دورية أكاديمية
Mycobacterium tuberculosis cording in alveolar macrophages of patients with pulmonary tuberculosis is likely associated with increased mycobacterial virulence.
| العنوان: | Mycobacterium tuberculosis cording in alveolar macrophages of patients with pulmonary tuberculosis is likely associated with increased mycobacterial virulence. |
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| المؤلفون: | Ufimtseva EG; The Research Institute of Biochemistry, Federal Research Center of Fundamental and Translation Medicine, 2 Timakova Street, 630117, Novosibirsk, Russia; Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health of the Russian Federation, 50 XXII Partsyezda, 620039, Yekaterinburg, Russia. Electronic address: ufim1@ngs.ru., Eremeeva NI; Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health of the Russian Federation, 50 XXII Partsyezda, 620039, Yekaterinburg, Russia. Electronic address: eremeevani@yandex.ru., Petrunina EM; Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health of the Russian Federation, 50 XXII Partsyezda, 620039, Yekaterinburg, Russia. Electronic address: ekaterina_b89@list.ru., Umpeleva TV; Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health of the Russian Federation, 50 XXII Partsyezda, 620039, Yekaterinburg, Russia. Electronic address: tumpeleva@ya.ru., Bayborodin SI; The Federal Research Center Institute of Cytology and Genetics, 10 Lavrentyeva Prospect, 630090, Novosibirsk, Russia. Electronic address: bai@bionet.nsc.ru., Vakhrusheva DV; Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health of the Russian Federation, 50 XXII Partsyezda, 620039, Yekaterinburg, Russia. Electronic address: vakhrusheva@urniif.ru., Skornyakov SN; Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health of the Russian Federation, 50 XXII Partsyezda, 620039, Yekaterinburg, Russia. Electronic address: sns@urniif.ru. |
| المصدر: | Tuberculosis (Edinburgh, Scotland) [Tuberculosis (Edinb)] 2018 Sep; Vol. 112, pp. 1-10. Date of Electronic Publication: 2018 Jul 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Churchill Livingstone Country of Publication: Scotland NLM ID: 100971555 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-281X (Electronic) Linking ISSN: 14729792 NLM ISO Abbreviation: Tuberculosis (Edinb) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Edinburgh, New York : Churchill Livingstone, c2001- |
| مواضيع طبية MeSH: | Lung/*microbiology , Macrophages, Alveolar/*microbiology , Mycobacterium tuberculosis/*growth & development , Tuberculosis, Pulmonary/*microbiology, Animals ; Bacterial Load ; Cells, Cultured ; Disease Models, Animal ; Genotype ; Guinea Pigs ; Humans ; Mycobacterium tuberculosis/genetics ; Mycobacterium tuberculosis/pathogenicity ; Virulence |
| مستخلص: | Mycobacterium tuberculosis (Mtb) is an infectious agent that causes tuberculosis (TB) in humans. A study of the volume of Mtb population and the detection of Mtb virulence in the lungs of patients with pulmonary TB are of great importance for understanding the infectious process and the outcome of the disease. We analyzed the functional state of Mtb and their number in alveolar macrophages obtained from the resected lungs of patients with TB in ex vivo culture and determined that the number of Mtb, referred mainly to the Beijing genotype family (A0 and B0/W148 clusters), were significantly different in cells between different patients. Only single Mtb were found in alveolar macrophages of some patients, while Mtb were actively replicated in colonies in alveolar macrophages of other patients, including cord morphology of Mtb growth (the indicator of Mtb virulence). Our data demonstrated association between the formation of Mtb cording in alveolar macrophages of patients and increased virulence of Mtb from the lungs of these patients in guinea pig TB model. The find of cording formation by replicating Mtb in human alveolar macrophages may be used for preliminary quick estimation of increased Mtb virulence in individual patients with pulmonary TB. (Copyright © 2018 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Alveolar macrophages; Guinea pigs; Mycobacterial cording; Mycobacterium tuberculosis; Tuberculosis patients; Virulence |
| تواريخ الأحداث: | Date Created: 20180913 Date Completed: 20190903 Latest Revision: 20190903 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.tube.2018.07.001 |
| PMID: | 30205961 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1873-281X |
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| DOI: | 10.1016/j.tube.2018.07.001 |