دورية أكاديمية
Cardioprotection of benzolamide in a regional ischemia model: Role of eNOS/NO.
| العنوان: | Cardioprotection of benzolamide in a regional ischemia model: Role of eNOS/NO. |
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| المؤلفون: | González Arbeláez LF; 'Dr Horacio E. Cingolani', Universidad Nacional de La Plata, La Plata, Argentina., Ciocci Pardo A; 'Dr Horacio E. Cingolani', Universidad Nacional de La Plata, La Plata, Argentina., Swenson ER; Department of Medicine, Pulmonary and Critical Care Medicine, VA Puget Sound Health Care System, University of Washington, Seatle, WA 98108, USA., Álvarez BV; 'Dr Horacio E. Cingolani', Universidad Nacional de La Plata, La Plata, Argentina., Mosca SM; 'Dr Horacio E. Cingolani', Universidad Nacional de La Plata, La Plata, Argentina., Fantinelli JC; 'Dr Horacio E. Cingolani', Universidad Nacional de La Plata, La Plata, Argentina. Electronic address: julianafantinelli@ciclaplata.org.ar. |
| المصدر: | Experimental and molecular pathology [Exp Mol Pathol] 2018 Dec; Vol. 105 (3), pp. 345-351. Date of Electronic Publication: 2018 Oct 09. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0370711 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0945 (Electronic) Linking ISSN: 00144800 NLM ISO Abbreviation: Exp Mol Pathol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2000- > : Amsteram : Elsevier Original Publication: New York : Academic Press |
| مواضيع طبية MeSH: | Benzolamide/*pharmacology , Carbonic Anhydrase Inhibitors/*pharmacology , Myocardial Reperfusion Injury/*metabolism , Nitric Oxide/*metabolism , Nitric Oxide Synthase Type III/*metabolism, Animals ; Isolated Heart Preparation ; Male ; Rats ; Rats, Wistar |
| مستخلص: | Background: Recent studies from our laboratory show the cardioprotective action of benzolamide (BZ, carbonic anhydrase inhibitor) against ischemia-reperfusion injury. However, the mechanisms involved have not been fully elucidated. Objective: To examine the participation of the endothelial nitric oxide synthase (eNOS)/nitric oxide (NO) in the effects of BZ in a model of regional ischemia. Methods: Isolated rat hearts perfused by Langendorff technique were submitted to 40 min of coronary artery occlusion followed by 60 min of reperfusion (IC). Other hearts received BZ during the first 10 min of reperfusion in absence or presence of L-NAME, NOS inhibitor. The infarct size (IS) and the post-ischemic recovery of myocardial function were measured. Oxidative/nitrosative damage were assessed by reduced glutathione (GSH) content, thiobarbituric acid reactive substances (TBARS) and 3-nitrotyrosine levels. The expression of phosphorylated forms of Akt, p38MAPK and eNOS, and the concentration of inducible nitric oxide synthase (iNOS) were also determined. Results: BZ significantly decreased IS (6.2 ± 0.5% vs. 34 ± 4%), improved post-ischemic contractility, preserved GSH levels and diminished TBARS and 3-nitrotyrosine. In IC hearts, P-Akt, P-p38MAPK and P-eNOS decreased and iNOS increased. After BZ addition the levels of P-kinases and P-eNOS increased and iNOS decreased. Except for P-Akt, P-p38MAPK and iNOS, the effects of BZ were abolished by L-NAME. Conclusions: Our data demonstrate that the treatment with BZ at the onset of reperfusion was effective to reduce cell death, contractile dysfunction and oxidative/nitrosative damage produced by coronary artery occlusion. These BZ-mediated beneficial actions appear mediated by eNOS/NO-dependent pathways. (Copyright © 2018 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Benzolamide; Carbonic anhydrase inhibition; Coronary artery occlusion; eNOS/NO |
| المشرفين على المادة: | 0 (Carbonic Anhydrase Inhibitors) 31C4KY9ESH (Nitric Oxide) EC 1.14.13.39 (Nitric Oxide Synthase Type III) EC 1.14.13.39 (Nos3 protein, rat) FC5AAH89R5 (Benzolamide) |
| تواريخ الأحداث: | Date Created: 20181012 Date Completed: 20190102 Latest Revision: 20190102 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.yexmp.2018.10.003 |
| PMID: | 30308197 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-0945 |
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| DOI: | 10.1016/j.yexmp.2018.10.003 |