دورية أكاديمية
أعرض في EDS

Photobiomodulation is associated with a decrease in cell viability and migration in oral squamous cell carcinoma.

التفاصيل البيبلوغرافية
العنوان: Photobiomodulation is associated with a decrease in cell viability and migration in oral squamous cell carcinoma.
المؤلفون: Schalch TD; Biophotonics Applied to Health Sciences Postgraduate Program, Nove de Julho University - UNINOVE, 235/249 Vergueiro Street, São Paulo, 01504-001, Brazil., Fernandes MH; Laboratory for Bone Metabolism and Regeneration; Faculdade de Medicina Dentária, Universidade do Porto, Dr. Manuel Pereira da Silva Street,, 4200-393, Porto, Portugal., Destro Rodrigues MFS; Biophotonics Applied to Health Sciences Postgraduate Program, Nove de Julho University - UNINOVE, 235/249 Vergueiro Street, São Paulo, 01504-001, Brazil., Guimarães DM; Department of Oral Pathology, School of Dentistry, University of São Paulo, 2227 Professor Lineu Prestes Avenue,, São Paulo, 05508-000, Brazil., Nunes FD; Department of Oral Pathology, School of Dentistry, University of São Paulo, 2227 Professor Lineu Prestes Avenue,, São Paulo, 05508-000, Brazil., Rodrigues JC; Laboratory for Bone Metabolism and Regeneration; Faculdade de Medicina Dentária, Universidade do Porto, Dr. Manuel Pereira da Silva Street,, 4200-393, Porto, Portugal., Garcia MP; Biophotonics Applied to Health Sciences Postgraduate Program, Nove de Julho University - UNINOVE, 235/249 Vergueiro Street, São Paulo, 01504-001, Brazil.; Laboratory for Bone Metabolism and Regeneration; Faculdade de Medicina Dentária, Universidade do Porto, Dr. Manuel Pereira da Silva Street,, 4200-393, Porto, Portugal., Mesquita Ferrari RA; Biophotonics Applied to Health Sciences Postgraduate Program, Nove de Julho University - UNINOVE, 235/249 Vergueiro Street, São Paulo, 01504-001, Brazil., Bussadori SK; Biophotonics Applied to Health Sciences Postgraduate Program, Nove de Julho University - UNINOVE, 235/249 Vergueiro Street, São Paulo, 01504-001, Brazil., Fernandes KPS; Biophotonics Applied to Health Sciences Postgraduate Program, Nove de Julho University - UNINOVE, 235/249 Vergueiro Street, São Paulo, 01504-001, Brazil. kristianneporta@gmail.com.
المصدر: Lasers in medical science [Lasers Med Sci] 2019 Apr; Vol. 34 (3), pp. 629-636. Date of Electronic Publication: 2018 Sep 19.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: England NLM ID: 8611515 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1435-604X (Electronic) Linking ISSN: 02688921 NLM ISO Abbreviation: Lasers Med Sci Subsets: MEDLINE
أسماء مطبوعة: Publication: London : Springer
Original Publication: London : Baillière Tindall, c1986-
مواضيع طبية MeSH: Cell Movement*/radiation effects , Low-Level Light Therapy*, Carcinoma, Squamous Cell/*pathology , Carcinoma, Squamous Cell/*radiotherapy , Mouth Neoplasms/*pathology , Mouth Neoplasms/*radiotherapy, Apoptosis/radiation effects ; Caspases/metabolism ; Cell Line, Tumor ; Cell Proliferation/radiation effects ; Cell Survival/radiation effects ; Humans ; Mitochondria/metabolism ; Mitochondria/radiation effects
مستخلص: The treatment of squamous cell carcinoma (SCC) involves surgery, chemotherapy, and/or radiotherapy, which can cause mucositis (inflammation of the oral mucosa that causes considerable pain and can compromise the continuity of oncological treatment). Photobiomodulation (PBM) has been successfully used in the treatment of mucositis, but doubts arise regarding the use of laser for areas in which tumor cells may remain. In this study, the effect of PBM on the viability, mitochondrial activity, proliferation, apoptosis, and migration of cells derived from oral SCC was evaluated. SCC9 cells were irradiated with laser (660 and 780 nm, using 11 dosimetric parameters) and submitted to mitochondrial and caspase 3 activity tests after 1 and 3 days. Based on the results, cell viability (neutral red assay), proliferation (BrdU assay), and migration (scratch-wound assay) were evaluated using only the dosimetric parameters recommended for mucositis. Non-irradiated cells served as the control. The experiments were performed in triplicate. The 11 parameters diminished mitochondrial activity and induced tumor cell apoptosis. Using the parameters recommended for mucositis, irradiation with 780 nm (70 mW, 4 J/cm 2 ) proved to be the safest and led to a reduction in cell viability, the induction of apoptosis, and a reduction in the migration capacity of the tumor cells.
References: J Clin Laser Med Surg. 2002 Feb;20(1):23-6. (PMID: 11902350)
J Pathol. 2003 Oct;201(2):181-6. (PMID: 14517834)
Toxicol Lett. 2006 Jan 5;160(2):171-7. (PMID: 16111842)
Ann N Y Acad Sci. 2005 Nov;1056:486-93. (PMID: 16387711)
Lasers Surg Med. 2006 Apr;38(4):332-6. (PMID: 16526043)
BMC Cancer. 2006 Jul 25;6:199. (PMID: 16869970)
J Immunol Methods. 1991 Dec 15;145(1-2):259-62. (PMID: 1765661)
Med Oral Patol Oral Cir Bucal. 2008 Mar 01;13(3):E189-92. (PMID: 18305441)
Carcinogenesis. 2008 Aug;29(8):1483-92. (PMID: 18310089)
Support Care Cancer. 2008 Dec;16(12):1381-7. (PMID: 18458964)
Oncol Rep. 2008 Sep;20(3):663-8. (PMID: 18695921)
Oncologist. 2008 Aug;13(8):886-98. (PMID: 18701763)
Cancer Lett. 2009 Feb 8;274(1):126-31. (PMID: 18930344)
Semin Radiat Oncol. 2009 Jan;19(1):29-34. (PMID: 19028343)
Int J Oral Maxillofac Surg. 2009 Mar;38(3):279-84. (PMID: 19223152)
J Biomed Sci. 2009 Jan 12;16:4. (PMID: 19272168)
Lasers Surg Med. 2009 Apr;41(4):264-70. (PMID: 19347940)
J Oral Pathol Med. 2009 Nov;38(10):753-8. (PMID: 19566744)
Dose Response. 2009 Sep 01;7(4):358-83. (PMID: 20011653)
J Oral Sci. 2010 Jun;52(2):267-73. (PMID: 20587952)
Toxicol In Vitro. 1998 Jun 1;12(3):251-8. (PMID: 20654407)
Lasers Med Sci. 2012 Jan;27(1):237-49. (PMID: 21274733)
Support Care Cancer. 2012 Mar;20(3):523-9. (PMID: 21340656)
Oral Dis. 2011 Jul;17(5):462-8. (PMID: 21496184)
Toxicol In Vitro. 2011 Dec;25(8):2147-51. (PMID: 21798338)
Oncology. 2011;81(1):12-20. (PMID: 21912193)
Ann Biomed Eng. 2012 Feb;40(2):516-33. (PMID: 22045511)
Lasers Med Sci. 2012 Nov;27(6):1189-93. (PMID: 22190155)
Curr Opin Oncol. 2012 Jul;24(4):363-70. (PMID: 22450151)
Dose Response. 2011;9(4):602-18. (PMID: 22461763)
Photochem Photobiol. 2012 Sep-Oct;88(5):1211-6. (PMID: 22540924)
Photomed Laser Surg. 2012 Sep;30(9):551-8. (PMID: 22853435)
J Biophotonics. 2013 Oct;6(10):839-47. (PMID: 23554211)
Proc Biol Sci. 2013 Apr 03;280(1759):20122987. (PMID: 23554393)
Histochem Cell Biol. 2014 Aug;142(2):195-204. (PMID: 24429833)
Lasers Med Sci. 2014 Jul;29(4):1385-95. (PMID: 24526326)
J Photochem Photobiol B. 2014 Nov;140:344-58. (PMID: 25226343)
J Lasers Med Sci. 2014 Winter;5(1):1-7. (PMID: 25606332)
BMC Res Notes. 2015 Feb 20;8:47. (PMID: 25884200)
Photomed Laser Surg. 2015 Jul;33(7):347-8. (PMID: 26154721)
Oral Oncol. 2016 Mar;54:7-14. (PMID: 26852286)
Support Care Cancer. 2016 Jun;24(6):2781-92. (PMID: 26984240)
Support Care Cancer. 2016 Jun;24(6):2793-805. (PMID: 26984249)
J Biophotonics. 2016 Dec;9(11-12):1136-1147. (PMID: 27089455)
AIMS Biophys. 2017;4(3):337-361. (PMID: 28748217)
Photochem Photobiol. 2018 Mar;94(2):199-212. (PMID: 29164625)
J Immunol Methods. 1983 Dec 16;65(1-2):55-63. (PMID: 6606682)
Growth Regul. 1995 Jun;5(2):69-84. (PMID: 7627094)
Cancer Res. 1996 Sep 1;56(17):4040-3. (PMID: 8752176)
معلومات مُعتمدة: 2013/07502-1 Fapesp; 014703/2013-00 Capes; 305739/2014-0 CNPq; 305905/2014-7 CNPq; 311078/2015-0 CNPq
فهرسة مساهمة: Keywords: Low-level laser therapy; Mouth neoplasms; Mucositis; Oral squamous carcinoma cells; Photobiomodulation
المشرفين على المادة: EC 3.4.22.- (Caspases)
تواريخ الأحداث: Date Created: 20180921 Date Completed: 20190429 Latest Revision: 20200225
رمز التحديث: 20231215
DOI: 10.1007/s10103-018-2640-4
PMID: 30232646
قاعدة البيانات: MEDLINE
الوصف
تدمد:1435-604X
DOI:10.1007/s10103-018-2640-4