; Humans ; Lymphoma, Large B-Cell, Diffuse/genetics ; Lymphoma, Large B-Cell, Diffuse/pathology ; Neoplasm Proteins/genetics ; RNA, Neoplasm/genetics |
مستخلص: | The biological role of extracellular vesicles (EVs) in diffuse large B-cell lymphoma (DLBCL) initiation and progression remains largely unknown. We characterized EVs secreted by 5 DLBCL cell lines, a primary DLBCL tumor, and a normal control B-cell sample, optimized their purification, and analyzed their content. We found that DLBCLs secreted large quantities of CD63, Alix, TSG101, and CD81 EVs, which can be extracted using an ultracentrifugation-based method and traced by their cell of origin surface markers. We also showed that tumor-derived EVs can be exchanged between lymphoma cells, normal tonsillar cells, and HK stromal cells. We then examined the content of EVs, focusing on isolation of high-quality total RNA. We sequenced the total RNA and analyzed the nature of RNA species, including coding and noncoding RNAs. We compared whole-cell and EV-derived RNA composition in benign and malignant B cells and discovered that transcripts from EVs were involved in many critical cellular functions. Finally, we performed mutational analysis and found that mutations detected in EVs exquisitely represented mutations in the cell of origin. These results enhance our understanding and enable future studies of the role that EVs may play in the pathogenesis of DLBCL, particularly with regards to the exchange of genomic information. Current findings open a new strategy for liquid biopsy approaches in disease monitoring. (© 2018 by The American Society of Hematology.) |
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معلومات مُعتمدة: | P30 CA008748 United States CA NCI NIH HHS; P30 CA034196 United States CA NCI NIH HHS |
المشرفين على المادة: | 0 (Neoplasm Proteins) 0 (RNA, Neoplasm) |
تواريخ الأحداث: | Date Created: 20180704 Date Completed: 20190715 Latest Revision: 20210202 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC6265635 |
DOI: | 10.1182/blood-2017-12-821843 |
PMID: | 29967128 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1528-0020 |
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DOI: | 10.1182/blood-2017-12-821843 |