دورية أكاديمية
Interfollicular Epidermal Stem Cells: Boosting and Rescuing from Adult Skin.
| العنوان: | Interfollicular Epidermal Stem Cells: Boosting and Rescuing from Adult Skin. |
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| المؤلفون: | Cerqueira MT; 3B's Research Group-Biomaterials, Biodegradable and Biomimetics, Avepark-Parque de Ciência e Tecnologia, Zona Industrial da Gandra, Guimarães, Portugal.; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal., Frias AM; 3B's Research Group-Biomaterials, Biodegradable and Biomimetics, Avepark-Parque de Ciência e Tecnologia, Zona Industrial da Gandra, Guimarães, Portugal.; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal., Reis RL; 3B's Research Group-Biomaterials, Biodegradable and Biomimetics, Avepark-Parque de Ciência e Tecnologia, Zona Industrial da Gandra, Guimarães, Portugal.; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal.; The Discoveries Centre for Regenerative and Precision Medicine, Headquarters at University of Minho, Guimarães, Portugal., Marques AP; 3B's Research Group-Biomaterials, Biodegradable and Biomimetics, Avepark-Parque de Ciência e Tecnologia, Zona Industrial da Gandra, Guimarães, Portugal. apmarques@dep.uminho.pt.; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal. apmarques@dep.uminho.pt.; The Discoveries Centre for Regenerative and Precision Medicine, Headquarters at University of Minho, Guimarães, Portugal. apmarques@dep.uminho.pt. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 1879, pp. 101-110. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Epidermal Cells/*cytology , Skin/*cytology , Stem Cells/*cytology, Adult ; Amides/pharmacology ; Antigens, CD/metabolism ; Biomarkers/metabolism ; Cells, Cultured ; Collagen Type IV/metabolism ; Epidermal Cells/drug effects ; Epidermal Cells/metabolism ; Humans ; Integrin alpha6/metabolism ; Integrin beta1/metabolism ; Keratinocytes/cytology ; Keratinocytes/drug effects ; Keratinocytes/metabolism ; Multipotent Stem Cells/cytology ; Multipotent Stem Cells/drug effects ; Multipotent Stem Cells/metabolism ; Pyridines/pharmacology ; Receptors, Transferrin/metabolism ; Skin/drug effects ; Skin/metabolism ; Stem Cells/drug effects ; Stem Cells/metabolism |
| مستخلص: | Epidermal stem cells (EpSCs) isolation struggle remains, mainly due to the yet essential requirement of well-defined approaches and markers. The herein proposed methodology integrates an assemblage of strategies to accomplish the enrichment of the interfollicular EpSCs multipotent fraction and their subsequent separation from the remaining primary human keratinocytes (hKC) culture. Those include rapid adherence of freshly isolated hKC to collagen type IV through the β1-integrin ligand and Rho-associated protein kinase inhibitor (Rocki) Y-27632 administration to the cultures, followed by an immunomagnetic separation to obtain populations based in the combined CD49f bri /CD71 dim expression. Flow cytometry is the supporting method to analyze the effect of the treatments over the expression rate of early epidermal markers keratins19/5/14 and in correlation to CD49f bri /CD71 dim sub-populations. The step-by-step methodology herein described indulges the boosting and consecutive purification and separation of interfollicular epidermal stem cells, from human keratinocytes cultures. |
| فهرسة مساهمة: | Keywords: Collagen IV; Epidermal stem cells; Flow cytometry; Immunomagnetic separation; Rock inhibitor |
| المشرفين على المادة: | 0 (Amides) 0 (Antigens, CD) 0 (Biomarkers) 0 (CD71 antigen) 0 (Collagen Type IV) 0 (Integrin alpha6) 0 (Integrin beta1) 0 (Pyridines) 0 (Receptors, Transferrin) 138381-45-0 (Y 27632) |
| تواريخ الأحداث: | Date Created: 20180708 Date Completed: 20190603 Latest Revision: 20190603 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1007/7651_2018_146 |
| PMID: | 29980942 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/7651_2018_146 |