دورية أكاديمية
أعرض في EDS

Impact of autologous whole blood administration upon experimental mouse models of acute Trypanosoma cruzi infection.

التفاصيل البيبلوغرافية
العنوان: Impact of autologous whole blood administration upon experimental mouse models of acute Trypanosoma cruzi infection.
المؤلفون: Philot Pavão B; 1Laboratório de Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ Brazil., Demarque KC; 1Laboratório de Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ Brazil., Meuser Batista M; 1Laboratório de Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ Brazil., Melo de Oliveira G; 1Laboratório de Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ Brazil., França da Silva C; 1Laboratório de Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ Brazil., Guedes da Silva FH; 1Laboratório de Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ Brazil., Gonçalves Caputo LF; 2Laboratório de Patologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ Brazil., Machado Cascabulho C; 3Laboratório de Inovações em Terapias, Ensino e Bioprodutos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ Brazil., Barcinski MA; 1Laboratório de Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ Brazil., Correia Soeiro MN; 1Laboratório de Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ Brazil.
المصدر: The journal of venomous animals and toxins including tropical diseases [J Venom Anim Toxins Incl Trop Dis] 2018 Aug 30; Vol. 24, pp. 25. Date of Electronic Publication: 2018 Aug 30 (Print Publication: 2018).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: CEVAP Country of Publication: Brazil NLM ID: 101201501 Publication Model: eCollection Cited Medium: Print ISSN: 1678-9199 (Print) Linking ISSN: 16789180 NLM ISO Abbreviation: J Venom Anim Toxins Incl Trop Dis Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: 2019- : Botucatu, SP, Brazil : CEVAP
Original Publication: Botucatu, SP, Brazil : CEVAP, c2003-
مستخلص: Background: Autologous whole blood (AWB) administration is described as alternative/complementary medical practice widely employed in medical and veterinary therapy against infections, chronic pathologies and neoplasias. Our aim is to investigate in vivo biological effect of AWB using healthy murine models under the course of Trypanosoma cruzi acute infection.
Methods: The first set of studies consisted of injecting different volumes of AWB and saline (SAL) into the posterior region of quadriceps muscle of healthy male Swiss mice under distinct therapeutic schemes evaluating: animal behavior, body and organ weight, hemogram, plasmatic biochemical markers for tissue damage and inflammatory cytokine levels and profile. To assess the impact on the experimental T. cruzi infection, different schemes (prior and post infection) and periods of AWB administration (from one up to 10 days) were conducted, also employing heterologous whole blood (HWB) and evaluating plasma cytokine profile.
Results: No major adverse events were observed in healthy AWB-treated mice, except gait impairment in animals that received three doses of 20 μL AWB in the same hind limb. AWB and SAL triggered an immediate polymorphonuclear response followed by mononuclear infiltrate. Although SAL triggered an inflammatory response, the kinetics and intensity of the histological profile and humoral mediator levels were different from AWB, the latter occurring earlier and more intensely with concomitant elevation of plasma IL-6. Inflammatory peak response of SAL, mainly composed of mononuclear cells with IL-10, was increased at 24 h. According to the mouse model of acute T. cruzi infection, only minor decreases (< 30%) in the parasitemia levels were produced by AWB and HWB given before and after infection, without protecting against mortality. Rises in IFN-gamma, TNF-alpha and IL-6 were detected at 9 dpi in all infected animals as compared to uninfected mice but only Bz displayed a statistically significant diminution ( p =  0.02) in TNF-alpha levels than infected and untreated mice.
Conclusions: This study revealed that the use of autologous whole blood (AWB) in the acute model employed was unable to reduce the parasitic load of infected mice, providing only a minor decrease in parasitemia levels (up to 30%) but without protecting against animal mortality. Further in vivo studies will be necessary to elucidate the effective impact of this procedure.
Competing Interests: Our present study was submitted to Fiocruz Committee of Ethics for the Use of Animals (CEUA) and approved under the license number CEUA L-032/2016.Not applicable.The authors declare that they have no competing interests.Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
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فهرسة مساهمة: Keywords: Alternative therapy; Autologous blood; Mouse models; Trypanosoma cruzi
تواريخ الأحداث: Date Created: 20180907 Latest Revision: 20220321
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC6117903
DOI: 10.1186/s40409-018-0157-8
PMID: 30186314
قاعدة البيانات: MEDLINE
الوصف
تدمد:1678-9199
DOI:10.1186/s40409-018-0157-8