دورية أكاديمية
أعرض في EDS

Transcriptional and epigenetic profiling of nutrient-deprived cells to identify novel regulators of autophagy.

التفاصيل البيبلوغرافية
العنوان: Transcriptional and epigenetic profiling of nutrient-deprived cells to identify novel regulators of autophagy.
المؤلفون: Peeters JGC; a Center for Molecular Medicine , University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands.; b Laboratory of Translational Immunology , University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands.; c Division of Pediatrics , Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands.; e Regenerative Medicine Center , University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands., Picavet LW; b Laboratory of Translational Immunology , University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands.; c Division of Pediatrics , Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands.; e Regenerative Medicine Center , University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands., Coenen SGJM; b Laboratory of Translational Immunology , University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands.; c Division of Pediatrics , Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands.; e Regenerative Medicine Center , University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands., Mauthe M; d Department of Cell Biology , University Medical Center Groningen, University of Groningen , Groningen , The Netherlands., Vervoort SJ; a Center for Molecular Medicine , University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands., Mocholi E; a Center for Molecular Medicine , University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands.; e Regenerative Medicine Center , University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands., de Heus C; a Center for Molecular Medicine , University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands.; f Department of Cell Biology , University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands., Klumperman J; a Center for Molecular Medicine , University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands.; f Department of Cell Biology , University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands., Vastert SJ; b Laboratory of Translational Immunology , University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands.; c Division of Pediatrics , Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands., Reggiori F; d Department of Cell Biology , University Medical Center Groningen, University of Groningen , Groningen , The Netherlands., Coffer PJ; a Center for Molecular Medicine , University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands.; c Division of Pediatrics , Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands.; e Regenerative Medicine Center , University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands., Mokry M; c Division of Pediatrics , Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands.; e Regenerative Medicine Center , University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands.; g Epigenomics facility , University Medical Center Utrecht , Utrecht , The Netherlands., van Loosdregt J; a Center for Molecular Medicine , University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands.; b Laboratory of Translational Immunology , University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands.; c Division of Pediatrics , Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands.; e Regenerative Medicine Center , University Medical Center Utrecht, Utrecht University , Utrecht , The Netherlands.
المصدر: Autophagy [Autophagy] 2019 Jan; Vol. 15 (1), pp. 98-112. Date of Electronic Publication: 2018 Sep 11.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101265188 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1554-8635 (Electronic) Linking ISSN: 15548627 NLM ISO Abbreviation: Autophagy Subsets: MEDLINE
أسماء مطبوعة: Publication: 2015- : Philadelphia, PA : Taylor & Francis
Original Publication: Georgetown, TX : Landes Bioscience, 2005-
مواضيع طبية MeSH: Epigenesis, Genetic* , Gene Expression Profiling*, Autophagy/*physiology , Early Growth Response Protein 1/*metabolism , Lysosomes/*metabolism, Early Growth Response Protein 1/genetics ; Gene Expression Regulation ; HEK293 Cells ; Humans ; Nutrients
مستخلص: Macroautophagy (hereafter autophagy) is a lysosomal degradation pathway critical for maintaining cellular homeostasis and viability, and is predominantly regarded as a rapid and dynamic cytoplasmic process. To increase our understanding of the transcriptional and epigenetic events associated with autophagy, we performed extensive genome-wide transcriptomic and epigenomic profiling after nutrient deprivation in human autophagy-proficient and autophagy-deficient cells. We observed that nutrient deprivation leads to the transcriptional induction of numerous autophagy-associated genes. These transcriptional changes are reflected at the epigenetic level (H3K4me3, H3K27ac, and H3K56ac) and are independent of autophagic flux. As a proof of principle that this resource can be used to identify novel autophagy regulators, we followed up on one identified target: EGR1 (early growth response 1), which indeed appears to be a central transcriptional regulator of autophagy by affecting autophagy-associated gene expression and autophagic flux. Taken together, these data stress the relevance of transcriptional and epigenetic regulation of autophagy and can be used as a resource to identify (novel) factors involved in autophagy regulation.
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فهرسة مساهمة: Keywords: Autophagy; ChIP-seq; EGR1; RNA-seq; nutrient-deprivation
المشرفين على المادة: 0 (Early Growth Response Protein 1)
تواريخ الأحداث: Date Created: 20180829 Date Completed: 20200312 Latest Revision: 20200312
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC6287694
DOI: 10.1080/15548627.2018.1509608
PMID: 30153076
قاعدة البيانات: MEDLINE
الوصف
تدمد:1554-8635
DOI:10.1080/15548627.2018.1509608