دورية أكاديمية
أعرض في EDS

Genomic screening of new putative antiviral lectins from Amazonian cyanobacteria based on a bioinformatics approach.

التفاصيل البيبلوغرافية
العنوان: Genomic screening of new putative antiviral lectins from Amazonian cyanobacteria based on a bioinformatics approach.
المؤلفون: Siqueira AS; Laboratório de Tecnologia Biomolecular - Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém-, Pennsylvania, Brazil., Lima ARJ; Laboratório de Tecnologia Biomolecular - Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém-, Pennsylvania, Brazil., Aguiar DCF; Laboratório de Tecnologia Biomolecular - Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém-, Pennsylvania, Brazil., Santos AS; Laboratórios de Investigação Sistemática em Biotecnologia e Biodiversidade Molecular - Instituto de Ciências Naturais - Universidade Federal do Pará, Belém-Pennsylvania, Brazil., Vianez Júnior JLDSG; Centro de Inovações Tecnológicas - Instituto Evandro Chagas, Ananindeua-Pennsylvania, Brazil., Gonçalves EC; Laboratório de Tecnologia Biomolecular - Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém-, Pennsylvania, Brazil.
المصدر: Proteins [Proteins] 2018 Oct; Vol. 86 (10), pp. 1047-1054. Date of Electronic Publication: 2018 Sep 25.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Wiley-Liss Country of Publication: United States NLM ID: 8700181 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-0134 (Electronic) Linking ISSN: 08873585 NLM ISO Abbreviation: Proteins Subsets: MEDLINE
أسماء مطبوعة: Publication: New York, NY : Wiley-Liss
Original Publication: New York : Alan R. Liss, c1986-
مواضيع طبية MeSH: Antiviral Agents/*chemistry , Bacterial Proteins/*chemistry , Carrier Proteins/*chemistry , Cyanobacteria/*chemistry , Lectins/*chemistry, Genomics ; Models, Molecular ; Molecular Dynamics Simulation ; Nostoc/chemistry ; Thermodynamics
مستخلص: Lectins are proteins of nonimmune origin, which are capable of recognizing and binding to glycoconjugate moieties. Some of them can block the interaction of viral glycoproteins to the host cell receptors acting as antiviral agents. Although cyanobacterial lectins have presented broad biotechnological potential, little research has been directed to Amazonian Cyanobacterial diversity. In order to identify new antiviral lectins, we performed genomic analysis in seven cyanobacterial strains from Coleção Amazônica de Cianobactérias e Microalgas (CACIAM). We found 75 unique CDS presenting one or more lectin domains. Since almost all were annotated as hypothetical proteins, we used homology modeling and molecular dynamics simulations to evaluate the structural and functional properties of three CDS that were more similar to known antiviral lectins. Nostoc sp. CACIAM 19 as well as Tolypothrix sp. CACIAM 22 strains presented cyanovirin-N homologues whose function was confirmed by binding free energy calculations. Asn, Glu, Thr, Lys, Leu, and Gly, which were described as binding residues for cyanovirin, were also observed on those structures. As for other known cyanovirins, those residues in both our models also made favorable interactions with dimannose. Finally, Alkalinema sp. CACIAM 70d presented one CDS, which was identified as a seven-bladed beta-propeller structure with binding sites predicted for sialic acid and N-acetylglucosamine. Despite its singular structure, our analysis suggested this molecule as a new putative antiviral lectin. Overall, the identification and the characterization of new lectins and their homologues are a promising area in antiviral research, and Amazonian cyanobacteria present biotechnological potential to be explored in this regard.
(© 2018 Wiley Periodicals, Inc.)
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معلومات مُعتمدة: ICAAF 099/2014 International Fundação Amazônia Paraense de Amparo à Pesquisa; 311686/2015-0 International Conselho Nacional de Desenvolvimento Científico e Tecnológico
فهرسة مساهمة: Keywords: Amazonia; antiviral lectins; cyanobacteria; cyanovirin; molecular dynamics
المشرفين على المادة: 0 (Antiviral Agents)
0 (Bacterial Proteins)
0 (Carrier Proteins)
0 (Lectins)
184539-38-6 (cyanovirin N)
تواريخ الأحداث: Date Created: 20180724 Date Completed: 20190830 Latest Revision: 20200422
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC7167734
DOI: 10.1002/prot.25577
PMID: 30035823
قاعدة البيانات: MEDLINE
الوصف
تدمد:1097-0134
DOI:10.1002/prot.25577