دورية أكاديمية
Extracellular vesicles in DLBCL provide abundant clues to aberrant transcriptional programming and genomic alterations.
| العنوان: | Extracellular vesicles in DLBCL provide abundant clues to aberrant transcriptional programming and genomic alterations. |
|---|---|
| المؤلفون: | Rutherford SC; Division of Hematology/Oncology, Department of Medicine, Weill Cornell Medicine/New York Presbyterian Hospital, New York, NY., Fachel AA; Division of Hematology/Oncology, Department of Medicine, Weill Cornell Medicine/New York Presbyterian Hospital, New York, NY., Li S; The Jackson Laboratory for Genomic Medicine and The Jackson Laboratory Cancer Center, University of Connecticut Health, Farmington, CT., Sawh S; Division of Hematology/Oncology, Department of Medicine, Weill Cornell Medicine/New York Presbyterian Hospital, New York, NY., Muley A; Division of Hematology/Oncology, Department of Medicine, Weill Cornell Medicine/New York Presbyterian Hospital, New York, NY., Ishii J; Epigenomics Core, Weill Cornell Medicine, New York, NY., Saxena A; Division of Hematology/Oncology, Department of Medicine, Weill Cornell Medicine/New York Presbyterian Hospital, New York, NY., Dominguez PM; Division of Hematology/Oncology, Department of Medicine, Weill Cornell Medicine/New York Presbyterian Hospital, New York, NY., Caldas Lopes E; Division of Hematology/Oncology, Department of Medicine, Weill Cornell Medicine/New York Presbyterian Hospital, New York, NY., Agirre X; Division of Hemato-Oncology, Center for Applied Medical Research CIMA, University of Navarra, Ciberonc, Pamplona, Spain., Chambwe N; Institute for Systems Biology, Seattle, WA., Correa F; Memorial Sloan Kettering Cancer Center, New York, NY., Jiang Y; Division of Hematology/Oncology, Department of Medicine, Weill Cornell Medicine/New York Presbyterian Hospital, New York, NY., Richards KL; Division of Hematology/Oncology, Department of Medicine, Weill Cornell Medicine/New York Presbyterian Hospital, New York, NY., Betel D; Division of Hematology/Oncology, Department of Medicine, Weill Cornell Medicine/New York Presbyterian Hospital, New York, NY.; Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY; and., Shaknovich R; Division of Hematology/Oncology, Department of Medicine, Weill Cornell Medicine/New York Presbyterian Hospital, New York, NY.; Cancer Genetics Inc, Rutherford, NJ. |
| المصدر: | Blood [Blood] 2018 Aug 16; Vol. 132 (7), pp. e13-e23. Date of Electronic Publication: 2018 Jul 02. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 7603509 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1528-0020 (Electronic) Linking ISSN: 00064971 NLM ISO Abbreviation: Blood Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York] : Elsevier Original Publication: New York, Grune & Stratton [etc.] |
| مواضيع طبية MeSH: | Extracellular Vesicles/*metabolism , Lymphoma, Large B-Cell, Diffuse/*metabolism , Neoplasm Proteins/*metabolism , RNA, Neoplasm/*metabolism, Cell Line, Tumor ; Extracellular Vesicles/genetics ; Extracellular Vesicles/pathology ; Humans ; Lymphoma, Large B-Cell, Diffuse/genetics ; Lymphoma, Large B-Cell, Diffuse/pathology ; Neoplasm Proteins/genetics ; RNA, Neoplasm/genetics |
| مستخلص: | The biological role of extracellular vesicles (EVs) in diffuse large B-cell lymphoma (DLBCL) initiation and progression remains largely unknown. We characterized EVs secreted by 5 DLBCL cell lines, a primary DLBCL tumor, and a normal control B-cell sample, optimized their purification, and analyzed their content. We found that DLBCLs secreted large quantities of CD63, Alix, TSG101, and CD81 EVs, which can be extracted using an ultracentrifugation-based method and traced by their cell of origin surface markers. We also showed that tumor-derived EVs can be exchanged between lymphoma cells, normal tonsillar cells, and HK stromal cells. We then examined the content of EVs, focusing on isolation of high-quality total RNA. We sequenced the total RNA and analyzed the nature of RNA species, including coding and noncoding RNAs. We compared whole-cell and EV-derived RNA composition in benign and malignant B cells and discovered that transcripts from EVs were involved in many critical cellular functions. Finally, we performed mutational analysis and found that mutations detected in EVs exquisitely represented mutations in the cell of origin. These results enhance our understanding and enable future studies of the role that EVs may play in the pathogenesis of DLBCL, particularly with regards to the exchange of genomic information. Current findings open a new strategy for liquid biopsy approaches in disease monitoring. (© 2018 by The American Society of Hematology.) |
| References: | J Clin Oncol. 2010 Sep 20;28(27):4184-90. (PMID: 20660832) Blood. 2015 May 21;125(21):3297-305. (PMID: 25833959) Blood. 2016 Dec 8;128(23):2655-2665. (PMID: 27742710) Blood. 2005 Mar 1;105(5):1851-61. (PMID: 15550490) Clin Chim Acta. 2016 Feb 15;454:28-32. (PMID: 26724367) BMC Bioinformatics. 2011 Aug 04;12:323. (PMID: 21816040) Blood. 2014 Mar 13;123(11):1699-708. (PMID: 24385541) Nat Cell Biol. 2007 Jun;9(6):654-9. (PMID: 17486113) Clin Ther. 2014 Jun 1;36(6):847-862.e1. (PMID: 24952935) IEEE Trans Vis Comput Graph. 2014 Dec;20(12):1983-92. (PMID: 26356912) Genome Biol. 2014 Aug 15;15(8):432. (PMID: 25123191) Nat Med. 2012 Jun;18(6):883-91. (PMID: 22635005) Cancer Discov. 2017 Jan;7(1):38-53. (PMID: 27733359) Cell Rep. 2014 Sep 25;8(6):1649-1658. (PMID: 25242326) Bioinformatics. 2013 Jan 1;29(1):15-21. (PMID: 23104886) Blood. 2010 Mar 4;115(9):1755-64. (PMID: 20018914) Bone Marrow Transplant. 2016 Jan;51(1):51-7. (PMID: 26367239) Blood. 2014 Apr 3;123(14):2189-98. (PMID: 24563408) N Engl J Med. 2008 Nov 27;359(22):2313-23. (PMID: 19038878) J Biol Chem. 2016 Sep 16;291(38):19774-85. (PMID: 27440046) Nat Cell Biol. 2015 Jun;17(6):816-26. (PMID: 25985394) Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15336-41. (PMID: 21873242) Genome Res. 2012 Sep;22(9):1760-74. (PMID: 22955987) N Engl J Med. 2010 Apr 15;362(15):1417-29. (PMID: 20393178) Proc Natl Acad Sci U S A. 2008 Sep 9;105(36):13520-5. (PMID: 18765795) Genes Chromosomes Cancer. 2002 Mar;33(3):225-34. (PMID: 11807979) Nature. 2000 Feb 3;403(6769):503-11. (PMID: 10676951) Clin Lymphoma Myeloma Leuk. 2010 Jun;10(3):192-6. (PMID: 20511164) Nat Cell Biol. 2008 Dec;10(12):1470-6. (PMID: 19011622) Nat Rev Immunol. 2008 Jan;8(1):22-33. (PMID: 18097447) Carcinogenesis. 2015 Sep;36(9):1008-18. (PMID: 26054723) Blood. 2018 Apr 12;131(15):1720-1729. (PMID: 29358175) N Engl J Med. 2002 Jan 24;346(4):235-42. (PMID: 11807147) Biology (Basel). 2012 Dec 14;1(3):895-905. (PMID: 24832523) Cancer Lett. 2015 Aug 1;364(1):59-69. (PMID: 25933830) Clin Cancer Res. 2016 Jan 15;22(2):395-404. (PMID: 26369630) Genome Biol. 2014;15(12):550. (PMID: 25516281) |
| معلومات مُعتمدة: | P30 CA008748 United States CA NCI NIH HHS; P30 CA034196 United States CA NCI NIH HHS |
| المشرفين على المادة: | 0 (Neoplasm Proteins) 0 (RNA, Neoplasm) |
| تواريخ الأحداث: | Date Created: 20180704 Date Completed: 20190715 Latest Revision: 20210202 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC6265635 |
| DOI: | 10.1182/blood-2017-12-821843 |
| PMID: | 29967128 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1528-0020 |
|---|---|
| DOI: | 10.1182/blood-2017-12-821843 |