دورية أكاديمية
Epidemiological and genetic characterization of larval stages of Fasciola gigantica in snail intermediate hosts in Karnataka State, India.
| العنوان: | Epidemiological and genetic characterization of larval stages of Fasciola gigantica in snail intermediate hosts in Karnataka State, India. |
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| المؤلفون: | Rajanna R; Centre for Advanced Faculty Training, Veterinary, College, Hebbal, Bengaluru, Karnataka, India-560024., Thimmareddy PM; Centre for Advanced Faculty Training, Veterinary, College, Hebbal, Bengaluru, Karnataka, India-560024., Sengupta PP; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka, India-560064., Siju SJ; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka, India-560064., Mamatha GS; Centre for Advanced Faculty Training, Veterinary, College, Hebbal, Bengaluru, Karnataka, India-560024. |
| المصدر: | Acta parasitologica [Acta Parasitol] 2018 Sep 25; Vol. 63 (3), pp. 609-616. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer International Publishing Country of Publication: Switzerland NLM ID: 9301947 Publication Model: Print Cited Medium: Internet ISSN: 1896-1851 (Electronic) Linking ISSN: 12302821 NLM ISO Abbreviation: Acta Parasitol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2019- : Cham : Springer International Publishing Original Publication: Warszawa : Witold Stefanski Institute Of Parasitology |
| مواضيع طبية MeSH: | Fasciola hepatica/*genetics , Fascioliasis/*veterinary, Animals ; Cercaria ; DNA, Helminth/chemistry ; DNA, Helminth/genetics ; DNA, Ribosomal Spacer/chemistry ; DNA, Ribosomal Spacer/genetics ; Fasciola hepatica/isolation & purification ; Fascioliasis/epidemiology ; Fascioliasis/parasitology ; India/epidemiology ; Larva ; Phylogeny ; Polymerase Chain Reaction/veterinary ; Ruminants ; Sensitivity and Specificity ; Snails/parasitology |
| مستخلص: | Fasciolosis in ruminants in India is caused by the liver fluke Fasciola gigantica. Radix (Lymnaea) spp. are known to carry the infective stages of this parasite. Understanding the seasonal prevalence of F. gigantica infection in the intermediate host is of extreme importance in order to elucidate the transmission dynamics of the parasite. So the present study was designed to determine the bioclimatic distribution of larval stages of F. gigantica in Radix spp. snails as well as to explore the genetic diversity of F. gigantica in three geographical regions (Deccan plateau, Western Ghats and coastal region) of Karnataka. The lymnaeid snails were sampled (n = 2077) for a period of one year (June 2015 to May 2016) at 24 sites. The snails were morphologically identified and the infection status was established through cercarial shedding and nested polymerase chain reaction (PCR) based technique targeting second internal transcribed spacers (ITS-2) of nuclear ribosomal DNA. The sensitivity of PCR (8.2%) for detection of F. gigantica infection within snail is significantly higher than cercarial shedding (4.3%) with an overall prevalence of 5.1%. The prevalence of infection was higher in winter than in the rainy and summer seasons (6.2% instead of 4.6% and 4.3% respectively). Deccan plateau (5.8%) showed a higher prevalence of infection compared to Western Ghats (5.2%) and Coastal region (3.6%). The sequencing ITS-2 region permitted the identification of the parasite as F. gigantica which is having high implication in studying the population genetic structure of the parasite in the country. In conclusion, overall results indicated that Radix spp. snails harboured F. gigantica developmental stages throughout the year and nested PCR was found to be sensitive and specific for detection of F. gigantica infection in snails compared to routine parasitological techniques. |
| فهرسة مساهمة: | Keywords: Fasciola gigantica; ITS-2; genetic characterization; snail |
| المشرفين على المادة: | 0 (DNA, Helminth) 0 (DNA, Ribosomal Spacer) |
| تواريخ الأحداث: | Date Created: 20180706 Date Completed: 20181015 Latest Revision: 20190722 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1515/ap-2018-0070 |
| PMID: | 29975642 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1896-1851 |
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| DOI: | 10.1515/ap-2018-0070 |