دورية أكاديمية
Diosgenin induces genotoxic and mutagenic effects on HepG2 cells.
| العنوان: | Diosgenin induces genotoxic and mutagenic effects on HepG2 cells. |
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| المؤلفون: | Cruz MS; Universidade Federal Do Rio Grande Do Norte, Programa de Pós-Graduação Em Bioquímica, Av. Sen. Salgado Filho 3000, 59078-970, Natal, RN, Brazil., Navoni JA; Universidade Federal Do Rio Grande Do Norte, Programa de Pós-Graduação Em Desenvolvimento e Meio Ambiente, Av. Sen. Salgado Filho 3000, 59078-970, Natal, RN, Brazil., da Costa Xavier LA; Universidade Federal Do Rio Grande Do Norte, Programa de Pós-Graduação Em Bioquímica, Av. Sen. Salgado Filho 3000, 59078-970, Natal, RN, Brazil., Madalena Rocha Silva Teles M; Universidade Federal da Paraíba, Laboratório de Tecnologia Farmacêutica, Cidade Universitária, 58051900, João Pessoa, PB, Brazil., Barbosa-Filho JM; Universidade Federal da Paraíba, Laboratório de Tecnologia Farmacêutica, Cidade Universitária, 58051900, João Pessoa, PB, Brazil., Almeida-Lima J; Universidade Federal Do Rio Grande Do Norte, Programa de Pós-Graduação Em Bioquímica, Av. Sen. Salgado Filho 3000, 59078-970, Natal, RN, Brazil., de Oliveira Rocha HA; Universidade Federal Do Rio Grande Do Norte, Programa de Pós-Graduação Em Bioquímica, Av. Sen. Salgado Filho 3000, 59078-970, Natal, RN, Brazil., do Amaral VS; Universidade Federal Do Rio Grande Do Norte, Programa de Pós-Graduação Em Bioquímica, Av. Sen. Salgado Filho 3000, 59078-970, Natal, RN, Brazil; Universidade Federal Do Rio Grande Do Norte, Programa de Pós-Graduação Em Desenvolvimento e Meio Ambiente, Av. Sen. Salgado Filho 3000, 59078-970, Natal, RN, Brazil. Electronic address: vi.mariga@gmail.com. |
| المصدر: | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2018 Oct; Vol. 120, pp. 98-103. Date of Electronic Publication: 2018 Jul 05. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 8207483 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6351 (Electronic) Linking ISSN: 02786915 NLM ISO Abbreviation: Food Chem Toxicol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Exeter : Elsevier Science Ltd Original Publication: Oxford ; New York : Pergamon Press, c1982- |
| مواضيع طبية MeSH: | Diosgenin/*toxicity , Mutagens/*toxicity, Cell Survival/drug effects ; Comet Assay ; Dioscorea/chemistry ; Hep G2 Cells ; Humans ; Micronucleus Tests ; Tetrazolium Salts/chemistry ; Thiazoles/chemistry |
| مستخلص: | Yam roots and other plants from Dioscorea genus have cultural, nutritional and economic importance to tropical and subtropical regions and have a great amount of diosgenin in its composition. In the present study the cytotoxic, genotoxic and mutagenic potential of diosgenin on HepG2 cells was investigated. Cytotoxicity was assessed using MTT and clonogenic assay. Genotoxic and mutagenic effects were performed using single cell gel electrophoresis and cytokinesis-block micronucleus assay, respectively. A reduction on cell viability was observed due to diosgenin treatment at concentrations higher than 30 μM. A genotoxic effect was shown by comet assay and CBMN. Besides, an increase in micronucleus frequency along with a significant cytostatic effect were observed. Diosgenin elicited DNA damage on HepG2 cells which could not be efficiently repaired contributing to the mutagenic effect observed. Those results suggest that diosgenin deleterious effect could take place through genetic instability, fact that affects the normal cell cycle, leading to cell's death. (Copyright © 2018. Published by Elsevier Ltd.) |
| فهرسة مساهمة: | Keywords: Cytotoxicity; Diosgenin; Genotoxicity; Mutagenicity |
| المشرفين على المادة: | 0 (Mutagens) 0 (Tetrazolium Salts) 0 (Thiazoles) EUY85H477I (thiazolyl blue) K49P2K8WLX (Diosgenin) |
| تواريخ الأحداث: | Date Created: 20180709 Date Completed: 20181120 Latest Revision: 20181120 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.fct.2018.07.011 |
| PMID: | 29981786 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-6351 |
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| DOI: | 10.1016/j.fct.2018.07.011 |