دورية أكاديمية
Ultrasensitive quantitative detection of small molecules with rapid lateral-flow assay based on high-affinity bifunctional ligand and magnetic nanolabels.
| العنوان: | Ultrasensitive quantitative detection of small molecules with rapid lateral-flow assay based on high-affinity bifunctional ligand and magnetic nanolabels. |
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| المؤلفون: | Znoyko SL; Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St, Moscow, 119991, Russia., Orlov AV; Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St, Moscow, 119991, Russia; Moscow Institute of Physics and Technology, 9 Institutskii per, Dolgoprudny, Moscow Region, 141700, Russia., Pushkarev AV; Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St, Moscow, 119991, Russia; Moscow Institute of Physics and Technology, 9 Institutskii per, Dolgoprudny, Moscow Region, 141700, Russia., Mochalova EN; Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St, Moscow, 119991, Russia; Moscow Institute of Physics and Technology, 9 Institutskii per, Dolgoprudny, Moscow Region, 141700, Russia., Guteneva NV; Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St, Moscow, 119991, Russia; Moscow Institute of Physics and Technology, 9 Institutskii per, Dolgoprudny, Moscow Region, 141700, Russia., Lunin AV; Moscow Institute of Physics and Technology, 9 Institutskii per, Dolgoprudny, Moscow Region, 141700, Russia., Nikitin MP; Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St, Moscow, 119991, Russia; Moscow Institute of Physics and Technology, 9 Institutskii per, Dolgoprudny, Moscow Region, 141700, Russia., Nikitin PI; Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St, Moscow, 119991, Russia; National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), 31 Kashirskoe shosse, Moscow, 115409, Russia. Electronic address: nikitin@kapella.gpi.ru. |
| المصدر: | Analytica chimica acta [Anal Chim Acta] 2018 Nov 30; Vol. 1034, pp. 161-167. Date of Electronic Publication: 2018 Jul 06. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0370534 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4324 (Electronic) Linking ISSN: 00032670 NLM ISO Abbreviation: Anal Chim Acta Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Amsterdam. |
| مواضيع طبية MeSH: | Chromatography, Affinity*, Magnetite Nanoparticles/*chemistry , Small Molecule Libraries/*analysis , Thyroxine/*blood, Antibodies, Monoclonal/immunology ; Biotin/chemistry ; Humans ; Ligands ; Small Molecule Libraries/chemistry ; Thyroxine/chemistry ; Thyroxine/immunology |
| مستخلص: | An ultrasensitive lateral-flow assay is developed for rapid quantitative detection of small molecules on-site. The conceptual novelty, which transfers lateral-flow assays to the category of highly sensitive quantitative systems, is due to employment of a bifunctional ligand combined with volumetric registration of magnetic nanolabels. The ligand provides extremely high affinity for trapping the nanolabels and, simultaneously, efficiently competes with the analyzed molecules for the limited quantity of antigen-binding sites on the nanolabels. The developed assay has been demonstrated as the first express method for measuring in human serum of free thyroxine (fT4). The limit of detection is 20 fМ or 16 fg/ml at the assay time <30 min with the dynamic range of 3 orders. Besides, we present the results of first characterization of kinetic parameters of interaction between free thyroxine and monoclonal antibody, as well as of competitive relationship between fT4 and fT4-biotin. The proposed universal platform can be used for ultrasensitive detection of small molecules in human in vitro diagnostics, veterinary, biosafety and counter-terrorism, food quality control, environmental monitoring, etc., as well as for search of new, previously undetectable, diagnostic markers in medicine. (Copyright © 2018 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Biosensing; Low-molecular substances; Magnetic nanoparticles; Real-time monitoring of biomolecular binding; Small molecules detection; Ultrasensitive immunoassay |
| المشرفين على المادة: | 0 (Antibodies, Monoclonal) 0 (Ligands) 0 (Magnetite Nanoparticles) 0 (Small Molecule Libraries) 6SO6U10H04 (Biotin) Q51BO43MG4 (Thyroxine) |
| تواريخ الأحداث: | Date Created: 20180909 Date Completed: 20181218 Latest Revision: 20181218 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.aca.2018.07.012 |
| PMID: | 30193630 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-4324 |
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| DOI: | 10.1016/j.aca.2018.07.012 |