دورية أكاديمية
أعرض في EDS

Cyclooxygenases and lipoxygenases are used by the fungus Podospora anserina to repel nematodes.

التفاصيل البيبلوغرافية
العنوان: Cyclooxygenases and lipoxygenases are used by the fungus Podospora anserina to repel nematodes.
المؤلفون: Ferrari R; Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain (LIED), Univ. Paris Diderot, Paris F-75205, France., Lacaze I; Direction Santé Confort, Division Agents Biologiques et Aérocontaminants, Centre Scientifique et Technique du Bâtiment (CSTB), 84, avenue Jean Jaurès, Marne-la-Vallée Cedex F-77447, France., Le Faouder P; MetaToul-Lipidomic Core Facility, MetaboHUB, Inserm U1048, Toulouse 31 432, France., Bertrand-Michel J; MetaToul-Lipidomic Core Facility, MetaboHUB, Inserm U1048, Toulouse 31 432, France., Oger C; Institut des Biomolécules Max Mousseron, (IBMM), CNRS, ENSCM, Université de Montpellier, UMR 5247, 15 Av. Ch. Flahault, Montpellier Cedex F-34093, France., Galano JM; Institut des Biomolécules Max Mousseron, (IBMM), CNRS, ENSCM, Université de Montpellier, UMR 5247, 15 Av. Ch. Flahault, Montpellier Cedex F-34093, France., Durand T; Institut des Biomolécules Max Mousseron, (IBMM), CNRS, ENSCM, Université de Montpellier, UMR 5247, 15 Av. Ch. Flahault, Montpellier Cedex F-34093, France., Moularat S; Direction Santé Confort, Division Agents Biologiques et Aérocontaminants, Centre Scientifique et Technique du Bâtiment (CSTB), 84, avenue Jean Jaurès, Marne-la-Vallée Cedex F-77447, France., Chan Ho Tong L; Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain (LIED), Univ. Paris Diderot, Paris F-75205, France., Boucher C; Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain (LIED), Univ. Paris Diderot, Paris F-75205, France., Kilani J; Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain (LIED), Univ. Paris Diderot, Paris F-75205, France., Petit Y; Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain (LIED), Univ. Paris Diderot, Paris F-75205, France., Vanparis O; Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain (LIED), Univ. Paris Diderot, Paris F-75205, France., Trannoy C; Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain (LIED), Univ. Paris Diderot, Paris F-75205, France., Brun S; Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain (LIED), Univ. Paris Diderot, Paris F-75205, France., Lalucque H; Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain (LIED), Univ. Paris Diderot, Paris F-75205, France., Malagnac F; Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain (LIED), Univ. Paris Diderot, Paris F-75205, France; Institute for Integrative Biology of the Cell, CEA, CNRS, Université Paris-Sud, Orsay 91400, France., Silar P; Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain (LIED), Univ. Paris Diderot, Paris F-75205, France. Electronic address: philippe.silar@univ-paris-diderot.fr.
المصدر: Biochimica et biophysica acta. General subjects [Biochim Biophys Acta Gen Subj] 2018 Oct; Vol. 1862 (10), pp. 2174-2182. Date of Electronic Publication: 2018 Jul 17.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101731726 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0304-4165 (Print) Linking ISSN: 03044165 NLM ISO Abbreviation: Biochim Biophys Acta Gen Subj Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam : Elsevier
مواضيع طبية MeSH: Fungal Proteins/*metabolism , Insect Repellents/*toxicity , Lipoxygenases/*metabolism , Nematoda/*immunology , Podospora/*enzymology , Prostaglandin-Endoperoxide Synthases/*metabolism , Volatile Organic Compounds/*toxicity, Animals ; Fungal Proteins/genetics ; Gene Expression Regulation, Fungal ; Lipid Peroxidation ; Lipoxygenases/genetics ; Nematoda/drug effects ; Oxylipins/toxicity ; Prostaglandin-Endoperoxide Synthases/genetics ; Volatile Organic Compounds/analysis
مستخلص: Oxylipins are secondary messengers used universally in the living world for communication and defense. The paradigm is that they are produced enzymatically for the eicosanoids and non-enzymatically for the isoprostanoids. They are supposed to be degraded into volatile organic compounds (VOCs) and to participate in aroma production. Some such chemicals composed of eight carbons are also envisoned as alternatives to fossil fuels. In fungi, oxylipins have been mostly studied in Aspergilli and shown to be involved in signalling asexual versus sexual development, mycotoxin production and interaction with the host for pathogenic species. Through targeted gene deletions of genes encoding oxylipin-producing enzymes and chemical analysis of oxylipins and volatile organic compounds, we show that in the distantly-related ascomycete Podospora anserina, isoprostanoids are likely produced enzymatically. We show the disappearance in the mutants lacking lipoxygenases and cyclooxygenases of the production of 10-hydroxy-octadecadienoic acid and that of 1-octen-3-ol, a common volatile compound. Importantly, this was correlated with the inability of the mutants to repel nematodes as efficiently as the wild type. Overall, our data show that in this fungus, oxylipins are not involved in signalling development but may rather be used directly or as precursors in the production of odors against potential agressors.
Significance: We analyzse the role in inter-kingdom communication of lipoxygenase (lox) and cyclooxygenase (cox) genes in the model fungus Podospora anserina. Through chemical analysis we define the oxylipins and volatile organic compounds (VOCs)produce by wild type and mutants for cox and lox genes, We show that the COX and LOX genes are required for the production of some eight carbon VOCs. We show that COX and LOX genes are involved in the production of chemicals repelling nematodes. This role is very different from the ones previously evidenced in other fungi.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Cyclooxygenase; Fungi; Lipoxygenase; Oxylipins; Podospora anserina; Volatile organic compounds
المشرفين على المادة: 0 (Fungal Proteins)
0 (Insect Repellents)
0 (Oxylipins)
0 (Volatile Organic Compounds)
EC 1.13.11.- (Lipoxygenases)
EC 1.14.99.1 (Prostaglandin-Endoperoxide Synthases)
تواريخ الأحداث: Date Created: 20180721 Date Completed: 20190523 Latest Revision: 20190523
رمز التحديث: 20221213
DOI: 10.1016/j.bbagen.2018.07.012
PMID: 30025856
قاعدة البيانات: MEDLINE
الوصف
تدمد:0304-4165
DOI:10.1016/j.bbagen.2018.07.012