دورية أكاديمية
Cancer immunophenotyping by seven-colour multispectral imaging without tyramide signal amplification.
العنوان: | Cancer immunophenotyping by seven-colour multispectral imaging without tyramide signal amplification. |
---|---|
المؤلفون: | Ijsselsteijn ME; Department of Pathology, Leiden University Medical Centre, Leiden, The Netherlands., Brouwer TP; Department of Pathology, Leiden University Medical Centre, Leiden, The Netherlands.; Department of Surgery, Leiden University Medical Centre, Leiden, The Netherlands., Abdulrahman Z; Department of Pathology, Leiden University Medical Centre, Leiden, The Netherlands., Reidy E; Department of Pathology, Leiden University Medical Centre, Leiden, The Netherlands., Ramalheiro A; Department of Pathology, Leiden University Medical Centre, Leiden, The Netherlands., Heeren AM; Department of Gynaecology, Leiden University Medical Centre, Centre for Gynaecologic Oncology, Amsterdam, The Netherlands., Vahrmeijer A; Department of Surgery, Leiden University Medical Centre, Leiden, The Netherlands., Jordanova ES; Department of Pathology, Leiden University Medical Centre, Leiden, The Netherlands.; Department of Gynaecology, Leiden University Medical Centre, Centre for Gynaecologic Oncology, Amsterdam, The Netherlands., de Miranda NF; Department of Pathology, Leiden University Medical Centre, Leiden, The Netherlands. |
المصدر: | The journal of pathology. Clinical research [J Pathol Clin Res] 2019 Jan; Vol. 5 (1), pp. 3-11. Date of Electronic Publication: 2018 Dec 04. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Wiley Blackwell Country of Publication: England NLM ID: 101658534 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2056-4538 (Electronic) Linking ISSN: 20564538 NLM ISO Abbreviation: J Pathol Clin Res Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Oxford : Wiley Blackwell, [2015]- |
مواضيع طبية MeSH: | Immunophenotyping*, Biomarkers, Tumor/*analysis , Lymphocytes, Tumor-Infiltrating/*pathology , Neoplasms/*pathology, Biomarkers, Tumor/genetics ; Fluorescent Antibody Technique/methods ; Humans ; Immunotherapy/methods ; Lymphocytes, Tumor-Infiltrating/metabolism ; Neoplasms/diagnosis ; Neoplasms/genetics ; Sequence Analysis, RNA/methods |
مستخلص: | Checkpoint blockade immunotherapies have revolutionised cancer treatment in the last decade. Nevertheless, these are only beneficial for a small proportion of cancer patients. Important prognosticators for response to immunotherapy are the mutation burden of tumours as well as the quality and quantity of tumour-infiltrating immune cells. High-throughput multiplex immunophenotyping technologies have a central role in deciphering the complexity of anti-tumour immune responses. Current techniques for the immunophenotyping of solid tumours are held back by the lack of spatial context, limitations in the number of targets that can be visualised simultaneously, and/or cumbersome protocols. We developed a tyramide signal amplification-free method for the simultaneous detection of seven cellular targets by immunofluorescence. This method overcomes limitations posed by most widespread techniques and provides a unique tool for extensive phenotyping by multispectral fluorescence microscopy. Furthermore, it can be easily implemented as a high-throughput technology for validation of discovery sets generated by RNA sequencing or mass cytometry and may serve in the future as a complementary diagnostic tool. (© 2018 The Authors. The Journal of Pathology: Clinical Research published by The Pathological Society of Great Britain and Ireland and John Wiley & Sons Ltd.) |
References: | Science. 2006 Sep 29;313(5795):1960-4. (PMID: 17008531) J Histochem Cytochem. 2007 Jun;55(6):545-54. (PMID: 17242468) Clin Cancer Res. 2008 Apr 1;14(7):2028-35. (PMID: 18381941) J Clin Oncol. 2009 Jan 10;27(2):186-92. (PMID: 19064967) J Histochem Cytochem. 2009 Oct;57(10):899-905. (PMID: 19365090) J Surg Res. 2011 May 15;167(2):e211-9. (PMID: 19765725) Cancer Immunol Immunother. 2010 May;59(5):653-61. (PMID: 19908042) N Engl J Med. 2010 Aug 19;363(8):711-23. (PMID: 20525992) Pathology. 2010;42(5):409-13. (PMID: 20632815) Clin Cancer Res. 2012 Mar 1;18(5):1237-45. (PMID: 22261803) Nat Rev Cancer. 2012 Mar 15;12(4):298-306. (PMID: 22419253) Trends Immunol. 2012 Jul;33(7):323-32. (PMID: 22476049) Leukemia. 2012 Sep;26(9):1908-75. (PMID: 22552007) N Engl J Med. 2012 Jun 28;366(26):2443-54. (PMID: 22658127) N Engl J Med. 2012 Jun 28;366(26):2455-65. (PMID: 22658128) Hum Pathol. 2013 Jan;44(1):29-38. (PMID: 22944297) Science. 2015 Apr 3;348(6230):124-8. (PMID: 25765070) Genome Biol. 2015 Mar 31;16:64. (PMID: 25853550) N Engl J Med. 2015 Jun 25;372(26):2509-20. (PMID: 26028255) Sci Rep. 2015 Sep 24;5:14273. (PMID: 26399191) Clin Cancer Res. 2016 Jun 15;22(12):2908-18. (PMID: 26787752) J Immunother Cancer. 2016 Jan 19;4:3. (PMID: 26788324) Cancer Immunol Immunother. 2016 Apr;65(4):393-403. (PMID: 26899388) Clin Cancer Res. 2016 Apr 15;22(8):1845-55. (PMID: 27084738) Clin Cancer Res. 2016 Apr 15;22(8):1865-74. (PMID: 27084740) Nat Med. 2016 Jun;22(6):679-84. (PMID: 27111280) Immunity. 2016 May 17;44(5):1227-39. (PMID: 27178470) Nat Rev Cancer. 2016 Jul;16(7):447-62. (PMID: 27339708) Lancet Oncol. 2016 Dec;17(12):e542-e551. (PMID: 27924752) Cell Rep. 2017 Apr 4;19(1):203-217. (PMID: 28380359) Science. 2017 Jul 28;357(6349):409-413. (PMID: 28596308) Cell. 2017 Sep 7;170(6):1120-1133.e17. (PMID: 28803728) Sci Rep. 2017 Sep 4;7(1):10376. (PMID: 28871164) Cell. 2017 Nov 2;171(4):934-949.e16. (PMID: 29033130) Sci Rep. 2017 Oct 17;7(1):13380. (PMID: 29042640) |
فهرسة مساهمة: | Keywords: T-cells; biomarkers; cancer microenvironment; immune infiltration; immunotherapy; multispectral immunofluorescence; myeloid cells |
المشرفين على المادة: | 0 (Biomarkers, Tumor) |
تواريخ الأحداث: | Date Created: 20180908 Date Completed: 20200722 Latest Revision: 20200722 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC6317065 |
DOI: | 10.1002/cjp2.113 |
PMID: | 30191683 |
قاعدة البيانات: | MEDLINE |
كن أول من يترك تعليقا!