دورية أكاديمية
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Temporal transcriptome profiling of developing seeds reveals a concerted gene regulation in relation to oil accumulation in Pongamia (Millettia pinnata).

التفاصيل البيبلوغرافية
العنوان: Temporal transcriptome profiling of developing seeds reveals a concerted gene regulation in relation to oil accumulation in Pongamia (Millettia pinnata).
المؤلفون: Huang J; Guangdong Key Laboratory of Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, China., Hao X; Guangdong Key Laboratory of Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, China., Jin Y; Guangdong Key Laboratory of Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, China., Guo X; Guangdong Key Laboratory of Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, China., Shao Q; Guangdong Key Laboratory of Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, China., Kumar KS; Department of Biological Sciences, Michigan Technological University, Houghton, MI, 49931, USA., Ahlawat YK; Department of Biological Sciences, Michigan Technological University, Houghton, MI, 49931, USA., Harry DE; TerViva, Oakland, CA, 94612, USA., Joshi CP; Department of Biological Sciences, Michigan Technological University, Houghton, MI, 49931, USA. cpjoshi@mtu.edu., Zheng Y; Guangdong Key Laboratory of Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, China. yzzheng@szu.edu.cn.
المصدر: BMC plant biology [BMC Plant Biol] 2018 Jul 09; Vol. 18 (1), pp. 140. Date of Electronic Publication: 2018 Jul 09.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 100967807 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2229 (Electronic) Linking ISSN: 14712229 NLM ISO Abbreviation: BMC Plant Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2001-
مواضيع طبية MeSH: Gene Expression Regulation, Plant/*genetics , Millettia/*metabolism , Plant Oils/*metabolism , Seeds/*metabolism, Fatty Acids/analysis ; Fatty Acids/metabolism ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Genes, Plant/genetics ; Metabolic Networks and Pathways/genetics ; Millettia/genetics ; Plant Oils/analysis ; Seeds/chemistry ; Seeds/growth & development ; Transcriptome
مستخلص: Background: Pongamia (Millettia pinnata syn. Pongamia pinnata), an oilseed legume species, is emerging as potential feedstock for sustainable biodiesel production. Breeding Pongamia for favorable traits in commercial application will rely on a comprehensive understanding of molecular mechanism regulating oil accumulation during its seed development. To date, only limited genomic or transcript sequences are available for Pongamia, while a temporal transcriptome profiling of developing seeds is still lacking in this species.
Results: In this work, we conducted a time-series analysis of morphological and physiological characters, oil contents and compositions, as well as global gene expression profiles in developing Pongamia seeds. Firstly, three major developmental phases were characterized based on the combined evidences from embryonic shape, seed weight, seed moisture content, and seed color. Then, the gene expression levels at these three phases were quantified by RNA-Seq analyses with three biological replicates from each phase. Nearly 94% of unigenes were expressed at all three phases, whereas only less than 2% of unigenes were exclusively expressed at one of these phases. A total of 8881 differentially expressed genes (DEGs) were identified between phases. Furthermore, the qRT-PCR analyses for 10 DEGs involved in lipid metabolism demonstrated a good reliability of our RNA-Seq data in temporal gene expression profiling. We observed a dramatic increase in seed oil content from the embryogenesis phase to the early seed-filling phase, followed by a steady and moderate increase towards the maximum at the desiccation phase. We proposed that a highly active expression of most genes related to fatty acid (FA) and triacylglycerol (TAG) biosynthesis at the embryogenesis phase might trigger both the substantial oil accumulation and the membrane lipid synthesis for rapid cell proliferation at this phase, while a concerted reactivation of TAG synthesis-related genes at the desiccation phase might further promote storage lipid synthesis to achieve the maximum content of seed oils.
Conclusions: This study not only built a bridge between gene expression profiles and oil accumulation in developing seeds, but also laid a foundation for future attempts on genetic engineering of Pongamia varieties to acquire higher oil yield or improved oil properties for biofuel applications.
References: Physiol Mol Biol Plants. 2016 Apr;22(2):193-205. (PMID: 27436911)
BMC Genomics. 2015 Oct 24;16:858. (PMID: 26499887)
Gene. 2014 Jun 1;542(2):113-21. (PMID: 24680703)
Lipids. 2010 Feb;45(2):145-57. (PMID: 20101470)
Bioinformatics. 2009 Aug 1;25(15):1966-7. (PMID: 19497933)
Plant Physiol. 2013 Jul;162(3):1337-58. (PMID: 23735505)
Annu Rev Plant Biol. 2013;64:189-217. (PMID: 23451786)
PLoS One. 2013 Sep 10;8(9):e73767. (PMID: 24040062)
Plant J. 2006 Mar;45(6):930-41. (PMID: 16507084)
Bioinformatics. 2010 Jan 1;26(1):139-40. (PMID: 19910308)
J Agric Food Chem. 2015 Dec 23;63(50):10811-21. (PMID: 26628196)
Plant Physiol Biochem. 2012 Mar;52:147-53. (PMID: 22245912)
Proc Natl Acad Sci U S A. 2010 May 4;107(18):8063-70. (PMID: 20385809)
PLoS One. 2013;8(3):e59270. (PMID: 23555009)
Front Plant Sci. 2014 Dec 16;5:698. (PMID: 25566273)
Nat Methods. 2008 Jul;5(7):621-8. (PMID: 18516045)
Mol Cell Proteomics. 2007 Dec;6(12):2165-79. (PMID: 17848586)
Bioresour Technol. 2005 Sep;96(13):1425-9. (PMID: 15939268)
Plant J. 2013 Apr;74(2):351-62. (PMID: 23452239)
Plant J. 2011 Dec;68(6):1014-27. (PMID: 21851431)
BMC Plant Biol. 2010 Aug 05;10:160. (PMID: 20687943)
Mol Phylogenet Evol. 2005 Jan;34(1):159-66. (PMID: 15579389)
Annu Rev Plant Physiol Plant Mol Biol. 2001 Jun;52:335-361. (PMID: 11337402)
J Plant Physiol. 2011 Nov 1;168(16):1877-84. (PMID: 21715045)
Bioinformatics. 2005 Sep 15;21(18):3674-6. (PMID: 16081474)
Trends Plant Sci. 2010 Apr;15(4):211-8. (PMID: 20138563)
Annu Rev Plant Biol. 2005;56:253-79. (PMID: 15862096)
DNA Res. 2012 Apr;19(2):195-207. (PMID: 22351699)
Arch Biochem Biophys. 2002 Jul 1;403(1):25-34. (PMID: 12061798)
Plant J. 2008 Aug;55(3):504-13. (PMID: 18410479)
Annu Rev Plant Physiol Plant Mol Biol. 1998 Jun;49:611-641. (PMID: 15012248)
Proc Natl Acad Sci U S A. 2006 Jul 25;103(30):11206-10. (PMID: 16837571)
Sci Rep. 2016 Sep 28;6:34315. (PMID: 27677333)
PLoS One. 2012;7(5):e36522. (PMID: 22574177)
Protoplasma. 2014 May;251(3):703-9. (PMID: 23990110)
BMC Genomics. 2010 Feb 24;11:136. (PMID: 20181280)
PLoS One. 2012;7(2):e30100. (PMID: 22319559)
Bioresour Technol. 2006 Mar;97(4):671-8. (PMID: 15935657)
Biotechnol Biofuels. 2016 Jul 04;9:136. (PMID: 27382413)
Plant Cell Physiol. 2015 Aug;56(8):1624-40. (PMID: 26092973)
J Exp Bot. 2017 Apr 1;68(8):1955-1972. (PMID: 28087653)
معلومات مُعتمدة: 31300275 National Natural Science Foundation of China; 31370289 National Natural Science Foundation of China; 2014ZT05S078 Guangdong Innovation Research Team Fund; JCYJ20140724165855348 Research and Development Foundation of Science and Technology of Shenzhen
فهرسة مساهمة: Keywords: Biofuel; Concerted regulation; Millettia pinnata; Oil accumulation; Seed development; Temporal transcriptome profiling
المشرفين على المادة: 0 (Fatty Acids)
0 (Plant Oils)
تواريخ الأحداث: Date Created: 20180711 Date Completed: 20190212 Latest Revision: 20190215
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC6038193
DOI: 10.1186/s12870-018-1356-8
PMID: 29986660
قاعدة البيانات: MEDLINE
الوصف
تدمد:1471-2229
DOI:10.1186/s12870-018-1356-8