دورية أكاديمية
Candida species biotypes in the oral cavity of infants and children with orofacial clefts under surgical rehabilitation.
| العنوان: | Candida species biotypes in the oral cavity of infants and children with orofacial clefts under surgical rehabilitation. |
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| المؤلفون: | Silva JJD; Laboratory of Microbiology and Immunology, Department of Oral Diagnosis, Dental School of Piracicaba, State University of Campinas (FOP/UNICAMP), Piracicaba, 13414-903, SP, Brazil; Laboratory of Pharmacogenetics and Molecular Biology, Faculty of Medical Sciences, University of Alfenas (UNIFENAS), Alfenas, 37132-440, MG, Brazil. Electronic address: jefersonbiomed@hotmail.com., Silva TAD; Laboratory of Pharmacogenetics and Molecular Biology, Faculty of Medical Sciences, University of Alfenas (UNIFENAS), Alfenas, 37132-440, MG, Brazil., Almeida H; Laboratory of Pharmacogenetics and Molecular Biology, Faculty of Medical Sciences, University of Alfenas (UNIFENAS), Alfenas, 37132-440, MG, Brazil., Rodrigues Netto MF; Laboratory of Microbiology and Immunology, Department of Oral Diagnosis, Dental School of Piracicaba, State University of Campinas (FOP/UNICAMP), Piracicaba, 13414-903, SP, Brazil., Cerdeira CD; Laboratory of Biochemistry, Biomedical Science Institute, Federal University of Alfenas (UNIFAL-MG), Alfenas, 37130-001, MG, Brazil., Höfling JF; Laboratory of Microbiology and Immunology, Department of Oral Diagnosis, Dental School of Piracicaba, State University of Campinas (FOP/UNICAMP), Piracicaba, 13414-903, SP, Brazil., Boriollo MFG; Laboratory of Microbiology and Immunology, Department of Oral Diagnosis, Dental School of Piracicaba, State University of Campinas (FOP/UNICAMP), Piracicaba, 13414-903, SP, Brazil; Laboratory of Pharmacogenetics and Molecular Biology, Faculty of Medical Sciences, University of Alfenas (UNIFENAS), Alfenas, 37132-440, MG, Brazil. |
| المصدر: | Microbial pathogenesis [Microb Pathog] 2018 Nov; Vol. 124, pp. 203-215. Date of Electronic Publication: 2018 Aug 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: England NLM ID: 8606191 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-1208 (Electronic) Linking ISSN: 08824010 NLM ISO Abbreviation: Microb Pathog Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London ; Orlando : Academic Press, c1986- |
| مواضيع طبية MeSH: | Candida/*isolation & purification , Candidiasis/*microbiology , Cleft Lip/*microbiology , Cleft Lip/*surgery , Mouth/*microbiology, Antifungal Agents/pharmacology ; Candida/classification ; Candida/drug effects ; Candida/genetics ; Child ; Child, Preschool ; Drug Resistance, Fungal ; Female ; Humans ; Infant ; Male ; Microbial Sensitivity Tests ; Mycological Typing Techniques |
| مستخلص: | Patients with orofacial clefts present various risk factors for oral infectious diseases, resulting from anatomical and physiological changes and those resulting from rehabilitating therapeutic interventions. The incidence of Candida species in groups of babies and children with orofacial clefts, during pre- and post-operative periods and until return to first consultation, and the profiles for antifungal sensitivity and virulence in vitro were investigated. Oral samples were collected at different times over the surgical procedures and post-surgical clinical consultation and seeded in chromogenic culture media CHROMagar Candida ® . Candida biotypes were identified by accessing species-specific genomic DNA sequences by PCR techniques and electrophoretic procedures. Antifungal susceptibility testing was performed by the method of microdilution in broth using the antifungals amphotericin B (AP), nystatin (NYS) and fluconazole (FLC). SAP and PL exoenzyme activities were determined by classical microbiological methods. Some orofacial clefts occurred preferentially in male or female. Low incidence (39.1%) of oral colonization by Candida species (C. albicans, C. krusei, C. tropicalis and Candida spp.) was reported in patient admission to surgical ward, with no correlation to orofacial cleft types or surgical history. Significant reduction in frequencies of Candida and changes of species, over sampling periods, showed dynamic patterns of oral colonization: elimination, maintenance or neocolonization of the biotypes. These biotypes showed sensitivity to AP (100%), partial resistance to FLC (<10%) and variable MICs for NYS (0.125-4 μg/mL), in addition to strong exoenzyme activities, especially for SAP. Clinical and therapeutic conducts for surgical rehabilitation, anatomical and physiological characteristics of patients with orofacial clefts, and cultural behavior and regionalism of the patient population served could influence the frequencies and dynamics of oral colonization by Candida species. The data showed Candida biotypes resistant to FLC and sensitive (AP) or clinically compatible (NYS) to polyenes, especially C. albicans, in the oral cavity of patients predisposed to oral colonization and candidiases, contributing to clinical conducts in possible antifungal therapies. These biotypes were considered potentially virulent and able to partially modulate their virulence factors, especially SAP, under the conditions favored by host. (Copyright © 2018 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antifungals; Candida species; Cleft lip and palate; PCR; Virulence |
| المشرفين على المادة: | 0 (Antifungal Agents) |
| تواريخ الأحداث: | Date Created: 20180824 Date Completed: 20190306 Latest Revision: 20190306 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.micpath.2018.08.042 |
| PMID: | 30138757 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-1208 |
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| DOI: | 10.1016/j.micpath.2018.08.042 |