دورية أكاديمية
Control of seminal fluid protein expression via regulatory hubs in Drosophila melanogaster .
العنوان: | Control of seminal fluid protein expression via regulatory hubs in Drosophila melanogaster . |
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المؤلفون: | Mohorianu I; School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK.; School of Computing Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK., Fowler EK; School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK., Dalmay T; School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK., Chapman T; School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK tracey.chapman@uea.ac.uk. |
المصدر: | Proceedings. Biological sciences [Proc Biol Sci] 2018 Sep 26; Vol. 285 (1887). Date of Electronic Publication: 2018 Sep 26. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Royal Society of London Country of Publication: England NLM ID: 101245157 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2954 (Electronic) Linking ISSN: 09628452 NLM ISO Abbreviation: Proc Biol Sci Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: London : Royal Society of London, c1990- |
مواضيع طبية MeSH: | Drosophila melanogaster/*genetics , Insect Proteins/*metabolism , Semen/*metabolism, Animals ; Drosophila melanogaster/metabolism ; Gene Expression Regulation ; Gene Knockdown Techniques/methods ; Gene Regulatory Networks ; Genetic Fitness ; Insect Proteins/genetics ; Male ; MicroRNAs ; Sexual Behavior, Animal ; Transcription Factors |
مستخلص: | Highly precise, yet flexible and responsive coordination of expression across groups of genes underpins the integrity of many vital functions. However, our understanding of gene regulatory networks (GRNs) is often hampered by the lack of experimentally tractable systems, by significant computational challenges derived from the large number of genes involved or from difficulties in the accurate identification and characterization of gene interactions. Here we used a tractable experimental system in which to study GRNs: the genes encoding the seminal fluid proteins that are transferred along with sperm (the 'transferome') in Drosophila melanogaster fruit flies. The products of transferome genes are core determinants of reproductive success and, to date, only transcription factors have been implicated in the modulation of their expression. Hence, as yet, we know nothing about the post-transcriptional mechanisms underlying the tight, responsive and precise regulation of this important gene set. We investigated this omission in the current study. We first used bioinformatics to identify potential regulatory motifs that linked the transferome genes in a putative interaction network. This predicted the presence of putative microRNA (miRNA) 'hubs'. We then tested this prediction, that post-transcriptional regulation is important for the control of transferome genes, by knocking down miRNA expression in adult males. This abolished the ability of males to respond adaptively to the threat of sexual competition, indicating a regulatory role for miRNAs in the regulation of transferome function. Further bioinformatics analysis then identified candidate miRNAs as putative regulatory hubs and evidence for variation in the strength of miRNA regulation across the transferome gene set. The results revealed regulatory mechanisms that can underpin robust, precise and flexible regulation of multiple fitness-related genes. They also help to explain how males can adaptively modulate ejaculate composition. (© 2018 The Authors.) |
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معلومات مُعتمدة: | BB/H002499/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BB/H008047/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BB/L003139/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council |
فهرسة مساهمة: | Keywords: gene regulatory network; microRNA (miRNA); regulatory hub; sexual conflict |
سلسلة جزيئية: | Dryad 10.5061/dryad.fm15b38 figshare 10.6084/m9.figshare.c.4226339 |
المشرفين على المادة: | 0 (Insect Proteins) 0 (MicroRNAs) 0 (Transcription Factors) |
تواريخ الأحداث: | Date Created: 20180928 Date Completed: 20190912 Latest Revision: 20240402 |
رمز التحديث: | 20240402 |
مُعرف محوري في PubMed: | PMC6170815 |
DOI: | 10.1098/rspb.2018.1681 |
PMID: | 30257913 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1471-2954 |
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DOI: | 10.1098/rspb.2018.1681 |