دورية أكاديمية
PRODUCTION OF SINGLE CHAIN FRAGMENT VARIABLE (scFv) ANTIBODY AGAINST PARANOSEMA LOCUSTAE ALPHA/BETA-HYDROLASE AND IMMUNOLOCALIZATION OF MICROSPORIDIAN ENZYME IN THE INFECTED HOST CELL.
| العنوان: | PRODUCTION OF SINGLE CHAIN FRAGMENT VARIABLE (scFv) ANTIBODY AGAINST PARANOSEMA LOCUSTAE ALPHA/BETA-HYDROLASE AND IMMUNOLOCALIZATION OF MICROSPORIDIAN ENZYME IN THE INFECTED HOST CELL. |
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| المؤلفون: | Dolgikh VV, Tsarev AA, Senderskiy IV, Timofeev SA |
| المصدر: | Tsitologiia [Tsitologiia] 2017; Vol. 59 (4), pp. 298-305. |
| نوع المنشور: | Journal Article |
| اللغة: | English; Russian |
| بيانات الدورية: | Publisher: Rossiiskaia Akademiia Nauk Country of Publication: Russia (Federation) NLM ID: 0417363 Publication Model: Print Cited Medium: Print ISSN: 0041-3771 (Print) Linking ISSN: 00413771 NLM ISO Abbreviation: Tsitologiia Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Moskva : Rossiiskaia Akademiia Nauk Original Publication: Moskva, Akademiia nauk SSSR. |
| مواضيع طبية MeSH: | Antibodies, Fungal*/chemistry , Antibodies, Fungal*/genetics , Antibodies, Fungal*/immunology , Single-Chain Antibodies*/chemistry , Single-Chain Antibodies*/genetics , Single-Chain Antibodies*/immunology, Fat Body/*microbiology , Fungal Proteins/*immunology , Grasshoppers/*microbiology , Hydrolases/*immunology , Microsporidia/*immunology, Animals ; Mice |
| مستخلص: | Microsporidia is a widespread group of fungi-related intracellular parasites. Direct contact of the most microsporidia species with host cytoplasm suggests that these parasites may control physiological processes of infected cells by secretion of various proteins. In previous experiments, secretion of significant amounts of microsporidia Paranosema locustae alpha/beta-hydrolase into infected cells of Locusta migratoria fat bodies was demonstrated using polyclonal antibodies against the enzyme. However, heterologous expression of microsporidian hydrolase in yeast Pichia pastoris cells was not accompanied by its secretion. In this study, we have constructed library of recombinant single chain antibodies (scFv-fragments) against proteins of fat bodies of infected locusts and isolated mini-antibody specifically recognizing the studied enzyme using phage display technology. Immunoblotting and immunofluorescent microscopy with selected scFv-fragment confirmed secretion of two different in size forms of P. locustae alpha/beta-hydrolase into infected host cell. Prospects of scFv-fragment use to explore the role of microsporidian hydrolase in host-parasite relations and mechanism of its secretion are discussed in the paper. |
| المشرفين على المادة: | 0 (Antibodies, Fungal) 0 (Fungal Proteins) 0 (Single-Chain Antibodies) EC 3.- (Hydrolases) |
| تواريخ الأحداث: | Date Created: 20180907 Date Completed: 20180924 Latest Revision: 20180924 |
| رمز التحديث: | 20231215 |
| PMID: | 30188096 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0041-3771 |
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