دورية أكاديمية
Arabidopsis PIRL6 Is Essential for Male and Female Gametogenesis and Is Regulated by Alternative Splicing.
| العنوان: | Arabidopsis PIRL6 Is Essential for Male and Female Gametogenesis and Is Regulated by Alternative Splicing. |
|---|---|
| المؤلفون: | Forsthoefel NR; Program in Biochemistry, Biophysics, and Molecular Biology, Whitman College, Walla Walla, Washington 99362., Klag KA; Program in Biochemistry, Biophysics, and Molecular Biology, Whitman College, Walla Walla, Washington 99362., McNichol SR; Program in Biochemistry, Biophysics, and Molecular Biology, Whitman College, Walla Walla, Washington 99362., Arnold CE; Program in Biochemistry, Biophysics, and Molecular Biology, Whitman College, Walla Walla, Washington 99362., Vernon CR; Program in Biochemistry, Biophysics, and Molecular Biology, Whitman College, Walla Walla, Washington 99362., Wood WW; Program in Biochemistry, Biophysics, and Molecular Biology, Whitman College, Walla Walla, Washington 99362., Vernon DM; Program in Biochemistry, Biophysics, and Molecular Biology, Whitman College, Walla Walla, Washington 99362 vernondm@whitman.edu. |
| المصدر: | Plant physiology [Plant Physiol] 2018 Nov; Vol. 178 (3), pp. 1154-1169. Date of Electronic Publication: 2018 Sep 11. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society of Plant Biologists Country of Publication: United States NLM ID: 0401224 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2548 (Electronic) Linking ISSN: 00320889 NLM ISO Abbreviation: Plant Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Original Publication: Lancaster, Pa., American Society of Plant Physiologists. |
| مواضيع طبية MeSH: | Adaptor Proteins, Signal Transducing/*metabolism , Alternative Splicing/*genetics , Arabidopsis/*genetics , Arabidopsis Proteins/*metabolism , Gametogenesis, Plant/*genetics , Ovule/*genetics , Pollen/*genetics, Adaptor Proteins, Signal Transducing/genetics ; Arabidopsis/physiology ; Arabidopsis/ultrastructure ; Arabidopsis Proteins/genetics ; Codon, Nonsense/genetics ; DNA, Complementary/genetics ; Gene Knockout Techniques ; Genes, Reporter ; Leucine-Rich Repeat Proteins ; Mutation ; Organ Specificity ; Ovule/physiology ; Ovule/ultrastructure ; Plants, Genetically Modified ; Pollen/physiology ; Pollen/ultrastructure ; Proteins ; RNA, Messenger/genetics |
| مستخلص: | Plant intracellular Ras-group leucine-rich repeat (LRR) proteins (PIRLs) are related to Ras-interacting animal LRR proteins that participate in developmental cell signaling. Systematic knockout analysis has implicated some members of the Arabidopsis ( Arabidopsis thaliana ) PIRL family in pollen development. However, for PIRL6 , no bona fide knockout alleles have been recovered, suggesting that it may have an essential function in both male and female gametophytes. To test this hypothesis, we investigated PIRL6 expression and induced knockdown by RNA interference. Knockdown triggered defects in gametogenesis, resulting in abnormal pollen and early developmental arrest in the embryo sac. Consistent with this, PIRL6 was expressed in gametophytes: functional transcripts were detected in wild-type flowers but not in sporocyteless ( spl ) mutant flowers, which do not produce gametophytes. A genomic PIRL6-GFP fusion construct confirmed expression in both pollen and the embryo sac. Interestingly, PIRL6 is part of a convergent overlapping gene pair, a scenario associated with an increased likelihood of alternative splicing. We detected multiple alternative PIRL6 mRNAs in vegetative organs and spl mutant flowers, tissues that lacked the functionally spliced transcript. cDNA sequencing revealed that all contained intron sequences and premature termination codons. These alternative mRNAs accumulated in the nonsense-mediated decay mutant upf3 , indicating that they are normally subjected to degradation. Together, these results demonstrate that PIRL6 is required in both male and female gametogenesis and suggest that sporophytic expression is negatively regulated by unproductive alternative splicing. This posttranscriptional mechanism may function to minimize PIRL6 protein expression in sporophyte tissues while allowing the overlapping adjacent gene to remain widely transcribed. (© 2018 American Society of Plant Biologists. All rights reserved.) |
| References: | Curr Biol. 2010 Mar 23;20(6):506-12. (PMID: 20226671) Arabidopsis Book. 2011;9:e0155. (PMID: 22303279) Plant Physiol. 2001 Aug;126(4):1527-38. (PMID: 11500551) Mol Gen Genet. 1998 Dec;260(5):444-52. (PMID: 9894914) Plant Physiol. 2008 Nov;148(3):1201-11. (PMID: 18775970) Eur J Cell Biol. 2008 Sep;87(8-9):721-34. (PMID: 18436335) Development. 2005 Feb;132(3):603-14. (PMID: 15634699) J Biol Chem. 2009 Oct 23;284(43):29298-309. (PMID: 19720835) Plant J. 2006 Aug;47(3):480-9. (PMID: 16813578) Prog Biophys Mol Biol. 1996;65(1-2):1-44. (PMID: 9029940) Plant J. 2004 Sep;39(5):761-75. (PMID: 15315637) J Cell Sci. 2001 Feb;114(Pt 3):549-62. (PMID: 11171324) Plant Mol Biol. 2016 Jun;91(3):287-304. (PMID: 26969016) Plant Cell. 2004;16 Suppl:S133-41. (PMID: 15075395) J Mol Biol. 1998 Apr 3;277(3):519-27. (PMID: 9533877) Transgenic Res. 2007 Dec;16(6):771-81. (PMID: 17273915) Plant Physiol. 2012 Mar;158(3):1115-29. (PMID: 22247268) Plant Physiol. 2012 Jan;158(1):67-77. (PMID: 22045923) PLoS One. 2012;7(11):e49309. (PMID: 23145149) Genome Res. 2010 Jan;20(1):45-58. (PMID: 19858364) J Biol Chem. 2010 Nov 26;285(48):37281-92. (PMID: 20870712) Plant J. 1998 Dec;16(6):735-43. (PMID: 10069079) Plant Physiol. 2007 Oct;145(2):330-3. (PMID: 17911649) J Exp Bot. 2011 Mar;62(5):1593-9. (PMID: 21118822) Planta. 2010 Oct;232(5):1101-14. (PMID: 20697737) Nucleic Acids Res. 2006 Jan 1;34(Database issue):D731-5. (PMID: 16381968) Mol Cell Biol. 1992 Sep;12(9):3750-6. (PMID: 1508180) PLoS Genet. 2009 Aug;5(8):e1000621. (PMID: 19714218) Plant J. 2010 Mar;61(6):928-40. (PMID: 20409268) Plant J. 2010 Dec;64(6):990-1001. (PMID: 21143679) Plant Cell. 2004;16 Suppl:S142-53. (PMID: 15037731) Genetics. 2004 Oct;168(2):971-82. (PMID: 15514068) FEBS Lett. 2004 May 21;566(1-3):223-8. (PMID: 15147899) PLoS One. 2011;6(12):e28398. (PMID: 22164284) Plant Cell Physiol. 2005 Jun;46(6):913-22. (PMID: 15809230) Plant Physiol. 2007 Jul;144(3):1247-55. (PMID: 17496106) Mol Biol Evol. 1995 May;12(3):405-14. (PMID: 7739382) Plant Biol (Stuttg). 2011 Sep;13(5):784-93. (PMID: 21815983) Genetics. 1997 Nov;147(3):1411-22. (PMID: 9383081) Plant J. 2016 Jul;87(2):188-201. (PMID: 27121542) J Exp Bot. 2009;60(5):1465-78. (PMID: 19213812) Genetics. 1998 Jun;149(2):621-31. (PMID: 9611178) Annu Rev Plant Biol. 2011;62:461-84. (PMID: 21332359) Genetics. 2016 Jun;203(2):817-29. (PMID: 27075727) Dev Biol. 1998 Oct 1;202(1):136-51. (PMID: 9758709) Mol Cell Biol. 2004 Mar;24(5):2103-17. (PMID: 14966289) Genome Biol. 2005;6(6):R51. (PMID: 15960803) PLoS One. 2012;7(1):e29685. (PMID: 22291893) Genetics. 2009 Apr;181(4):1369-85. (PMID: 19237690) Genome Biol. 2004;5(11):R85. (PMID: 15535861) Cell. 1998 Jul 10;94(1):119-30. (PMID: 9674433) Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):189-92. (PMID: 12502788) Plant J. 2017 Apr;90(2):261-275. (PMID: 28107777) Plant J. 2005 Dec;44(5):866-78. (PMID: 16297076) J Exp Bot. 2017 Feb 1;68(4):785-796. (PMID: 27702990) New Phytol. 2010 Jan;185(1):83-9. (PMID: 19863731) Plant Physiol. 2013 Apr;161(4):2171-80. (PMID: 23457227) BMC Plant Biol. 2010 Dec 17;10:280. (PMID: 21167045) Plant J. 2006 Feb;45(3):347-57. (PMID: 16412082) Plants (Basel). 2013 Aug 09;2(3):507-20. (PMID: 27137390) Genes Dev. 2000 Apr 15;14(8):895-900. (PMID: 10783161) Sex Plant Reprod. 2011 Jun;24(2):113-21. (PMID: 21298290) Biochem Soc Trans. 2010 Apr;38(2):593-7. (PMID: 20298227) Dev Biol. 1994 Oct;165(2):566-73. (PMID: 7958422) Plant J. 2011 Feb;65(4):503-16. (PMID: 21208307) Genes Dev. 1999 Aug 15;13(16):2108-17. (PMID: 10465788) Planta. 2005 Jun;221(3):424-36. (PMID: 15647901) J Cell Commun Signal. 2013 Dec;7(4):279-93. (PMID: 23765260) Plant Mol Biol. 1998 Feb;36(3):463-71. (PMID: 9484486) Plant Mol Biol. 2010 Dec;74(6):617-29. (PMID: 20949308) Trends Plant Sci. 2008 Sep;13(9):483-91. (PMID: 18684657) Mol Cell. 2013 Dec 12;52(5):679-92. (PMID: 24211266) Plant J. 2014 Nov;80(4):615-28. (PMID: 25182975) Methods Enzymol. 2005;392:1-24. (PMID: 15644172) Curr Opin Struct Biol. 2001 Dec;11(6):725-32. (PMID: 11751054) Oncogene. 2007 May 14;26(22):3113-21. (PMID: 17496910) Plant Physiol. 2013 Jun;162(2):1092-109. (PMID: 23590974) Planta. 2011 Feb;233(2):423-31. (PMID: 21140166) Plant Physiol. 2007 Apr;143(4):1534-46. (PMID: 17322334) Nature. 2010 Mar 25;464(7288):628-32. (PMID: 20208518) |
| المشرفين على المادة: | 0 (Adaptor Proteins, Signal Transducing) 0 (Arabidopsis Proteins) 0 (Codon, Nonsense) 0 (DNA, Complementary) 0 (Leucine-Rich Repeat Proteins) 0 (Proteins) 0 (RNA, Messenger) 0 (pirl9 protein, Arabidopsis) |
| تواريخ الأحداث: | Date Created: 20180913 Date Completed: 20190328 Latest Revision: 20211204 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC6236607 |
| DOI: | 10.1104/pp.18.00329 |
| PMID: | 30206104 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1532-2548 |
|---|---|
| DOI: | 10.1104/pp.18.00329 |