دورية أكاديمية
أعرض في EDS

Control of seminal fluid protein expression via regulatory hubs in Drosophila melanogaster .

التفاصيل البيبلوغرافية
العنوان: Control of seminal fluid protein expression via regulatory hubs in Drosophila melanogaster .
المؤلفون: Mohorianu I; School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK.; School of Computing Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK., Fowler EK; School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK., Dalmay T; School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK., Chapman T; School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK tracey.chapman@uea.ac.uk.
المصدر: Proceedings. Biological sciences [Proc Biol Sci] 2018 Sep 26; Vol. 285 (1887). Date of Electronic Publication: 2018 Sep 26.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Royal Society of London Country of Publication: England NLM ID: 101245157 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2954 (Electronic) Linking ISSN: 09628452 NLM ISO Abbreviation: Proc Biol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Royal Society of London, c1990-
مواضيع طبية MeSH: Drosophila melanogaster/*genetics , Insect Proteins/*metabolism , Semen/*metabolism, Animals ; Drosophila melanogaster/metabolism ; Gene Expression Regulation ; Gene Knockdown Techniques/methods ; Gene Regulatory Networks ; Genetic Fitness ; Insect Proteins/genetics ; Male ; MicroRNAs ; Sexual Behavior, Animal ; Transcription Factors
مستخلص: Highly precise, yet flexible and responsive coordination of expression across groups of genes underpins the integrity of many vital functions. However, our understanding of gene regulatory networks (GRNs) is often hampered by the lack of experimentally tractable systems, by significant computational challenges derived from the large number of genes involved or from difficulties in the accurate identification and characterization of gene interactions. Here we used a tractable experimental system in which to study GRNs: the genes encoding the seminal fluid proteins that are transferred along with sperm (the 'transferome') in Drosophila melanogaster fruit flies. The products of transferome genes are core determinants of reproductive success and, to date, only transcription factors have been implicated in the modulation of their expression. Hence, as yet, we know nothing about the post-transcriptional mechanisms underlying the tight, responsive and precise regulation of this important gene set. We investigated this omission in the current study. We first used bioinformatics to identify potential regulatory motifs that linked the transferome genes in a putative interaction network. This predicted the presence of putative microRNA (miRNA) 'hubs'. We then tested this prediction, that post-transcriptional regulation is important for the control of transferome genes, by knocking down miRNA expression in adult males. This abolished the ability of males to respond adaptively to the threat of sexual competition, indicating a regulatory role for miRNAs in the regulation of transferome function. Further bioinformatics analysis then identified candidate miRNAs as putative regulatory hubs and evidence for variation in the strength of miRNA regulation across the transferome gene set. The results revealed regulatory mechanisms that can underpin robust, precise and flexible regulation of multiple fitness-related genes. They also help to explain how males can adaptively modulate ejaculate composition.
(© 2018 The Authors.)
References: Nat Rev Mol Cell Biol. 2009 Feb;10(2):126-39. (PMID: 19165215)
Interface Focus. 2011 Dec 6;1(6):857-70. (PMID: 23226586)
BMC Evol Biol. 2014 Apr 04;14:74. (PMID: 24707827)
Cold Spring Harb Perspect Biol. 2014 Dec 11;7(2):a017533. (PMID: 25502515)
Insect Biochem Mol Biol. 2014 Jun;49:49-58. (PMID: 24721205)
Methods Mol Biol. 2017;1580:175-191. (PMID: 28439834)
Integr Comp Biol. 2007 Sep;47(3):427-45. (PMID: 21672851)
Proc Biol Sci. 2012 Jul 22;279(1739):2868-76. (PMID: 22438501)
Proc Biol Sci. 2009 May 7;276(1662):1705-11. (PMID: 19324834)
Curr Biol. 2009 May 12;19(9):751-7. (PMID: 19361995)
Mol Cell. 2011 Sep 16;43(6):892-903. (PMID: 21925378)
Am Nat. 2018 Aug;192(2):217-229. (PMID: 30016167)
Heredity (Edinb). 2001 Nov;87(Pt 5):511-21. (PMID: 11869341)
Proc Natl Acad Sci U S A. 2003 Aug 19;100(17):9929-33. (PMID: 12897240)
PLoS Comput Biol. 2008 Jul 11;4(7):e1000112. (PMID: 18617989)
Genes Brain Behav. 2014 Jan;13(1):13-24. (PMID: 24320616)
Cell. 2006 Dec 15;127(6):1209-21. (PMID: 17174895)
Nucleic Acids Res. 2014 Jan;42(Database issue):D68-73. (PMID: 24275495)
Nature. 2004 Sep 16;431(7006):308-12. (PMID: 15372033)
BMC Biol. 2012 Jun 21;10:56. (PMID: 22715942)
Genome Res. 2011 May;21(5):645-57. (PMID: 21324878)
IEEE/ACM Trans Comput Biol Bioinform. 2011 Jan-Mar;8(1):94-107. (PMID: 21071800)
J Evol Biol. 2009 Feb;22(2):275-86. (PMID: 19032499)
Bioinformatics. 2015 Jun 15;31(12):i197-205. (PMID: 26072483)
Nat Rev Genet. 2004 Feb;5(2):101-13. (PMID: 14735121)
Genome Res. 2018 Jan;28(1):52-65. (PMID: 29233922)
Genes Dev. 1988 Sep;2(9):1063-73. (PMID: 3142802)
Development. 2015 Oct 1;142(19):3394-402. (PMID: 26443637)
RNA. 2017 Jul;23(7):1048-1059. (PMID: 28428330)
Curr Biol. 2005 Feb 22;15(4):316-21. (PMID: 15723791)
Biol Lett. 2009 Oct 23;5(5):671-4. (PMID: 19574282)
Development. 2008 Jan;135(1):53-63. (PMID: 18045836)
Evolution. 2011 Feb;65(2):584-90. (PMID: 21271997)
Curr Biol. 2011 Apr 12;21(7):617-22. (PMID: 21439827)
Curr Biol. 2013 Nov 18;23(22):2319-2324. (PMID: 24210616)
PLoS Biol. 2008 Jul 29;6(7):e178. (PMID: 18666829)
Curr Top Dev Biol. 2012;99:201-235. (PMID: 22365740)
Mech Dev. 1992 Jul;38(1):33-40. (PMID: 1525037)
Science. 2002 Aug 30;297(5586):1551-5. (PMID: 12202830)
Genome Res. 2003 Nov;13(11):2498-504. (PMID: 14597658)
Proc Biol Sci. 2012 Nov 7;279(1746):4423-32. (PMID: 22977156)
J Exp Bot. 2015 Aug;66(15):4551-66. (PMID: 26022256)
PLoS Biol. 2008 Jul 29;6(7):e179. (PMID: 18666830)
Cell. 2009 Jan 23;136(2):215-33. (PMID: 19167326)
Proc Natl Acad Sci U S A. 2003 Aug 19;100(17):9923-8. (PMID: 12893873)
Proc Natl Acad Sci U S A. 2011 Jun 14;108(24):9922-6. (PMID: 21628597)
PLoS Biol. 2005 Mar;3(3):e85. (PMID: 15723116)
Genome Res. 2011 Feb;21(2):203-15. (PMID: 21177969)
Insect Biochem Mol Biol. 2012 Aug;42(8):545-56. (PMID: 22580186)
EXCLI J. 2015 Mar 02;14:346-78. (PMID: 27047314)
Dev Dyn. 2009 Apr;238(4):853-63. (PMID: 19253394)
Genes Dev. 2005 Jul 1;19(13):1499-511. (PMID: 15998805)
Science. 2003 Dec 5;302(5651):1727-36. (PMID: 14605208)
Nucleic Acids Res. 2016 Jul 8;44(W1):W83-9. (PMID: 27098042)
PLoS Genet. 2013 Mar;9(3):e1003395. (PMID: 23555301)
J Insect Physiol. 1997 Nov;43(12):1117-1123. (PMID: 12770484)
Proc Biol Sci. 2018 Sep 26;285(1887):. (PMID: 30257913)
Proc Natl Acad Sci U S A. 2007 Aug 21;104(34):13591-6. (PMID: 17690244)
Cell. 2004 Jan 23;116(2):281-97. (PMID: 14744438)
Genome Biol. 2003;5(1):R1. (PMID: 14709173)
Science. 2002 Oct 25;298(5594):824-7. (PMID: 12399590)
معلومات مُعتمدة: BB/H002499/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BB/H008047/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BB/L003139/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council
فهرسة مساهمة: Keywords: gene regulatory network; microRNA (miRNA); regulatory hub; sexual conflict
سلسلة جزيئية: Dryad 10.5061/dryad.fm15b38
figshare 10.6084/m9.figshare.c.4226339
المشرفين على المادة: 0 (Insect Proteins)
0 (MicroRNAs)
0 (Transcription Factors)
تواريخ الأحداث: Date Created: 20180928 Date Completed: 20190912 Latest Revision: 20240402
رمز التحديث: 20240402
مُعرف محوري في PubMed: PMC6170815
DOI: 10.1098/rspb.2018.1681
PMID: 30257913
قاعدة البيانات: MEDLINE
الوصف
تدمد:1471-2954
DOI:10.1098/rspb.2018.1681