دورية أكاديمية
AGGREGATION BY LECTIN-METHODICAL APPROACH FOR EFFECTIVE ISOLATION OF EXOSOMES FROM CELL CULTURE SUPERNATANT FOR PROTEOME PROFILING.
| العنوان: | AGGREGATION BY LECTIN-METHODICAL APPROACH FOR EFFECTIVE ISOLATION OF EXOSOMES FROM CELL CULTURE SUPERNATANT FOR PROTEOME PROFILING. |
|---|---|
| المؤلفون: | Shtam TA, Burdakov VS, Landa SB, Naryzhny SN, Bairamukov VY, Malek AV, Orlov YN, Filatov MV |
| المصدر: | Tsitologiia [Tsitologiia] 2017; Vol. 59 (1), pp. 5-12. |
| نوع المنشور: | Journal Article |
| اللغة: | English; Russian |
| بيانات الدورية: | Publisher: Rossiiskaia Akademiia Nauk Country of Publication: Russia (Federation) NLM ID: 0417363 Publication Model: Print Cited Medium: Print ISSN: 0041-3771 (Print) Linking ISSN: 00413771 NLM ISO Abbreviation: Tsitologiia Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Moskva : Rossiiskaia Akademiia Nauk Original Publication: Moskva, Akademiia nauk SSSR. |
| مواضيع طبية MeSH: | Exosomes*/chemistry , Exosomes*/metabolism, Lectins/*chemistry , Proteome/*metabolism , Proteomics/*methods, HeLa Cells ; Humans ; MCF-7 Cells |
| مستخلص: | Exosomes are small membrane vesicles secreted by most cell types in vivo and in vitro. Exosomes are found in cell culture media, blood, urine, amniotic fluid, malignant ascite fluids and contain distinct subsets of microRNAs and proteins depending upon the tissue from which they are secreted. Thus exosomes constitute potential biomarkers of human diseases, such as cancer. A major bottleneck in the development of exosome-based diagnostic assays is the challenging purification of these vesicles; this requires time-consuming and instrument-based procedures. Isolation of exosomes can be a tedious, non-specific, and difficult process. Here, we provide a preparative technique for isolation of exosomes based on their ability to aggregate in the presence of lectins. The new method for lectin-based isolation of exosomes from cell culture media was developed as a sample preparation step for exosome-based protein biomarker research. |
| المشرفين على المادة: | 0 (Lectins) 0 (Proteome) |
| تواريخ الأحداث: | Date Created: 20180907 Date Completed: 20180924 Latest Revision: 20180924 |
| رمز التحديث: | 20231215 |
| PMID: | 30188097 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0041-3771 |
|---|