دورية أكاديمية
Increasing tolerance of hospital Enterococcus faecium to handwash alcohols.
| العنوان: | Increasing tolerance of hospital Enterococcus faecium to handwash alcohols. |
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| المؤلفون: | Pidot SJ; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia., Gao W; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia., Buultjens AH; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia., Monk IR; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia., Guerillot R; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia., Carter GP; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia., Lee JYH; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia., Lam MMC; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia., Grayson ML; Infectious Diseases Department, Austin Health, Heidelberg, Victoria 3084, Australia.; Department of Medicine, University of Melbourne, Heidelberg, Victoria 3084, Australia.; Department of Epidemiology and Preventive Medicine, Monash University, Victoria 3800, Australia., Ballard SA; Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia., Mahony AA; Infectious Diseases Department, Austin Health, Heidelberg, Victoria 3084, Australia., Grabsch EA; Infectious Diseases Department, Austin Health, Heidelberg, Victoria 3084, Australia., Kotsanas D; Monash Infectious Diseases, Monash Health, Clayton, Victoria 3168, Australia., Korman TM; Monash Infectious Diseases, Monash Health, Clayton, Victoria 3168, Australia., Coombs GW; Antimicrobial Resistance and Infectious Diseases Research Laboratory, School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia.; Department of Microbiology, PathWest Laboratory Medicine WA, Fiona Stanley Hospital, Murdoch, Western Australia 6150, Australia., Robinson JO; Antimicrobial Resistance and Infectious Diseases Research Laboratory, School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia.; Department of Microbiology, PathWest Laboratory Medicine WA, Fiona Stanley Hospital, Murdoch, Western Australia 6150, Australia., Gonçalves da Silva A; Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia., Seemann T; Melbourne Bioinformatics, University of Melbourne, Carlton, Victoria 3053, Australia., Howden BP; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia.; Infectious Diseases Department, Austin Health, Heidelberg, Victoria 3084, Australia.; Department of Medicine, University of Melbourne, Heidelberg, Victoria 3084, Australia.; Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia., Johnson PDR; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia. tstinear@unimelb.edu.au paul.johnson@austin.org.au.; Infectious Diseases Department, Austin Health, Heidelberg, Victoria 3084, Australia.; Department of Medicine, University of Melbourne, Heidelberg, Victoria 3084, Australia., Stinear TP; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Victoria 3010, Australia. tstinear@unimelb.edu.au paul.johnson@austin.org.au. |
| المصدر: | Science translational medicine [Sci Transl Med] 2018 Aug 01; Vol. 10 (452). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 101505086 Publication Model: Print Cited Medium: Internet ISSN: 1946-6242 (Electronic) Linking ISSN: 19466234 NLM ISO Abbreviation: Sci Transl Med Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : American Association for the Advancement of Science |
| مواضيع طبية MeSH: | Hand Disinfection*, Adaptation, Physiological/*drug effects , Alcohols/*toxicity , Enterococcus faecium/*drug effects, 2-Propanol/toxicity ; Animals ; Cross Infection/microbiology ; Enterococcus faecium/genetics ; Enterococcus faecium/isolation & purification ; Female ; Humans ; Mice, Inbred BALB C ; Reproducibility of Results ; Time Factors |
| مستخلص: | Alcohol-based disinfectants and particularly hand rubs are a key way to control hospital infections worldwide. Such disinfectants restrict transmission of pathogens, such as multidrug-resistant Staphylococcus aureus and Enterococcus faecium Despite this success, health care infections caused by E. faecium are increasing. We tested alcohol tolerance of 139 hospital isolates of E. faecium obtained between 1997 and 2015 and found that E. faecium isolates after 2010 were 10-fold more tolerant to killing by alcohol than were older isolates. Using a mouse gut colonization model of E. faecium transmission, we showed that alcohol-tolerant E. faecium resisted standard 70% isopropanol surface disinfection, resulting in greater mouse gut colonization compared to alcohol-sensitive E. faecium We next looked for bacterial genomic signatures of adaptation. Alcohol-tolerant E. faecium accumulated mutations in genes involved in carbohydrate uptake and metabolism. Mutagenesis confirmed the roles of these genes in the tolerance of E. faecium to isopropanol. These findings suggest that bacterial adaptation is complicating infection control recommendations, necessitating additional procedures to prevent E. faecium from spreading in hospital settings. (Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.) |
| التعليقات: | Comment in: J Hosp Infect. 2019 Apr;101(4):423-425. (PMID: 30273640) |
| المشرفين على المادة: | 0 (Alcohols) ND2M416302 (2-Propanol) |
| تواريخ الأحداث: | Date Created: 20180803 Date Completed: 20191011 Latest Revision: 20191011 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1126/scitranslmed.aar6115 |
| PMID: | 30068573 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1946-6242 |
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| DOI: | 10.1126/scitranslmed.aar6115 |