دورية أكاديمية
أعرض في EDS

Cancer immunophenotyping by seven-colour multispectral imaging without tyramide signal amplification.

التفاصيل البيبلوغرافية
العنوان: Cancer immunophenotyping by seven-colour multispectral imaging without tyramide signal amplification.
المؤلفون: Ijsselsteijn ME; Department of Pathology, Leiden University Medical Centre, Leiden, The Netherlands., Brouwer TP; Department of Pathology, Leiden University Medical Centre, Leiden, The Netherlands.; Department of Surgery, Leiden University Medical Centre, Leiden, The Netherlands., Abdulrahman Z; Department of Pathology, Leiden University Medical Centre, Leiden, The Netherlands., Reidy E; Department of Pathology, Leiden University Medical Centre, Leiden, The Netherlands., Ramalheiro A; Department of Pathology, Leiden University Medical Centre, Leiden, The Netherlands., Heeren AM; Department of Gynaecology, Leiden University Medical Centre, Centre for Gynaecologic Oncology, Amsterdam, The Netherlands., Vahrmeijer A; Department of Surgery, Leiden University Medical Centre, Leiden, The Netherlands., Jordanova ES; Department of Pathology, Leiden University Medical Centre, Leiden, The Netherlands.; Department of Gynaecology, Leiden University Medical Centre, Centre for Gynaecologic Oncology, Amsterdam, The Netherlands., de Miranda NF; Department of Pathology, Leiden University Medical Centre, Leiden, The Netherlands.
المصدر: The journal of pathology. Clinical research [J Pathol Clin Res] 2019 Jan; Vol. 5 (1), pp. 3-11. Date of Electronic Publication: 2018 Dec 04.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Wiley Blackwell Country of Publication: England NLM ID: 101658534 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2056-4538 (Electronic) Linking ISSN: 20564538 NLM ISO Abbreviation: J Pathol Clin Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford : Wiley Blackwell, [2015]-
مواضيع طبية MeSH: Immunophenotyping*, Biomarkers, Tumor/*analysis , Lymphocytes, Tumor-Infiltrating/*pathology , Neoplasms/*pathology, Biomarkers, Tumor/genetics ; Fluorescent Antibody Technique/methods ; Humans ; Immunotherapy/methods ; Lymphocytes, Tumor-Infiltrating/metabolism ; Neoplasms/diagnosis ; Neoplasms/genetics ; Sequence Analysis, RNA/methods
مستخلص: Checkpoint blockade immunotherapies have revolutionised cancer treatment in the last decade. Nevertheless, these are only beneficial for a small proportion of cancer patients. Important prognosticators for response to immunotherapy are the mutation burden of tumours as well as the quality and quantity of tumour-infiltrating immune cells. High-throughput multiplex immunophenotyping technologies have a central role in deciphering the complexity of anti-tumour immune responses. Current techniques for the immunophenotyping of solid tumours are held back by the lack of spatial context, limitations in the number of targets that can be visualised simultaneously, and/or cumbersome protocols. We developed a tyramide signal amplification-free method for the simultaneous detection of seven cellular targets by immunofluorescence. This method overcomes limitations posed by most widespread techniques and provides a unique tool for extensive phenotyping by multispectral fluorescence microscopy. Furthermore, it can be easily implemented as a high-throughput technology for validation of discovery sets generated by RNA sequencing or mass cytometry and may serve in the future as a complementary diagnostic tool.
(© 2018 The Authors. The Journal of Pathology: Clinical Research published by The Pathological Society of Great Britain and Ireland and John Wiley & Sons Ltd.)
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فهرسة مساهمة: Keywords: T-cells; biomarkers; cancer microenvironment; immune infiltration; immunotherapy; multispectral immunofluorescence; myeloid cells
المشرفين على المادة: 0 (Biomarkers, Tumor)
تواريخ الأحداث: Date Created: 20180908 Date Completed: 20200722 Latest Revision: 20200722
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC6317065
DOI: 10.1002/cjp2.113
PMID: 30191683
قاعدة البيانات: MEDLINE
الوصف
تدمد:2056-4538
DOI:10.1002/cjp2.113