دورية أكاديمية
First identification of Naegleria species and Vahlkampfia ciguana in Nile water, Cairo, Egypt: Seasonal morphology and phylogenetic analysis.
| العنوان: | First identification of Naegleria species and Vahlkampfia ciguana in Nile water, Cairo, Egypt: Seasonal morphology and phylogenetic analysis. |
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| المؤلفون: | El-Badry AA; Department of Microbiology-Medical Parasitology Section, College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia. Electronic address: aelbadry@kasralainy.edu.eg., Aufy SM; Medical Parasitology Department, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt., El-Wakil ES; Parasitology Department, Theodor Bilharz Research Institute, Giza, Egypt., Rizk EM; Medical Parasitology Department, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt., Mahmoud SS; Parasitology Department, Theodor Bilharz Research Institute, Giza, Egypt., Taha NY; Microbiology Department, Theodor Bilharz Research Institute, Giza, Egypt. |
| المصدر: | Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi [J Microbiol Immunol Infect] 2020 Apr; Vol. 53 (2), pp. 259-265. Date of Electronic Publication: 2018 Jul 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: published by Elsevier for the Taiwan Society of Microbiology Country of Publication: England NLM ID: 100956211 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1995-9133 (Electronic) Linking ISSN: 16841182 NLM ISO Abbreviation: J Microbiol Immunol Infect Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Feb. 2010- : Oxford, England : published by Elsevier for the Taiwan Society of Microbiology Original Publication: Taipei, Taiwan : Chinese Society of Microbiology : Chinese Society of Immunology [and] : Infectious Diseases Society of the Republic of China, |
| مواضيع طبية MeSH: | Phylogeny*, Naegleria/*classification , Naegleria/*cytology , Naegleria/*isolation & purification , Water/*parasitology, Base Sequence ; Cross-Sectional Studies ; DNA, Protozoan/genetics ; DNA, Ribosomal ; Egypt ; Eukaryota/classification ; Eukaryota/cytology ; Eukaryota/isolation & purification ; Seasons |
| مستخلص: | Background/purpose: In Egypt, there is a scarcity of data concerning Naegleria (N.) family, with a shortage of phylogenetic studies. This study's aim was molecular detection, sequencing and phylogenetic analysis of morphologically identified Nagleria and to determine natural seasonal distribution of Nagleria species in water sources of Greater Cairo, Egypt. Methods: A total of 120 water samples were collected during each season over a year. Every water sample was filtrated and cultured on non-nutrient agar (NNA). Morphologically positive Nagleria-like isolates were subjected to Nagleria genus and species-specific PCR targeting rDNA gene, PCR products were sequenced and obtained sequences were phylogenetic analyzed. Results: Nile River water was the only source found to contained Naegleria. For the first time in Egypt, Vahlkampfia ciguana and the Naegleria species N.australiensis, N.philippinensis and N.neojejuensis were identified from the Nile water. The pathogenic Naegleria fowleri, previously reported in Egypt, was however not detected in this study. Conclusion: Interestingly, there were no seasonal variations in prevalence of Naegleria spp.; yet, there was seasonal diversity in the water samples of the same site. These newly discovered Vahlkampfiidae in Egyptian aquatic environments indicate the need for further phylogenetic investigations using bigger sample sizes in order to determine their potential risk for human health. (Copyright © 2018. Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: Egypt; Naegleria australiensis; Naegleria neojejuensis; Naegleria philippinensis; Phylogenetic analysis; Vahlkampfia ciguana |
| المشرفين على المادة: | 0 (DNA, Protozoan) 0 (DNA, Ribosomal) 059QF0KO0R (Water) |
| تواريخ الأحداث: | Date Created: 20180715 Date Completed: 20201117 Latest Revision: 20201117 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.jmii.2018.06.003 |
| PMID: | 30006270 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1995-9133 |
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| DOI: | 10.1016/j.jmii.2018.06.003 |