دورية أكاديمية
أعرض في EDS

Antimicrobial activities of saponins from Melanthera elliptica and their synergistic effects with antibiotics against pathogenic phenotypes.

التفاصيل البيبلوغرافية
العنوان: Antimicrobial activities of saponins from Melanthera elliptica and their synergistic effects with antibiotics against pathogenic phenotypes.
المؤلفون: Tagousop CN; Research Unit of Environmental and Applied Chemistry, Department of Chemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon., Tamokou JD; Research Unit of Microbiology and Antimicrobial Substances, Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon. jtamokou@yahoo.fr., Kengne IC; Research Unit of Microbiology and Antimicrobial Substances, Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon., Ngnokam D; Research Unit of Environmental and Applied Chemistry, Department of Chemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon. dngnokam@yahoo.fr., Voutquenne-Nazabadioko L; Groupe Isolement et Structure, Institut de Chimie Moléculaire de Reims (ICMR), CNRS, UMR 7312, Bat. 18, BP.1039, 51687, Reims cedex 2, France.
المصدر: Chemistry Central journal [Chem Cent J] 2018 Sep 20; Vol. 12 (1), pp. 97. Date of Electronic Publication: 2018 Sep 20.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101314213 Publication Model: Electronic Cited Medium: Internet ISSN: 1752-153X (Electronic) Linking ISSN: 1752153X NLM ISO Abbreviation: Chem Cent J Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: [London, United Kingdom] : BioMed Central
Original Publication: London : Chemistry Central Ltd., in association with BioMed Central, c2007-
مستخلص: Background: Resistance of bacteria and fungi to antibiotics is one of the biggest problems that faces public health. The present work was designated to evaluate the antimicrobial activities of saponins from Melanthera elliptica and their synergistic effects with standard antibiotics against pathogenic phenotypes. The plant extract was prepared by maceration in methanol. The methanol extract was partitioned into ethyl acetate and n-butanol extracts. Column chromatography of the n-butanol extract followed by purification of different fractions led to the isolation of four saponins. Their structures were elucidated on the basis of spectra analysis, and by comparison with those from the literature. The antimicrobial activities of the extracts/compounds alone and their combinations with tetracycline and fluconazole were evaluated using the broth microdilution method through the determination of minimum inhibitory concentration (MIC) and minimum microbicidal concentration.
Results: Four compounds: 3-O-β-D-glucuronopyranosyl-oleanolic acid (1), 3-O-β-D-glucuronopyranosyloleanolic acid 28-O-β-D-glucopyranosyl ester (2), 3-O-β-D-glucopyranosyl(1 → 2)-β-D-glucuronopyranosyl oleanolic acid (3) and 3-O-β-D-glucopyranosyl(1 → 2)-β-D-glucuronopyranosyl oleanolic acid 28-O-β-D-glucopyranosyl ester (4) were isolated. Compounds 1, 2 and 3 showed the largest antibacterial activities (MIC = 8-128 μg/mL) whereas compound 4 displayed the highest antifungal activities (MIC = 8-16 μg/mL). The antibacterial activities of compounds 1 and 2 (MIC = 16-32 μg/mL) against multi-drug-resistant Escherichia coli S2 (1) and Shigella flexneri SDINT are equal to those of vancomycin (MIC = 16-32 μg/mL) used as reference antibiotic.
Conclusions: The present study showed significant antimicrobial activity of compounds 1, 2, 3 and 4 against the tested microorganisms. The saponins act in synergy with the tested standard antibiotics. This synergy could lead to new options for the treatment of infectious diseases and emerging drug resistance.
References: Biol Pharm Bull. 2008 Aug;31(8):1614-7. (PMID: 18670099)
Springerplus. 2014 Jun 23;3:302. (PMID: 25279277)
Iran J Med Sci. 2011 Mar;36(1):24-31. (PMID: 23365474)
Lett Appl Microbiol. 1998 Jul;27(1):35-8. (PMID: 9722995)
J Antimicrob Chemother. 2011 Jun;66(6):1374-82. (PMID: 21436153)
J Pharm Sci. 1998 Nov;87(11):1395-9. (PMID: 9811496)
J Med Microbiol. 2015 Aug;64(8):901-9. (PMID: 26272388)
Pathogens. 2014 Jun 18;3(2):473-98. (PMID: 25437810)
BMC Complement Altern Med. 2014 Oct 02;14:369. (PMID: 25277679)
Asian Pac J Trop Biomed. 2012 Feb;2(2):144-8. (PMID: 23569885)
BMC Complement Altern Med. 2011 Nov 01;11:104. (PMID: 22044718)
J Agric Food Chem. 2001 May;49(5):2327-32. (PMID: 11368598)
Lancet Infect Dis. 2011 May;11(5):344; author reply 344-5. (PMID: 21530889)
Int J Mol Sci. 2012;13(7):8915-32. (PMID: 22942742)
Planta Med. 1998 Jun;64(5):404-7. (PMID: 9690340)
J Microbiol Methods. 2009 May;77(2):178-83. (PMID: 19318050)
Arch Intern Med. 1994 Jan 10;154(1):26-34. (PMID: 8267486)
BMC Complement Altern Med. 2015 Apr 28;15:134. (PMID: 25928352)
J Med Microbiol. 2011 Sep;60(Pt 9):1337-43. (PMID: 21511888)
BMC Complement Altern Med. 2016 Nov 14;16(1):462. (PMID: 27842536)
فهرسة مساهمة: Keywords: Additive; Antibacterial; Antifungal; Asteraceae; Melanthera elliptica; Saponins; Synergy
تواريخ الأحداث: Date Created: 20180922 Latest Revision: 20201001
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC6768134
DOI: 10.1186/s13065-018-0466-6
PMID: 30238231
قاعدة البيانات: MEDLINE
الوصف
تدمد:1752-153X
DOI:10.1186/s13065-018-0466-6