دورية أكاديمية
O-Glycosylation with O-linked β-N-acetylglucosamine increases vascular contraction: Possible modulatory role on Interleukin-10 signaling pathway.
| العنوان: | O-Glycosylation with O-linked β-N-acetylglucosamine increases vascular contraction: Possible modulatory role on Interleukin-10 signaling pathway. |
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| المؤلفون: | Miguez JSG; Institute of Biological and Health Sciences, Federal University of Mato Grosso, Barra do Garças, MT, Brazil., Dela Justina V; Institute of Biological and Health Sciences, Federal University of Mato Grosso, Barra do Garças, MT, Brazil., Bressan AFM; Institute of Biological and Health Sciences, Federal University of Mato Grosso, Barra do Garças, MT, Brazil., Marchi PGF; Institute of Biological and Health Sciences, Federal University of Mato Grosso, Barra do Garças, MT, Brazil., Honorio-França AC; Institute of Biological and Health Sciences, Federal University of Mato Grosso, Barra do Garças, MT, Brazil., Carneiro FS; Department of Pharmacology, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, SP, Brazil., Clinton Webb R; Department of Physiology, Augusta University, Augusta, GA, United States., Tostes RC; Department of Pharmacology, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, SP, Brazil., Giachini FR; Institute of Biological and Health Sciences, Federal University of Mato Grosso, Barra do Garças, MT, Brazil., Lima VV; Institute of Biological and Health Sciences, Federal University of Mato Grosso, Barra do Garças, MT, Brazil. Electronic address: vvlimaufmt@gmail.com. |
| المصدر: | Life sciences [Life Sci] 2018 Sep 15; Vol. 209, pp. 78-84. Date of Electronic Publication: 2018 Jul 31. |
| نوع المنشور: | Journal Article; Review |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0375521 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0631 (Electronic) Linking ISSN: 00243205 NLM ISO Abbreviation: Life Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2008->: Amsterdam : Elsevier Original Publication: Oxford; Elmsford, N. Y. [etc.] Pergamon Press. |
| مواضيع طبية MeSH: | Protein Processing, Post-Translational* , Vasoconstriction*, Acetylglucosamine/*chemistry , Interleukin-10/*chemistry , Interleukin-10/*metabolism, Animals ; Glycosylation ; Signal Transduction |
| مستخلص: | Aims: The interleukin-10 (IL-10) is an immuno-regulatory cytokine that plays a protective effect in the vasculature. IL-10 binding to its receptor, activating the IL-10/JAK1/STAT3 cascade to exert its effects. Therefore, STAT3 phosphorylation is essential for IL-10 actions. O-Glycosylation with linked β-N-acetylglucosamine (O-GlcNAc) is a post-translational modification able to regulate many proteins by interfering with protein on a phosphorylation level. Our aim was to determine whether O-GlcNAc promotes the inhibition of IL-10-pathway (JAK1/STAT3/IL-10), inactivationg its action in the vasculature. Main Methods: Mice (C57BL/6) aortic segments were incubated with vehicle or Thiamet G (0.1 mM, for 24 h) to increase global O-GlcNAc levels. Aortas from knockout mice for IL-10 were also used. Vascular reactivity and western blot tests were performed to evaluate protein expression. Key Findings: High levels of O-GlcNAc, induced by Thiamet G incubation, increased vascular expression of JAK1, but decreased expression and activity of STAT3. In addition, IL-10 levels were diminished in arteries treated with Thiamet G. Absence of IL-10, as well as augmented O-GlcNAcylation, increased vascular reactivity to constrictor stimuli, an effect that was abolished by ERK 1/2 inhibitor. High levels of O-GlcNAc and the absence of IL-10 also leads to increased vascular expression of ERK1/2. Significance: Our data suggest that O-GlcNAc modification seems to (dys)regulate IL-10 signaling pathway and consequently, compromise the protective effect of this cytokine in vasculature. It is possible that there is a promising relationship in pathophysiological conditions where changes in O-GlcNAcylation and IL-10 levels are observed, such as hypertension and diabetes. (Copyright © 2018 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Cytokine; ERK 1/2; O-GlcNAc; Vascular dysfunction |
| المشرفين على المادة: | 130068-27-8 (Interleukin-10) V956696549 (Acetylglucosamine) |
| تواريخ الأحداث: | Date Created: 20180804 Date Completed: 20181003 Latest Revision: 20181004 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.lfs.2018.07.058 |
| PMID: | 30075176 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0631 |
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| DOI: | 10.1016/j.lfs.2018.07.058 |