دورية أكاديمية
The tyrosine kinase v-Src causes mitotic slippage by phosphorylating an inhibitory tyrosine residue of Cdk1.
| العنوان: | The tyrosine kinase v-Src causes mitotic slippage by phosphorylating an inhibitory tyrosine residue of Cdk1. |
|---|---|
| المؤلفون: | Horiuchi M; From the Department of Biochemistry and Molecular Biology, Kyoto Pharmaceutical University, Kyoto 607-8414., Kuga T; From the Department of Biochemistry and Molecular Biology, Kyoto Pharmaceutical University, Kyoto 607-8414., Saito Y; From the Department of Biochemistry and Molecular Biology, Kyoto Pharmaceutical University, Kyoto 607-8414., Nagano M; the Laboratory of Proteome Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka 567-0085, and., Adachi J; the Laboratory of Proteome Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka 567-0085, and., Tomonaga T; the Laboratory of Proteome Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka 567-0085, and., Yamaguchi N; the Laboratory of Molecular Cell Biology, Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260-8675, Japan., Nakayama Y; From the Department of Biochemistry and Molecular Biology, Kyoto Pharmaceutical University, Kyoto 607-8414, nakayama@mb.kyoto-phu.ac.jp. |
| المصدر: | The Journal of biological chemistry [J Biol Chem] 2018 Oct 05; Vol. 293 (40), pp. 15524-15537. Date of Electronic Publication: 2018 Aug 22. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1083-351X (Electronic) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Original Publication: Baltimore, MD : American Society for Biochemistry and Molecular Biology |
| مواضيع طبية MeSH: | Antineoplastic Agents, Phytogenic/*pharmacology , CDC2 Protein Kinase/*genetics , Microtubules/*drug effects , Mitosis/*drug effects , Oncogene Protein pp60(v-src)/*genetics , Paclitaxel/*pharmacology, CDC2 Protein Kinase/antagonists & inhibitors ; CDC2 Protein Kinase/metabolism ; Cell Proliferation/drug effects ; HeLa Cells ; Humans ; Microtubules/metabolism ; Microtubules/ultrastructure ; Oncogene Protein pp60(v-src)/antagonists & inhibitors ; Oncogene Protein pp60(v-src)/metabolism ; Phosphoproteins/genetics ; Phosphoproteins/metabolism ; Phosphorylation ; Polyploidy ; Protein Kinase Inhibitors/pharmacology ; Pyrimidines/pharmacology ; RNA, Small Interfering/genetics ; RNA, Small Interfering/metabolism ; Time-Lapse Imaging |
| مستخلص: | The nonreceptor tyrosine kinase v-Src is an oncogene first identified in Rous sarcoma virus. The oncogenic effects of v-Src have been intensively studied; however, its effects on chromosomal integrity are not fully understood. Here, using HeLa S3/v-Src cells having inducible v-Src expression, we found that v-Src causes mitotic slippage in addition to cytokinesis failure, even when the spindle assembly checkpoint is not satisfied because of the presence of microtubule-targeting agents. v-Src's effect on mitotic slippage was also observed in cells after a knockdown of C-terminal Src kinase (Csk), a protein-tyrosine kinase that inhibits Src-family kinases and was partially inhibited by PP2, an Src-family kinase inhibitor. Proteomic analysis and in vitro kinase assay revealed that v-Src phosphorylates cyclin-dependent kinase 1 (Cdk1) at Tyr-15. This phosphorylation attenuated Cdk1 kinase activity, resulting in a decrease in the phosphorylation of Cdk1 substrates. Furthermore, v-Src-induced mitotic slippage reduced the sensitivity of the cells to microtubule-targeting agents, and cells that survived the microtubule-targeting agents exhibited polyploidy. These results suggest that v-Src causes mitotic slippage by attenuating Cdk1 kinase activity via direct phosphorylation of Cdk1 at Tyr-15. On the basis of these findings, we propose a model for v-Src-induced oncogenesis, in which v-Src-promoted mitotic slippage due to Cdk1 phosphorylation generates genetic diversity via abnormal cell division of polyploid cells and also increases the tolerance of cancer cells to microtubule-targeting agents. (© 2018 Horiuchi et al.) |
| References: | Trends Cell Biol. 2001 Dec;11(12):512-9. (PMID: 11719058) Cell. 1988 Mar 25;52(6):801-10. (PMID: 2450676) J Biol Chem. 2005 Oct 28;280(43):36502-9. (PMID: 16118207) J Biol Chem. 2012 Jul 20;287(30):24905-15. (PMID: 22689581) Science. 1995 Sep 15;269(5230):1567-9. (PMID: 7545311) Biochim Biophys Acta. 2002 Jun 21;1602(2):114-30. (PMID: 12020799) Dev Cell. 2005 Dec;9(6):781-90. (PMID: 16326390) Cancer Res. 1991 Apr 15;51(8):2212-22. (PMID: 2009540) J Cell Biol. 2008 Apr 21;181(2):195-202. (PMID: 18411311) Cell. 1991 Oct 4;67(1):189-96. (PMID: 1655274) Mol Cell. 2010 Mar 26;37(6):753-67. (PMID: 20347419) EMBO J. 2001 Nov 1;20(21):6037-49. (PMID: 11689444) Mol Cell Proteomics. 2009 Dec;8(12):2770-7. (PMID: 19767571) Cell. 1989 Jun 2;57(5):775-86. (PMID: 2470513) Nat Rev Cancer. 2004 Apr;4(4):253-65. (PMID: 15057285) J Biol Chem. 2002 Apr 26;277(17):14666-73. (PMID: 11847223) EMBO J. 1991 Feb;10(2):305-16. (PMID: 1846803) Int J Mol Sci. 2016 Jun 02;17(6):. (PMID: 27271602) Nature. 2012 Jan 18;482(7383):53-8. (PMID: 22258507) EMBO J. 1999 May 4;18(9):2459-71. (PMID: 10228160) Sci Rep. 2016 Dec 12;6:38751. (PMID: 27941902) Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10930-5. (PMID: 11535829) Annu Rev Cell Dev Biol. 1997;13:513-609. (PMID: 9442882) J Biol Chem. 1996 Jan 12;271(2):695-701. (PMID: 8557675) J Cell Biochem. 2016 Apr;117(4):894-903. (PMID: 26365631) Exp Cell Res. 2009 Sep 10;315(15):2515-28. (PMID: 19527713) Nature. 2009 Jul 9;460(7252):278-82. (PMID: 19506557) Mol Cell. 2000 Jan;5(1):13-25. (PMID: 10678165) Arch Biochem Biophys. 2007 Oct 1;466(1):116-24. (PMID: 17692281) Nat Biotechnol. 2008 Dec;26(12):1367-72. (PMID: 19029910) Sci Rep. 2018 Jan 18;8(1):1063. (PMID: 29348492) Cell. 1995 Dec 29;83(7):1159-69. (PMID: 8548803) J Proteome Res. 2014 Dec 5;13(12):5461-70. (PMID: 25230287) Curr Biol. 2006 Jun 20;16(12):1194-200. (PMID: 16782009) Mol Cell Biol. 2000 Sep;20(17):6518-36. (PMID: 10938128) Cell Signal. 2017 Jan;30:19-29. (PMID: 27871934) J Cell Sci. 2009 Aug 1;122(Pt 15):2579-85. (PMID: 19625502) Cancer Cell. 2008 Aug 12;14(2):111-22. (PMID: 18656424) J Cell Sci. 2004 Mar 1;117(Pt 7):989-98. (PMID: 14996930) Mol Cell. 2007 Apr 27;26(2):301-10. (PMID: 17466630) Cell. 1991 Oct 4;67(1):197-211. (PMID: 1913817) Science. 1993 Dec 24;262(5142):2050-4. (PMID: 8266103) J Proteome Res. 2012 Nov 2;11(11):5311-22. (PMID: 22985185) Nature. 2004 Aug 19;430(7002):908-13. (PMID: 15282614) J Biol Chem. 2002 Apr 12;277(15):12487-90. (PMID: 11839732) Mol Cell Proteomics. 2010 Feb;9(2):336-50. (PMID: 19786723) Exp Cell Res. 2013 Jun 10;319(10):1382-97. (PMID: 23562843) J Cell Biochem. 2016 Jun;117(6):1340-51. (PMID: 26529125) J Cell Biochem. 2014 Apr;115(4):763-71. (PMID: 24453048) J Proteome Res. 2008 Feb;7(2):731-40. (PMID: 18183947) Mol Cell Biol. 2011 Apr;31(7):1478-91. (PMID: 21262764) J Biol Chem. 1996 Nov 1;271(44):27847-54. (PMID: 8910383) J Biol Chem. 2007 Feb 23;282(8):5327-39. (PMID: 17189253) Int J Mol Sci. 2017 Apr 12;18(4):null. (PMID: 28417908) Cell. 2014 Aug 14;158(4):833-848. (PMID: 25126788) |
| فهرسة مساهمة: | Keywords: Cdk1; Src; cancer; cell cycle; cyclin-dependent kinase (CDK); microtubule-targeting agent; mitosis; mitotic slippage; pTyr-15; phosphorylation; spindle checkpoint; tyrosine kinase; v-Src |
| المشرفين على المادة: | 0 (AG 1879) 0 (Antineoplastic Agents, Phytogenic) 0 (Phosphoproteins) 0 (Protein Kinase Inhibitors) 0 (Pyrimidines) 0 (RNA, Small Interfering) EC 2.7.10.2 (Oncogene Protein pp60(v-src)) EC 2.7.11.22 (CDC2 Protein Kinase) EC 2.7.11.22 (CDK1 protein, human) P88XT4IS4D (Paclitaxel) |
| تواريخ الأحداث: | Date Created: 20180824 Date Completed: 20190304 Latest Revision: 20210205 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC6177586 |
| DOI: | 10.1074/jbc.RA118.002784 |
| PMID: | 30135207 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1083-351X |
|---|---|
| DOI: | 10.1074/jbc.RA118.002784 |