دورية أكاديمية
Chimeric Antigen Receptor-T Cells with 4-1BB Co-Stimulatory Domain Present a Superior Treatment Outcome than Those with CD28 Domain Based on Bioinformatics.
| العنوان: | Chimeric Antigen Receptor-T Cells with 4-1BB Co-Stimulatory Domain Present a Superior Treatment Outcome than Those with CD28 Domain Based on Bioinformatics. |
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| المؤلفون: | Zhong Q; Department of Hematology, Guangdong Second Provincial General Hospital, Guangzhou, China., Zhu YM; Department of Hematology, Guangdong Second Provincial General Hospital, Guangzhou, China., Zheng LL; Department of Hematology, Guangdong Second Provincial General Hospital, Guangzhou, China., Shen HJ; Department of Hematology, Guangdong Second Provincial General Hospital, Guangzhou, China., Ou RM; Department of Hematology, Guangdong Second Provincial General Hospital, Guangzhou, China., Liu Z; Department of Hematology, Guangdong Second Provincial General Hospital, Guangzhou, China., She YL; Guangdong Traditional Medical and Sports Injury Rehabilitation Research Institute, Guangdong Second Provincial General Hospital, Guangzhou, China., Chen R; Guangdong Traditional Medical and Sports Injury Rehabilitation Research Institute, Guangdong Second Provincial General Hospital, Guangzhou, China., Li C; Guangdong Traditional Medical and Sports Injury Rehabilitation Research Institute, Guangdong Second Provincial General Hospital, Guangzhou, China., Huang J; Department of Hematology, Guangdong Second Provincial General Hospital, Guangzhou, China., Yao MD; Department of Hematology, Guangdong Second Provincial General Hospital, Guangzhou, China., Zhang Q; Department of Hematology, Guangdong Second Provincial General Hospital, Guangzhou, China., Liu S; Department of Hematology, Guangdong Second Provincial General Hospital, Guangzhou, China. |
| المصدر: | Acta haematologica [Acta Haematol] 2018; Vol. 140 (3), pp. 131-140. Date of Electronic Publication: 2018 Sep 25. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Karger Country of Publication: Switzerland NLM ID: 0141053 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1421-9662 (Electronic) Linking ISSN: 00015792 NLM ISO Abbreviation: Acta Haematol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Karger. |
| مواضيع طبية MeSH: | CD28 Antigens/*metabolism , Computational Biology/*methods , Receptors, Chimeric Antigen/*metabolism , Tumor Necrosis Factor Receptor Superfamily, Member 9/*metabolism, Antineoplastic Agents/therapeutic use ; Databases, Factual ; Gene Expression Regulation, Neoplastic ; Humans ; MicroRNAs/metabolism ; Precursor Cell Lymphoblastic Leukemia-Lymphoma/drug therapy ; Protein Interaction Maps/genetics ; Receptors, Chimeric Antigen/chemistry ; Treatment Outcome ; Tumor Necrosis Factor Receptor Superfamily, Member 9/chemistry |
| مستخلص: | Background: The second-generation CD19-chimeric antigen receptor (CAR)-T co-stimulatory domain that is commonly used in clinical practice is CD28 or 4-1BB. Previous studies have shown that the persistence of CAR-T in the 4-1BB co-stimulatory domain appears to be longer. Methods: The expression profile data of GSE65856 were obtained from GEO database. After data preprocessing, the differentially expressed genes (DEGs) between the mock CAR versus CD19-28z CAR T cells and mock CAR versus CD19-BBz CAR T cells were identified using the limma package. Subsequently, functional enrichment analysis of DEGs was performed using the DAVID tool. Then, the protein-protein international (PPI) network of these DEGs was visualized by Cytoscape, and the miRNA-target gene-disease regulatory networks were predicted using Webgestal. Results: A total of 18 common DEGs, 6 CD19-28z specific DEGs and 206 CD19-BBz specific DEGs were identified. Among CD19-28z specific DEGs, down-regulated PAX5 might be an important node in the PPI network and could be targeted by miR-496. In CD19-BBz group, JUN was a hub node in the PPI network and involved in the regulations of miR520D - early growth response gene 3 (EGR3)-JUN and mi-R489-AT-rich interaction domain 5A (ARID5A)-JUN networks. Conclusion: The 4-1BB co-stimulatory domain might play in important role in the treatment of CAR-T via miR-520D-EGR3-JUN and miR489-ARID5A-JUN regulation network, while CD28 had a negative effect on CAR-T treatment. (© 2018 S. Karger AG, Basel.) |
| فهرسة مساهمة: | Keywords: CD19-28z; Differentially expressed genes; GD2.BBz; Protein-protein interaction |
| المشرفين على المادة: | 0 (Antineoplastic Agents) 0 (CD28 Antigens) 0 (MicroRNAs) 0 (Receptors, Chimeric Antigen) 0 (Tumor Necrosis Factor Receptor Superfamily, Member 9) |
| تواريخ الأحداث: | Date Created: 20180926 Date Completed: 20190610 Latest Revision: 20190613 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1159/000492146 |
| PMID: | 30253384 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1421-9662 |
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| DOI: | 10.1159/000492146 |